LOCUS       sequence-219112-        3630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR E709_T710delinsD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-E709_T710delinsD
FEATURES             Location/Qualifiers
     source          1..3630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..72,73..1935,1936..2004,2005..3630)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKDEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPK
                     ANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQY
                     LLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAE
                     GGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGE
                     RLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLP
                     SPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVAC
                     IDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNP
                     VYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISL
                     DNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
ORIGIN
        1 atgcgaccct ccgggacggc cggggcagcg ctcctggcgc tgctggctgc gctctgcccg
       61 gcgagtcggg ctctggagga aaagaaagtt tgccaaggca cgagtaacaa gctcacgcag
      121 ttgggcactt ttgaagatca ttttctcagc ctccagagga tgttcaataa ctgtgaggtg
      181 gtccttggga atttggaaat tacctatgtg cagaggaatt atgatctttc cttcttaaag
      241 accatccagg aggtggctgg ttatgtcctc attgccctca acacagtgga gcgaattcct
      301 ttggaaaacc tgcagatcat cagaggaaat atgtactacg aaaattccta tgccttagca
      361 gtcttatcta actatgatgc aaataaaacc ggactgaagg agctgcccat gagaaattta
      421 caggaaatcc tgcatggcgc cgtgcggttc agcaacaacc ctgccctgtg caacgtggag
      481 agcatccagt ggcgggacat agtcagcagt gactttctca gcaacatgtc gatggacttc
      541 cagaaccacc tgggcagctg ccaaaagtgt gatccaagct gtcccaatgg gagctgctgg
      601 ggtgcaggag aggagaactg ccagaaactg accaaaatca tctgtgccca gcagtgctcc
      661 gggcgctgcc gtggcaagtc ccccagtgac tgctgccaca accagtgtgc tgcaggctgc
      721 acaggccccc gggagagcga ctgcctggtc tgccgcaaat tccgagacga agccacgtgc
      781 aaggacacct gccccccact catgctctac aaccccacca cgtaccagat ggatgtgaac
      841 cccgagggca aatacagctt tggtgccacc tgcgtgaaga agtgtccccg taattatgtg
      901 gtgacagatc acggctcgtg cgtccgagcc tgtggggccg acagctatga gatggaggaa
      961 gacggcgtcc gcaagtgtaa gaagtgcgaa gggccttgcc gcaaagtgtg taacggaata
     1021 ggtattggtg aatttaaaga ctcactctcc ataaatgcta cgaatattaa acacttcaaa
     1081 aactgcacct ccatcagtgg cgatctccac atcctgccgg tggcatttag gggtgactcc
     1141 ttcacacata ctcctcctct ggatccacag gaactggata ttctgaaaac cgtaaaggaa
     1201 atcacagggt ttttgctgat tcaggcttgg cctgaaaaca ggacggacct ccatgccttt
     1261 gagaacctag aaatcatacg cggcaggacc aagcaacatg gtcagttttc tcttgcagtc
     1321 gtcagcctga acataacatc cttgggatta cgctccctca aggagataag tgatggagat
     1381 gtgataattt caggaaacaa aaatttgtgc tatgcaaata caataaactg gaaaaaactg
     1441 tttgggacct ccggtcagaa aaccaaaatt ataagcaaca gaggtgaaaa cagctgcaag
     1501 gccacaggcc aggtctgcca tgccttgtgc tcccccgagg gctgctgggg cccggagccc
     1561 agggactgcg tctcttgccg gaatgtcagc cgaggcaggg aatgcgtgga caagtgcaac
     1621 cttctggagg gtgagccaag ggagtttgtg gagaactctg agtgcataca gtgccaccca
     1681 gagtgcctgc ctcaggccat gaacatcacc tgcacaggac ggggaccaga caactgtatc
     1741 cagtgtgccc actacattga cggcccccac tgcgtcaaga cctgcccggc aggagtcatg
     1801 ggagaaaaca acaccctggt ctggaagtac gcagacgccg gccatgtgtg ccacctgtgc
     1861 catccaaact gcacctacgg atgcactggg ccaggtcttg aaggctgtcc aacgaatggg
     1921 cctaagatcc cgtccatcgc cactgggatg gtgggggccc tcctcttgct gctggtggtg
     1981 gccctgggga tcggcctctt catgcgaagg cgccacatcg ttcggaagcg cacgctgcgg
     2041 aggctgctgc aggagaggga gcttgtggag cctcttacac ccagtggaga agctcccaac
     2101 caagctctct tgaggatctt gaaggatgaa ttcaaaaaga tcaaagtgct gggctccggt
     2161 gcgttcggca cggtgtataa gggactctgg atcccagaag gtgagaaagt taaaattccc
     2221 gtcgctatca aggaattaag agaagcaaca tctccgaaag ccaacaagga aatcctcgat
     2281 gaagcctacg tgatggccag cgtggacaac ccccacgtgt gccgcctgct gggcatctgc
     2341 ctcacctcca ccgtgcagct catcacgcag ctcatgccct tcggctgcct cctggactat
     2401 gtccgggaac acaaagacaa tattggctcc cagtacctgc tcaactggtg tgtgcagatc
     2461 gcaaagggca tgaactactt ggaggaccgt cgcttggtgc accgcgacct ggcagccagg
     2521 aacgtactgg tgaaaacacc gcagcatgtc aagatcacag attttgggct ggccaaactg
     2581 ctgggtgcgg aagagaaaga ataccatgca gaaggaggca aagtgcctat caagtggatg
     2641 gcattggaat caattttaca cagaatctat acccaccaga gtgatgtctg gagctacggg
     2701 gtgaccgttt gggagttgat gacctttgga tccaagccat atgacggaat ccctgccagc
     2761 gagatctcct ccatcctgga gaaaggagaa cgcctccctc agccacccat atgtaccatc
     2821 gatgtctaca tgatcatggt caagtgctgg atgatagacg cagatagtcg cccaaagttc
     2881 cgtgagttga tcatcgaatt ctccaaaatg gcccgagacc cccagcgcta ccttgtcatt
     2941 cagggggatg aaagaatgca tttgccaagt cctacagact ccaacttcta ccgtgccctg
     3001 atggatgaag aagacatgga cgacgtggtg gatgccgacg agtacctcat cccacagcag
     3061 ggcttcttca gcagcccctc cacgtcacgg actcccctcc tgagctctct gagtgcaacc
     3121 agcaacaatt ccaccgtggc ttgcattgat agaaatgggc tgcaaagctg tcccatcaag
     3181 gaagacagct tcttgcagcg atacagctca gaccccacag gcgccttgac tgaggacagc
     3241 atagacgaca ccttcctccc agtgcctgaa tacataaacc agtccgttcc caaaaggccc
     3301 gctggctctg tgcagaatcc tgtctatcac aatcagcctc tgaaccccgc gcccagcaga
     3361 gacccacact accaggaccc ccacagcact gcagtgggca accccgagta tctcaacact
     3421 gtccagccca cctgtgtcaa cagcacattc gacagccctg cccactgggc ccagaaaggc
     3481 agccaccaaa ttagcctgga caaccctgac taccagcagg acttctttcc caaggaagcc
     3541 aagccaaatg gcatctttaa gggctccaca gctgaaaatg cagaatacct aagggtcgcg
     3601 ccacaaagca gtgaatttat tggagcatga
//
