LOCUS       sequence-21967-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Vander Haar E, Lee SI, Bandhakavi S, Griffin TJ, Kim DH
  TITLE     Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40.
  JOURNAL   Nat Cell Biol. 2007 Mar . 9(3):316-23.
  PUBMED    17277771
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2007 Mar . 9(3):316-23."
COMMENT     Sequence Label: pLKO.1-PRAS40
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    108..225
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        274..784
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     703..721
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(805..824)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 acgaaacacc ggtcagctgg gcattagtga taatctcgag attatcacta atgcccagct
       61 gtttttgaat tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa
      121 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      181 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
      241 gacagcagag atccactttg gccgcggctc gagggggttg gggttgcgcc ttttccaagg
      301 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
      361 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
      421 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
      481 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
      541 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
      601 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
      661 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa
      721 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc
      781 ccaggggatc caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
      841 cgcgacgacg tccccaaggg gcgtacgcac cctcgccgcc gcgttcgccg actacccggc
      901 c
//
