LOCUS       sequence-219634-        4827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of MAP3K4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-MAP3K4
FEATURES             Location/Qualifiers
     source          1..4827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      58..67
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             1965..1973
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgagagaag ccgctgccgc gctggtccct cctcccgcct ttgccgtcac gcctgccgcc
       61 gccatggagg agccgccgcc accgccgccg ccgccaccac cgccaccgga acccgagacc
      121 gagtcagaac ccgagtgctg cttggcggcg aggcaagagg gcacattggg agattcagct
      181 tgcaagagtc ctgaatctga tctagaagac ttctccgatg aaacaaatac agagaatctt
      241 tatggtacct ctccccccag cacacctcga cagatgaaac gcatgtcaac caaacatcag
      301 aggaataatg tggggaggcc agccagtcgg tctaatttga aagaaaaaat gaatgcacca
      361 aatcagcctc cacataaaga cactggaaaa acagtggaga atgtggaaga atacagctat
      421 aagcaggaga aaaagatccg agcagctctt agaacaacag agcgtgatca taaaaaaaat
      481 gtacagtgct cattcatgtt agactcagtg ggtggatctt tgccaaaaaa atcaattcca
      541 gatgtggatc tcaataagcc ttacctcagc cttggctgta gcaatgctaa gcttccagta
      601 tctgtgccca tgcctatagc cagacctgca cgccagactt ctaggactga ctgtccagca
      661 gatcgtttaa agttttttga aactttacga cttttgctaa agcttacctc agtctcaaag
      721 aaaaaagaca gggagcaaag aggacaagaa aatacgtctg gtttctggct taaccgatct
      781 aacgaactga tctggttaga gctacaagcc tggcatgcag gacggacaat taacgaccag
      841 gacttctttt tatatacagc ccgtcaagcc atcccagata ttattaatga aatccttact
      901 ttcaaagtcg actatgggag cttcgccttt gttagagata gagctggttt taatggtact
      961 tcagtagaag ggcagtgcaa agccactcct ggaacaaaga ttgtaggtta ctcaacacat
     1021 catgagcatc tccaacgcca gagggtctca tttgagcagg taaaacggat aatggagctg
     1081 ctagagtaca tagaagcact ttatccatca ttgcaggctc ttcagaagga ctatgaaaaa
     1141 tatgctgcaa aagacttcca ggacagggtg caggcactct gtttgtggtt aaacatcaca
     1201 aaagacttaa atcagaaatt aaggattatg ggcactgttt tgggcatcaa gaatttatca
     1261 gacattggct ggccagtgtt tgaaatccct tcccctcgac catccaaagg taatgagccg
     1321 gagtatgagg gtgatgacac agaaggagaa ttaaaggagt tggaaagtag tacggatgag
     1381 agtgaagaag aacaaatctc tgatcctagg gtaccggaaa tcagacagcc catagataac
     1441 agcttcgaca tccagtcgcg ggactgcata tccaagaagc ttgagaggct cgaatctgag
     1501 gatgattctc ttggctgggg agcaccagac tggagcacag aagcaggctt tagtagacat
     1561 tgtctgactt ctatttatag accatttgta gacaaagcac tgaagcagat ggggttaaga
     1621 aagttaattt taagacttca caagctaatg gatggttcct tgcaaagggc acgtatagca
     1681 ttggtaaaga acgatcgtcc agtggagttt tctgaatttc cagatcccat gtggggttca
     1741 gattatgtgc agttgtcaag gacaccacct tcatctgagg agaaatgcag tgctgtgtcg
     1801 tgggaggagc tgaaggccat ggatttacct tcattcgaac ctgccttcct agttctctgc
     1861 cgagtccttc tgaatgtcat acatgagtgt ctgaagttaa gattggagca gagacctgct
     1921 ggagaaccat ctctcttgag tattaagcag ctggtgagag agtgtaagga ggtcctgaag
     1981 ggcggcctgc tgatgaagca gtactaccag ttcatgctgc aggaggttct ggaggacttg
     2041 gagaagcccg actgcaacat tgacgctttt gaagaggatc tacataaaat gcttatggtg
     2101 tattttgatt acatgagaag ctggatccaa atgctacagc aattacctca agcatcgcat
     2161 agtttaaaaa atctgttaga agaagaatgg aatttcacca aagaaataac tcattacata
     2221 cggggaggag aagcacaggc cgggaagctt ttctgtgaca ttgcaggaat gctgctgaaa
     2281 tctacaggaa gttttttaga atttggctta caggagagct gtgctgaatt ttggactagt
     2341 gcggatgaca gcagtgcttc cgacgaaatc aggaggtctg ttatagagat cagtcgagcc
     2401 ctgaaggagc tcttccatga agccagagaa agggcttcca aagcacttgg atttgctaaa
     2461 atgttgagaa aggacctgga aatagcagca gaattcaggc tttcagcccc agttagagac
     2521 ctcctggatg ttctgaaatc aaaacagtat gtcaaggtgc aaattcctgg gttagaaaac
     2581 ttgcaaatgt ttgttccaga cactcttgct gaggagaaga gtattatttt gcagttactc
     2641 aatgcagctg caggaaagga ctgttcaaaa gattcagatg acgtactcat cgatgcctat
     2701 ctgcttctga ccaagcacgg tgatcgagcc cgtgattcag aggacagctg gggcacctgg
     2761 gaggcacagc ctgtcaaagt cgtgcctcag gtggagactg ttgacaccct gagaagcatg
     2821 caggtggata atcttttact agttgtcatg cagtctgcgc atctcacaat tcagagaaaa
     2881 gctttccagc agtccattga gggacttatg actctgtgcc aggagcagac atccagtcag
     2941 ccggtcatcg ccaaagcttt gcagcagctg aagaatgatg cattggagct atgcaacagg
     3001 ataagcaatg ccattgaccg cgtggaccac atgttcacat cagaatttga tgctgaggtt
     3061 gatgaatctg aatctgtcac cttgcaacag tactaccgag aagcaatgat tcaggggtac
     3121 aattttggat ttgagtatca taaagaagtt gttcgtttga tgtctgggga gtttagacag
     3181 aagataggag acaaatatat aagctttgcc cggaagtgga tgaattatgt cctgactaaa
     3241 tgtgagagtg gtagaggtac aagacccagg tgggcgactc aaggatttga ttttctacaa
     3301 gcaattgaac ctgcctttat ttcagcttta ccagaagatg acttcttgag tttacaagcc
     3361 ttgatgaatg aatgcattgg ccatgtcata ggaaaaccac acagtcctgt tacaggtttg
     3421 taccttgcca ttcatcggaa cagcccccgt cctatgaagg tacctcgatg ccatagtgac
     3481 cctcctaacc cacacctcat tatccccact ccagagggat tcagcactcg gagcatgcct
     3541 tccgacgcgc ggagccatgg cagccctgct gctgctgctg ctgctgctgc tgctgctgtt
     3601 gctgccagtc ggcccagccc ctctggtggt gactctgtgc tgcccaaatc catcagcagt
     3661 gcccatgata ccaggggttc cagcgttcct gaaaatgatc gattggcttc catagctgct
     3721 gaattgcagt ttaggtccct gagtcgtcac tcaagcccca cggaggagcg agatgaacca
     3781 gcatatccaa gaggagattc aagtgggtcc acaagaagaa gttgggaact tcggacacta
     3841 atcagccaga gtaaagatac tgcttctaaa ctaggaccca tagaagctat ccagaagtca
     3901 gtccgattgt ttgaagaaaa gaggtaccga gaaatgagga gaaagaatat cattggtcaa
     3961 gtttgtgata cgcctaagtc ctatgataat gttatgcacg ttggcttgag gaaggtgacc
     4021 ttcaaatggc aaagaggaaa caaaattgga gaaggccagt atgggaaggt gtacacctgc
     4081 atcagcgtcg acaccgggga gctgatggcc atgaaagaga ttcgatttca acctaatgac
     4141 cataagacta tcaaggaaac tgcagacgaa ttgaaaatat tcgaaggcat caaacacccc
     4201 aatctggttc ggtattttgg tgtggagctc catagagaag aaatgtacat cttcatggag
     4261 tactgcgatg aggggacttt agaagaggtg tcaaggctgg gacttcagga acatgtgatt
     4321 aggctgtatt caaagcagat caccattgcg atcaacgtcc tccatgagca tggcatagtc
     4381 caccgtgaca ttaaaggtgc caatatcttc cttacctcat ctggattaat caaactggga
     4441 gattttggat gttcagtaaa gctcaaaaac aatgcccaga ccatgcctgg tgaagtgaac
     4501 agcaccctgg ggacagcagc atacatggca cctgaagtca tcactcgtgc caaaggagag
     4561 ggccatgggc gtgcggccga catctggagt ctggggtgtg ttgtcataga gatggtgact
     4621 ggcaagaggc cttggcatga gtatgagcac aactttcaaa ttatgtataa agtggggatg
     4681 ggacataagc caccaatccc tgaaagatta agccctgaag gaaaggactt cctttctcac
     4741 tgccttgaga gtgacccaaa gatgagatgg accgccagcc agctcctcga ccattcgttt
     4801 gtcaaggttt gcacagatga agaatga
//
