LOCUS       sequence-21921-e         517 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Luong TT, Lee CY
  TITLE     Improved single-copy integration vectors for Staphylococcus aureus.
  JOURNAL   J Microbiol Methods. 2007 Jul . 70(1):186-90.
  PUBMED    17512993
REFERENCE   2  (bases 1 to 517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microbiol
            Methods. 2007 Jul . 70(1):186-90."
COMMENT     Sequence Label: pLL39
FEATURES             Location/Qualifiers
     source          1..517
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    74..130
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      206..292
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      389..475
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 catatgcatt atggcgttgt tccaataact gatgatggtc gtttaagtgc taaagaagtt
       61 gtaggtaata aaaaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga
      121 gctcgaattc gattagatct aagcttcggg aattcactag tgattgggcc cattacagcg
      181 gatcaattcc caattcccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      241 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
      301 agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata
      361 aactgccagg aattaattcc ccaggcatca aataaaacga aaggctcagt cgaaagactg
      421 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
      481 gggagcggat ttgaacgttg cgaagcaacg gcccgga
//
