LOCUS       sequence-21968-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Vander Haar E, Lee SI, Bandhakavi S, Griffin TJ, Kim DH
  TITLE     Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40.
  JOURNAL   Nat Cell Biol. 2007 Mar . 9(3):316-23.
  PUBMED    17277771
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2007 Mar . 9(3):316-23."
COMMENT     Sequence Label: pLKO.1-TSC2
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    104..221
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        270..780
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     699..717
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(801..820)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 aacaccggtc actggccttg gacggtattg ctcgagcaat accgtccaag gccagtgttt
       61 ttgaattctc gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg
      121 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
      181 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
      241 gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc
      301 cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc
      361 gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct
      421 acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg
      481 cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca
      541 gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc
      601 ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg
      661 tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct
      721 ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctcccagg
      781 gggatccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
      841 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgacta
//
