LOCUS       sequence-219305-        4830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of MAP3K4 P27_P28insP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-MAP3K4-P27_P28insP
FEATURES             Location/Qualifiers
     source          1..4830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      58..67
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             1968..1976
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgagagaag ccgctgccgc gctggtccct cctcccgcct ttgccgtcac gcctgccgcc
       61 gccatggagg agccgccgcc accgccgccg ccgccgccac caccgccacc ggaacccgag
      121 accgagtcag aacccgagtg ctgcttggcg gcgaggcaag agggcacatt gggagattca
      181 gcttgcaaga gtcctgaatc tgatctagaa gacttctccg atgaaacaaa tacagagaat
      241 ctttatggta cctctccccc cagcacacct cgacagatga aacgcatgtc aaccaaacat
      301 cagaggaata atgtggggag gccagccagt cggtctaatt tgaaagaaaa aatgaatgca
      361 ccaaatcagc ctccacataa agacactgga aaaacagtgg agaatgtgga agaatacagc
      421 tataagcagg agaaaaagat ccgagcagct cttagaacaa cagagcgtga tcataaaaaa
      481 aatgtacagt gctcattcat gttagactca gtgggtggat ctttgccaaa aaaatcaatt
      541 ccagatgtgg atctcaataa gccttacctc agccttggct gtagcaatgc taagcttcca
      601 gtatctgtgc ccatgcctat agccagacct gcacgccaga cttctaggac tgactgtcca
      661 gcagatcgtt taaagttttt tgaaacttta cgacttttgc taaagcttac ctcagtctca
      721 aagaaaaaag acagggagca aagaggacaa gaaaatacgt ctggtttctg gcttaaccga
      781 tctaacgaac tgatctggtt agagctacaa gcctggcatg caggacggac aattaacgac
      841 caggacttct ttttatatac agcccgtcaa gccatcccag atattattaa tgaaatcctt
      901 actttcaaag tcgactatgg gagcttcgcc tttgttagag atagagctgg ttttaatggt
      961 acttcagtag aagggcagtg caaagccact cctggaacaa agattgtagg ttactcaaca
     1021 catcatgagc atctccaacg ccagagggtc tcatttgagc aggtaaaacg gataatggag
     1081 ctgctagagt acatagaagc actttatcca tcattgcagg ctcttcagaa ggactatgaa
     1141 aaatatgctg caaaagactt ccaggacagg gtgcaggcac tctgtttgtg gttaaacatc
     1201 acaaaagact taaatcagaa attaaggatt atgggcactg ttttgggcat caagaattta
     1261 tcagacattg gctggccagt gtttgaaatc ccttcccctc gaccatccaa aggtaatgag
     1321 ccggagtatg agggtgatga cacagaagga gaattaaagg agttggaaag tagtacggat
     1381 gagagtgaag aagaacaaat ctctgatcct agggtaccgg aaatcagaca gcccatagat
     1441 aacagcttcg acatccagtc gcgggactgc atatccaaga agcttgagag gctcgaatct
     1501 gaggatgatt ctcttggctg gggagcacca gactggagca cagaagcagg ctttagtaga
     1561 cattgtctga cttctattta tagaccattt gtagacaaag cactgaagca gatggggtta
     1621 agaaagttaa ttttaagact tcacaagcta atggatggtt ccttgcaaag ggcacgtata
     1681 gcattggtaa agaacgatcg tccagtggag ttttctgaat ttccagatcc catgtggggt
     1741 tcagattatg tgcagttgtc aaggacacca ccttcatctg aggagaaatg cagtgctgtg
     1801 tcgtgggagg agctgaaggc catggattta ccttcattcg aacctgcctt cctagttctc
     1861 tgccgagtcc ttctgaatgt catacatgag tgtctgaagt taagattgga gcagagacct
     1921 gctggagaac catctctctt gagtattaag cagctggtga gagagtgtaa ggaggtcctg
     1981 aagggcggcc tgctgatgaa gcagtactac cagttcatgc tgcaggaggt tctggaggac
     2041 ttggagaagc ccgactgcaa cattgacgct tttgaagagg atctacataa aatgcttatg
     2101 gtgtattttg attacatgag aagctggatc caaatgctac agcaattacc tcaagcatcg
     2161 catagtttaa aaaatctgtt agaagaagaa tggaatttca ccaaagaaat aactcattac
     2221 atacggggag gagaagcaca ggccgggaag cttttctgtg acattgcagg aatgctgctg
     2281 aaatctacag gaagtttttt agaatttggc ttacaggaga gctgtgctga attttggact
     2341 agtgcggatg acagcagtgc ttccgacgaa atcaggaggt ctgttataga gatcagtcga
     2401 gccctgaagg agctcttcca tgaagccaga gaaagggctt ccaaagcact tggatttgct
     2461 aaaatgttga gaaaggacct ggaaatagca gcagaattca ggctttcagc cccagttaga
     2521 gacctcctgg atgttctgaa atcaaaacag tatgtcaagg tgcaaattcc tgggttagaa
     2581 aacttgcaaa tgtttgttcc agacactctt gctgaggaga agagtattat tttgcagtta
     2641 ctcaatgcag ctgcaggaaa ggactgttca aaagattcag atgacgtact catcgatgcc
     2701 tatctgcttc tgaccaagca cggtgatcga gcccgtgatt cagaggacag ctggggcacc
     2761 tgggaggcac agcctgtcaa agtcgtgcct caggtggaga ctgttgacac cctgagaagc
     2821 atgcaggtgg ataatctttt actagttgtc atgcagtctg cgcatctcac aattcagaga
     2881 aaagctttcc agcagtccat tgagggactt atgactctgt gccaggagca gacatccagt
     2941 cagccggtca tcgccaaagc tttgcagcag ctgaagaatg atgcattgga gctatgcaac
     3001 aggataagca atgccattga ccgcgtggac cacatgttca catcagaatt tgatgctgag
     3061 gttgatgaat ctgaatctgt caccttgcaa cagtactacc gagaagcaat gattcagggg
     3121 tacaattttg gatttgagta tcataaagaa gttgttcgtt tgatgtctgg ggagtttaga
     3181 cagaagatag gagacaaata tataagcttt gcccggaagt ggatgaatta tgtcctgact
     3241 aaatgtgaga gtggtagagg tacaagaccc aggtgggcga ctcaaggatt tgattttcta
     3301 caagcaattg aacctgcctt tatttcagct ttaccagaag atgacttctt gagtttacaa
     3361 gccttgatga atgaatgcat tggccatgtc ataggaaaac cacacagtcc tgttacaggt
     3421 ttgtaccttg ccattcatcg gaacagcccc cgtcctatga aggtacctcg atgccatagt
     3481 gaccctccta acccacacct cattatcccc actccagagg gattcagcac tcggagcatg
     3541 ccttccgacg cgcggagcca tggcagccct gctgctgctg ctgctgctgc tgctgctgct
     3601 gttgctgcca gtcggcccag cccctctggt ggtgactctg tgctgcccaa atccatcagc
     3661 agtgcccatg ataccagggg ttccagcgtt cctgaaaatg atcgattggc ttccatagct
     3721 gctgaattgc agtttaggtc cctgagtcgt cactcaagcc ccacggagga gcgagatgaa
     3781 ccagcatatc caagaggaga ttcaagtggg tccacaagaa gaagttggga acttcggaca
     3841 ctaatcagcc agagtaaaga tactgcttct aaactaggac ccatagaagc tatccagaag
     3901 tcagtccgat tgtttgaaga aaagaggtac cgagaaatga ggagaaagaa tatcattggt
     3961 caagtttgtg atacgcctaa gtcctatgat aatgttatgc acgttggctt gaggaaggtg
     4021 accttcaaat ggcaaagagg aaacaaaatt ggagaaggcc agtatgggaa ggtgtacacc
     4081 tgcatcagcg tcgacaccgg ggagctgatg gccatgaaag agattcgatt tcaacctaat
     4141 gaccataaga ctatcaagga aactgcagac gaattgaaaa tattcgaagg catcaaacac
     4201 cccaatctgg ttcggtattt tggtgtggag ctccatagag aagaaatgta catcttcatg
     4261 gagtactgcg atgaggggac tttagaagag gtgtcaaggc tgggacttca ggaacatgtg
     4321 attaggctgt attcaaagca gatcaccatt gcgatcaacg tcctccatga gcatggcata
     4381 gtccaccgtg acattaaagg tgccaatatc ttccttacct catctggatt aatcaaactg
     4441 ggagattttg gatgttcagt aaagctcaaa aacaatgccc agaccatgcc tggtgaagtg
     4501 aacagcaccc tggggacagc agcatacatg gcacctgaag tcatcactcg tgccaaagga
     4561 gagggccatg ggcgtgcggc cgacatctgg agtctggggt gtgttgtcat agagatggtg
     4621 actggcaaga ggccttggca tgagtatgag cacaactttc aaattatgta taaagtgggg
     4681 atgggacata agccaccaat ccctgaaaga ttaagccctg aaggaaagga cttcctttct
     4741 cactgccttg agagtgaccc aaagatgaga tggaccgcca gccagctcct cgaccattcg
     4801 tttgtcaagg tttgcacaga tgaagaatga
//
