LOCUS       sequence-21905-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  TITLE     Conaway Lab - additional plasmids
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3.1 N-FLAG/Hyg(+)
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(213..230)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    219..443
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(576..595)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tagcagagct ctctggctac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttggt accgagctcg
      121 gatccactag tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgctcga
      181 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
      241 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
      301 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
      361 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
      421 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
      481 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      541 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      601 tctcgccacg ttcgccggct tttnccccca nanctctaaa tcgggggctc cctttagggt
      661 tccgatttat gngctttacg gcacctcgac cccaaaaaac ttgattangg tgatggttca
      721 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga ntccacgttc
      781 ttaatantgg actcttgttc caactga
//
