LOCUS       sequence-219246-        1788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of ESR1 Y537C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ESR1-Y537C
FEATURES             Location/Qualifiers
     source          1..1788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             847..1782
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLCDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             895..1788
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLCDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
ORIGIN
        1 atgaccatga ccctccacac caaagcatct gggatggccc tactgcatca gatccaaggg
       61 aacgagctgg agcccctgaa ccgtccgcag ctcaagatcc ccctggagcg gcccctgggc
      121 gaggtgtacc tggacagcag caagcccgcc gtgtacaact accccgaggg cgccgcctac
      181 gagttcaacg ccgcggccgc cgccaacgcg caggtctacg gtcagaccgg cctcccctac
      241 ggccccgggt ctgaggctgc ggcgttcggc tccaacggcc tggggggttt ccccccactc
      301 aacagcgtgt ctccgagccc gctgatgcta ctgcacccgc cgccgcagct gtcgcctttc
      361 ctgcagcccc acggccagca ggtgccctac tacctggaga acgagcccag cggctacacg
      421 gtgcgcgagg ccggcccgcc ggcattctac aggccaaatt cagataatcg acgccagggt
      481 ggcagagaaa gattggccag taccaatgac aagggaagta tggctatgga atctgccaag
      541 gagactcgct actgtgcagt gtgcaatgac tatgcttcag gctaccatta tggagtctgg
      601 tcctgtgagg gctgcaaggc cttcttcaag agaagtattc aaggacataa cgactatatg
      661 tgtccagcca ccaaccagtg caccattgat aaaaacagga ggaagagctg ccaggcctgc
      721 cggctccgca aatgctacga agtgggaatg atgaaaggtg ggatacgaaa agaccgaaga
      781 ggagggagaa tgttgaaaca caagcgccag agagatgatg gggagggcag gggtgaagtg
      841 gggtctgctg gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc
      901 tctaagaaga acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg
      961 gatgctgagc cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct
     1021 tcgatgatgg gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg
     1081 gcgaagaggg tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa
     1141 tgtgcctggc tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccaggg
     1201 aagctactgt ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc
     1261 atggtggaga tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg
     1321 cagggagagg agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca
     1381 tttctgtcca gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac
     1441 aagatcacag acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag
     1501 caccagcggc tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa
     1561 ggcatggagc atctgtacag catgaagtgc aagaacgtgg tgcccctctg tgacctgctg
     1621 ctggagatgc tggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg
     1681 gaggagacgg accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa
     1741 aagtattaca tcacggggga ggcagagggt ttccctgcca cggtctga
//
