LOCUS       sequence-21970-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Vander Haar E, Lee SI, Bandhakavi S, Griffin TJ, Kim DH
  TITLE     Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40.
  JOURNAL   Nat Cell Biol. 2007 Mar . 9(3):316-23.
  PUBMED    17277771
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2007 Mar . 9(3):316-23."
COMMENT     Sequence Label: pLKO.1-mPRAS40
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    105..222
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        271..781
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     700..718
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(804..823)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 aaacaccggt gagcccactg aaacagagac actcgagtgt ctctgtttca gtgggctctt
       61 tttgaattct cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
      121 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
      181 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
      241 agcagagatc cactttggcc gcggctcgag ggggttgggg ttgcgccttt tccaaggcag
      301 ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc
      361 cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc
      421 tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt
      481 gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc
      541 agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag
      601 cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg
      661 gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc
      721 tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca
      781 ggggggatcc accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
      841 gcgacgacgt ccccaggggc gtacgcaccc tcgccgccg
//
