LOCUS       sequence-21969-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Vander Haar E, Lee SI, Bandhakavi S, Griffin TJ, Kim DH
  TITLE     Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40.
  JOURNAL   Nat Cell Biol. 2007 Mar . 9(3):316-23.
  PUBMED    17277771
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2007 Mar . 9(3):316-23."
COMMENT     Sequence Label: pRK5-myc-PRAS40 T246A
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             113..142
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tattngcttt tttcnaggtg tccactccca ggtccaactg cacctcggtt ctatcgattg
       61 aattcccgaa ccnanagtcg gtctcttcac caaggccatt cgcgccacca tggagcaaaa
      121 gctcatttct gaggaagatc tcaatggtgg tggtggtggg tcgaccatgg cgtcgggtcg
      181 ccctgaggag ctgtgggagg ccgtggtggg ggccgctgag cgcttccggg cccggactgg
      241 cacggagctg gtgctgctga ccgcggcccc gccgccacca ccccgcccgg gcccctgtgc
      301 ctatcctgcc catggtcgag gagccctggc ggaggcagcg cgccgttgcc tccacgacat
      361 cgcactggcc cacagggctg ccactgctgc tcggcctcct gcgcccccac cagcaccaca
      421 gccacccagt cccacaccca gcccatcccg gcctaccctg gccagagagg acaacgagga
      481 ggacgaggat gagcccacag agacagagac ctccggggag cagctgggca ttagtgataa
      541 tggagggctc tttgtgatgg atgaggacgc caccctccag gaccttcccc ccttctgtga
      601 gtcagacccc gagagtacag atgatggcag cctgagcgag gagacccccg ccggcccccc
      661 cangtgttag tggccccaan cnttcagccc taccntngga gcagtacgcc aagtccctgc
      721 ctgtgtctgt gcccgtctgg gg
//
