LOCUS       sequence-219682-        9180 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of AKAP13__RET fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 9180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-AKAP13__RET
FEATURES             Location/Qualifiers
     source          1..9180
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(8071..8095)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgaaactta atccacagca agctccctta tatggtgatt gtgttgttac agtgctgctt
       61 gctgaagagg acaaagctga agatgatgta gtgttttact tggtattttt gggttccacc
      121 ctccgtcact gtacaagtac tcggaaggtc agttctgata cattggagac cattgctcct
      181 ggtcatgatt gttgtgaaac agtgaaggtg cagctctgtg cttccaaaga gggccttccc
      241 gtgtttgtgg tggctgaaga agactttcat ttcgtccagg atgaagcgta tgatgcagct
      301 caattcctag caaccagtgc tggaaatcag caggctttga actttacccg ttttcttgac
      361 cagtcaggac ccccatctgg ggatgtgaat tcccttgata agaagttggt gctggcattc
      421 aggcacctga agctgcccac ggagtggaat gtattgggga cagatcagag tttgcatgat
      481 gctggcccgc gagagacatt gatgcatttt gctgtgcggc tgggactgct gaggttgacg
      541 tggttcctgt tgcagaagcc aggtggccgc ggagctctca gtatccacaa ccaggaaggg
      601 gcgacgcctg tgagcttggc cttggagcga ggctatcaca agctgcacca gcttctaacc
      661 gaggagaatg ctggagaacc agactcctgg agcagtttat cctatgaaat accgtatgga
      721 gactgttctg tgaggcatca tcgagagttg gacatctata cattaacctc tgagtctgat
      781 tcacatcatg aacacccatt tcctggagac ggttgcactg gaccaatttt taaacttatg
      841 aacatccaac agcaactaat gaaaacaaac ctcaagcaga tggacagtct tatgccctta
      901 atgatgacag cacaggatcc ttccagtgcc ccagagacag atggccagtt tcttccctgt
      961 gcaccggagc ccacggaccc tcagcgactt tcttcttctg aagagactga gagcactcag
     1021 tgctgcccag ggagccctgt tgcacagact gaaagtccct gtgatttgtc aagcatagtt
     1081 gaggaggaga atacagaccg ttcctgtagg aagaaaaata aaggcgtgga aagaaaaggg
     1141 gaagaggtgg agccagcacc tattgtggac tctggaactg tatctgatca agacagctgc
     1201 cttcagagct tgcctgattg tggagtaaag ggcacggaag gcctttcgtc ctgtggaaac
     1261 agaaatgaag aaactggaac aaaatcttct ggaatgccca cagaccagga gtccctgagc
     1321 agtggagatg ctgtgcttca gagagacttg gtcatggagc caggcacagc ccagtattcc
     1381 tctggaggtg aactgggagg catttcaaca acaaatgtca gtaccccaga cactgcaggg
     1441 gaaatggaac atgggctcat gaacccagat gccactgttt ggaagaatgt gcttcaggga
     1501 ggggaaagta caaaggaaag atttgagaac tctaatattg gcacagctgg agcctctgac
     1561 gtgcacgtca caagtaagcc tgtggataaa atcagtgttc caaactgtgc ccctgctgcc
     1621 agttccctgg atggtaacaa acctgctgag tcttcacttg catttagtaa tgaagaaacc
     1681 tccactgaaa aaacagcaga aacggaaact tcacgaagtc gtgaggagag tgctgatgct
     1741 ccagtagatc agaattctgt ggtgattcca gctgctgcaa aagacaagat ttcagatgga
     1801 ttagaacctt atactctctt agcagcaggc ataggtgagg caatgtcacc ctcagattta
     1861 gcccttcttg ggctggaaga agatgtaatg ccacaccaga actcagaaac aaattcatct
     1921 catgctcaaa gccaaaaggg caaatcctca cccatttgtt ctacaactgg agacgataaa
     1981 ctttgtgcag actctgcatg tcaacagaac acagtgactt ctagtggcga tttggttgca
     2041 aaactgtgtg ataacatagt tagcgagtcc gaaagcacca cagcaaggca acccagctca
     2101 caagatccac ccgatgcctc ccactgtgaa gacccacagg ctcatacagt cacctctgac
     2161 cctgtaaggg atacccagga acgtgcggat ttttgtcctt tcaaagtggt ggataacaaa
     2221 ggccaacgaa aagatgtgaa actagataaa cctttaacaa atatgcttga ggtggtttca
     2281 catccacatc cagttgtccc taaaatggag aaagaactgg tgccagacca ggcagtaata
     2341 tcagacagta ctttctctct ggcaaacagt ccaggcagtg aatcagtaac caaggatgac
     2401 gcactttctt ttgtcccctc ccagaaagaa aagggaacag caactcctga actacataca
     2461 gctacagatt atagagatgg cccagatgga aattcgaatg agcctgatac gcggccacta
     2521 gaagacaggg cagtaggcct gtccacatcc tccactgctg cagagcttca gcacgggatg
     2581 gggaatacca gtctcacagg acttggtgga gagcatgagg gtcccgcccc tccagcaatc
     2641 ccagaagctc tgaatatcaa ggggaacact gactcttccc tgcaaagtgt gggtaaggcc
     2701 actttggctt tagattcagt tttgactgaa gaaggaaaac ttctggtggt ttcagaaagc
     2761 tctgcagctc aggaacaaga taaggataaa gcggtgacct gttcctctat taaggaaaat
     2821 gctctctctt caggaacttt gcaggaagag cagagaacac cacctcctgg acaagatact
     2881 caacaatttc atgaaaaatc aatctcagct gactgtgcca aggacaaagc acttcagcta
     2941 agtaattcac cgggtgcatc ctctgccttt cttaaggcag aaactgaaca taacaaggaa
     3001 gtggccccac aagtctcact gctgactcaa ggtggggctg cccagagcct ggtgccacca
     3061 ggagcaagtc tggccacaga gtcaaggcag gaagccttgg gggcagagca caacagctcc
     3121 gctctgttgc catgtctgtt gccagatggg tctgatgggt ccgatgctct taactgcagt
     3181 cagccttctc ctctggatgt tggagtgaag aacactcaat cccagggaaa aactagtgcc
     3241 tgtgaggtga gtggagatgt gacggtggat gttacagggg ttaatgctct acaaggtatg
     3301 gctgagccca gaagagagaa tatatcacac aacacccaag acatcctgat tccaaacgtc
     3361 ttgttgagcc aagagaagaa tgccgttcta ggtttgccag tggctctaca ggacaaagct
     3421 gtgactgacc cacagggagt tggaacccca gagatgatac ctcttgattg ggagaaaggg
     3481 aagctggagg gagcagacca cagctgtacc atgggtgacg ctgaggaagc ccaaatagac
     3541 gatgaagcac atcctgtcct actgcagcct gttgccaagg agctccccac agacatggag
     3601 ctctcagccc atgatgatgg ggccccagct ggtgtgaggg aagtcatgcg agccccgcct
     3661 tcaggcaggg aaaggagcac tccctctcta ccttgcatgg tctctgccca ggacgcacct
     3721 ctgcctaagg gggcagactt gatagaggag gctgccagcc gtatagtgga tgctgtcatc
     3781 gaacaagtca aggccgctgg agcactgctt actgaggggg aggcctgtca catgtcactg
     3841 tccagccctg agttgggtcc tctcactaaa ggactagaga gtgcttttac agaaaaagtg
     3901 agtactttcc cacctgggga gagcctacca atgggcagta ctcctgagga agccacgggg
     3961 agccttgcag gatgttttgc tggaagggag gagccagaga agatcatttt acctgtccag
     4021 gggcctgagc cagcagcaga aatgccagac gtgaaagctg aagatgaagt ggattttaga
     4081 gcaagttcaa tttctgaaga agtggctgta gggagcatag ctgctacact gaagatgaag
     4141 caaggcccaa tgacccaggc gataaaccga gaaaactggt gtacaataga gccatgccct
     4201 gatgcagcat ctcttctggc ttccaagcag agcccagaat gtgagaactt cctggatgtt
     4261 ggactgggca gagagtgtac ctcaaaacaa ggtgtactta aaagagaatc tgggagtgat
     4321 tctgacctct ttcactcacc cagtgatgac atggacagca tcatcttccc aaagccagag
     4381 gaagagcatt tggcctgtga tatcaccgga tccagttcat ccaccgatga cacggcttca
     4441 ctggaccgac attcttctca tggcagtgat gtgtctctct cccagatttt aaagccaaac
     4501 aggtcaagag atcggcaaag ccttgatgga ttctacagcc atgggatggg agctgagggt
     4561 cgagaaagtg agagtgagcc tgctgaccca ggcgacgtgg aggaggagga gatggacagt
     4621 atcactgaag tgcctgcaaa ctgctctgtc ctaaggagct ccatgcgctc tctttctccc
     4681 ttccggaggc acagctgggg gcctgggaaa aatgcagcca gcgatgcaga aatgaaccac
     4741 cggagtatga gctggtgccc ctctggtgtg cagtactctg ctggcctgag tgctgacttt
     4801 aattacagaa gtttcagtct agaaggcttg acaggaggag ctggtgtcgg aaacaagcca
     4861 tcctcatctc tagaagtaag ctctgcaaat gccgaagagc tcagacaccc attcagtggt
     4921 gaggaacggg ttgactcttt ggtgtcactt tcagaagagg atctggagtc agaccagaga
     4981 gaacatagga tgtttgatca gcagatatgt cacagatcta agcagcaggg atttaattac
     5041 tgtacatcag ccatttcctc tccattgaca aaatccatct cattaatgac aatcagccat
     5101 cctggattgg acaattcacg gcccttccac agtaccttcc acaataccag tgctaatctg
     5161 actgagagta taacagaaga gaactataat ttcctgccac atagcccctc caagaaagat
     5221 tctgaatgga agagtggaac aaaagtcagt cgtacattca gctacatcaa gaataaaatg
     5281 tctagcagca agaagagcaa agaaaaggaa aaagaaaaag ataagattaa ggagaaggag
     5341 aaagattcta aagacaagga gaaagataag aagactgtca acgggcacac tttcagttcc
     5401 attcctgttg tgggtcccat cagctgtagc cagtgtatga agcccttcac caacaaagat
     5461 gcctatactt gtgcaaattg cagtgctttt gtccacaaag gctgccgaga aagtctagcc
     5521 tcctgtgcaa aggtcaaaat gaagcagccc aaagggagcc ttcaggcaca tgacacatca
     5581 tcactgccca cggtcattat gagaaacaag ccctcacagc ccaaggagcg tcctcggtcc
     5641 gcagtcctcc tggtggatga aaccgctacc accccaatat ttgccaatag acgatcccag
     5701 cagagtgtct cgctctccaa aagtgtctcc atacagaaca ttactggagt tggcaatgat
     5761 gagaacatgt caaacacctg gaaattcctg tctcattcaa cagactcact aaataaaatc
     5821 agcaaggtca atgagtcaac agaatcactt actgatgagg gagtaggtac agacatgaat
     5881 gaaggacaac tactgggaga ctttgagatt gagtccaaac agctggaagc agagtcttgg
     5941 agtcggataa tagacagcaa gtttctaaaa cagcaaaaga aagatgtggt caaacggcaa
     6001 gaagtaatat atgagttgat gcagacagag tttcatcatg tccgcactct caagatcatg
     6061 agtggtgtgt acagccaggg gatgatggcg gatctgcttt ttgagcagca gatggtagaa
     6121 aagctgttcc cctgtttgga tgagctgatc agtatccata gccaattctt ccagaggatt
     6181 ctggagcgga agaaggagtc tctggtggat aaaagtgaaa agaactttct catcaagagg
     6241 ataggggatg tgcttgtaaa tcagttttca ggtgagaatg cagaacgttt aaagaagaca
     6301 tatggcaagt tttgtgggca acataaccag tctgtaaact acttcaaaga cctttatgcc
     6361 aaggataagc gttttcaagc ctttgtaaag aagaagatga gcagttcagt tgttagaagg
     6421 cttggaattc cagagtgcat attgcttgta actcagcgga ttaccaagta cccagtttta
     6481 ttccaaagaa tattgcagtg taccaaagac aatgaagtgg agcaggaaga tctagcacag
     6541 tccttgagcc tggtgaagga tgtgattgga gctgtagaca gcaaagtggc aagttatgaa
     6601 aagaaagtgc gtctcaatga gatttataca aagacagata gcaagtcaat catgaggatg
     6661 aagagtggtc agatgtttgc caaggaagat ttgaaacgga agaagcttgt acgtgatggg
     6721 agtgtgtttc tgaagaatgc agcaggaagg ttgaaagagg ttcaagcagt tcttctcact
     6781 gacattttag ttttccttca agaaaaagac cagaagtaca tctttgcatc attggaccag
     6841 aagtcaacag tgatctcttt aaagaagctg attgtgagag aagtggcaca tgaggagaaa
     6901 ggtttattcc tgatcagcat ggggatgaca gatccagaga tggtagaagt ccatgccagc
     6961 tccaaagagg aacgaaacag ctggattcag atcattcagg acacaatcaa caccctgaac
     7021 agagatgaag atgaaggaat tcctagtgag aatgaggaag aaaagaaaat gttggacacc
     7081 agagcccgag aattaaaaga acaacttcac cagaaggacc aaaaaatcct actcttgttg
     7141 gaagagaagg agatgatttt ccgggacatg gctgagtgca gcacccctct cccagaggat
     7201 tgctccccaa cacatagccc tagagttctc ttccgctcca acacagaaga ggctctcaaa
     7261 ggaggacctt taatgaaaag tgcaataaat gaggtggaga tccttcaggg tttggtgagt
     7321 ggaaatctgg gaggcacact tgggccgact gtcagcagcc ccattgagca agatgtggtc
     7381 ggtcccgttt ccctgccccg gagagcagag acctttggag gatttgacag ccatcagatg
     7441 aatgcttcaa aaggaggcga gaaggaagag ggagatgatg gccaagatct taggagaacg
     7501 gaatcagata gtggcctaaa aaagggtgga aatgctaacc tggtatttat gcttaaaaga
     7561 aacagtgagc aggttgtcca gagcgttgtt catctctacg agctcctcag cgctctgcag
     7621 ggtgtggtgc tgcagcagga cagctacatt gaggaccaga aactggtgct gagcgagagg
     7681 gcgctcactc gcagcttgtc ccgcccgagc tccctcattg agcaggagaa gcagcgcagc
     7741 ctggagaagc agcgccagga cctggccaac ctgcagaagc agcaggccca gtacctcgag
     7801 gagaagcgca ggcgcgagcg tgagtgggaa gctcgtgaga gggagctgcg ggagcgggag
     7861 gccctcctgg cccagcgcga ggaggaggtg cagcaggggc agcaggacct ggaaaaggag
     7921 cgggaggagc tccagcagaa gaagggcaca taccagtatg acctggagcg actgcgtgct
     7981 gcccagaaac agcttgagag ggaacaggag cagctgcgcc gggaggcaga gcggctcagc
     8041 cagcggcaga cagaacggga cctgtgtcag tcccagcttt cttgtacaaa gtggttcgag
     8101 gatccaaagt gggaattccc tcggaagaac ttggttcttg gaaaaactct aggagaaggc
     8161 gaatttggaa aagtggtcaa ggcaacggcc ttccatctga aaggcagagc agggtacacc
     8221 acggtggccg tgaagatgct gaaagagaac gcctccccga gtgagcttcg agacctgctg
     8281 tcagagttca acgtcctgaa gcaggtcaac cacccacatg tcatcaaatt gtatggggcc
     8341 tgcagccagg atggcccgct cctcctcatc gtggagtacg ccaaatacgg ctccctgcgg
     8401 ggcttcctcc gcgagagccg caaagtgggg cctggctacc tgggcagtgg aggcagccgc
     8461 aactccagct ccctggacca cccggatgag cgggccctca ccatgggcga cctcatctca
     8521 tttgcctggc agatctcaca ggggatgcag tatctggccg agatgaagct cgttcatcgg
     8581 gacttggcag ccagaaacat cctggtagct gaggggcgga agatgaagat ttcggatttc
     8641 ggcttgtccc gagatgttta tgaagaggat tcgtacgtga agaggagcca gggtcggatt
     8701 ccagttaaat ggatggcaat tgaatccctt tttgatcata tctacaccac gcaaagtgat
     8761 gtatggtctt ttggtgtcct gctgtgggag atcgtgaccc tagggggaaa cccctatcct
     8821 gggattcctc ctgagcggct cttcaacctt ctgaagaccg gccaccggat ggagaggcca
     8881 gacaactgca gcgaggagat gtactgcctg atgctgcaat gctggaagca ggagccggac
     8941 aaaaggccgg tgtttgcgga catcagcaaa gacctggaga agatgatggt taagaggaga
     9001 gactacttgg accttgcggc gtccactcca tctgactccc tgatttatga cgacggcctc
     9061 tcagaggagg agacaccgct ggtggactgt aataatgccc ccctccctcg agccctccct
     9121 tccacatgga ttgaaaacaa actctatggt agaatttccc atgcatttac tagattctag
//
