LOCUS       sequence-21966-e         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Vander Haar E, Lee SI, Bandhakavi S, Griffin TJ, Kim DH
  TITLE     Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40.
  JOURNAL   Nat Cell Biol. 2007 Mar . 9(3):316-23.
  PUBMED    17277771
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2007 Mar . 9(3):316-23."
COMMENT     Sequence Label: pRK5-myc-PRAS40
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      87..96
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             96..125
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gtgtccactc ccaggtccaa ctgcacctcg gttctatcga ttgaattccc gaaccgacag
       61 tcggtctctt caccaaggcc attcgcgcca ccatggagca aaagctcatt tctgaggaag
      121 atctcaatgg tggtggtggt gggtcgacga tggcgtcggg tcgccctgag gagctgtggg
      181 aggccgtggt gggggccgct gagcgcttcc gggcccggac tggcacggag ctggtgctgc
      241 tgaccgcggc cccgccgcca ccaccccgcc cgggcccctg tgcctatcct gcccatggtc
      301 gaggagccct ggcggaggca gcgcgccgtt gcctccacga catcgcactg gcccacaggg
      361 ctgccactgc tgctcggcct cctgcgcccc caccagcacc acagccaccc agtcccacac
      421 ccagcccacc ccggcctacc ctggccagag aggacaacga ggaggacgag gatgagccca
      481 cagagacaga gacctccggg gagcagctgg gcattagtga taatggaggg ctctttgtga
      541 tggatgagga cgccaccctc caggaccttc cccccttctg tgagtcagac cccgagagta
      601 cagatgatgg cagcctgagc gaggagaccc ccgccggccc tcccacctgc tcagtgcccc
      661 cagcct
//
