LOCUS       sequence-219786-       13948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination vector encoding Maize ubiquitin promoter driving
            Cas9 and 35S promoter driving Zeocin for selection in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13948)
  AUTHORS   Mallett DR, Chang M, Cheng X, Bezanilla M
  TITLE     Efficient and modular CRISPR-Cas9 vector system for Physcomitrella 
            patens.
  JOURNAL   Plant Direct. 2019 Sep 12;3(9):e00168. doi: 10.1002/pld3.168. 
            eCollection 2019 Sep.
  PUBMED    31523744
REFERENCE   2  (bases 1 to 13948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2019-09-12"; volume: "3(9):e00168. doi"; pages: " 
            10.1002/pld3.168. eCollection 2019 Sep"
COMMENT     Sequence Label: pZeo-Cas9-gate
FEATURES             Location/Qualifiers
     source          1..13948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1009..1027
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1009..1026
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    1106..1230
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1255..1260,1261..1278,1279..1285)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1339..1998
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1405..1424)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2340..2645
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2524..2543
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2686..2810)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2833..4824
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    4847..4871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4898..4918
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4919..9022
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4919..9019
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    complement(9039..9063)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      9076..9085
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        9483..9662
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    9782..9815
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     10009..10025
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10009..10025
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    10537..10713
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10783..11157)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVLVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(11161..11506)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(11198..11218)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    11992..12025
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      12054..12063
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      12058..12067
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(12097..12116)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12098..12116)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12123..12140)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12123..12139)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12132..12154)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12280..12735
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12367..12386)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12577..12598
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12813..12917
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12918..12986,12987..13778)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13136..13155)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat ttaggtgaca
     1021 ctatagaata ctcaagctat gcatccaacg cgttgggaaa ctgaaggcgg gaaacgacaa
     1081 tctgatccaa gctcaagcta agcttacaag tttgtacaaa aaagctgaac gagaaacgta
     1141 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     1201 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca
     1261 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
     1321 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     1381 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     1441 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     1501 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
     1561 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     1621 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     1681 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     1741 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     1801 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     1861 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     1921 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     1981 tggcagggcg gggcgtaaac gcgtggatcc ggcttactaa aagccagata acagtatgcg
     2041 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     2101 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     2161 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     2221 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     2281 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
     2341 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     2401 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     2461 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     2521 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     2581 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     2641 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
     2701 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     2761 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt tctagagatc
     2821 aagcttgcat gcctgcagtg cagcgtgacc cggtcgtgcc cctctctaga gataatgagc
     2881 attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact tgtttgaagt
     2941 gcagtttatc tatctttata catatattta aactttactc tacgaataat ataatctata
     3001 gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag acatggtcta
     3061 aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta gtgtgcatgt
     3121 gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat tttattagta
     3181 catccattta gggtttaggg ttaatggttt ttatagacta atttttttag tacatctatt
     3241 ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt tttttattta
     3301 ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa atacccttta
     3361 agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg ccagcctgtt
     3421 aaacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc
     3481 aagcgaagca gacggcacgg catctctgtc gctgcctctg gacccctctc gagagttccg
     3541 ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac
     3601 gtgagccggc acggcaggcg gcctcctcct cctctcacgg caccggcagc tacgggggat
     3661 tcctttccca ccgctccttc gctttccctt cctcgcccgc cgtaataaat agacaccccc
     3721 tccacaccct ctttccccaa cctcgtgttg ttcggagcgc acacacacac aaccagatct
     3781 cccccaaatc cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcc tccccccccc
     3841 ccctctctac cttctctaga tcggcgttcc ggtccatggt tagggcccgg tagttctact
     3901 tctgttcatg tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac
     3961 acggatgcga cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg
     4021 gggaatcctg ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg
     4081 tttcgttgca tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg
     4141 tttgtcgggt catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg
     4201 ggcggtcgtt ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt
     4261 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     4321 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     4381 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct
     4441 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     4501 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     4561 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     4621 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     4681 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     4741 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     4801 tgttgtttgg tgttacttct gcaggtcgac tctagaggat cccggggcaa gtttgtacaa
     4861 aaaagcaggc tccgcggccg cccccttcac catggcccca aagaagaagc gcaaggtcga
     4921 caagaagtac tccatcggcc tcgacatcgg caccaattct gttggctggg ccgtgatcac
     4981 cgacgagtac aaggtgccgt ccaagaagtt caaggtcctc ggcaacaccg accgccactc
     5041 catcaagaag aatctcatcg gcgccctgct gttcgactct ggcgagacag ccgaggctac
     5101 aaggctcaag aggaccgcta gacgcaggta caccaggcgc aagaaccgca tctgctacct
     5161 ccaagagatc ttctccaacg agatggccaa ggtggacgac agcttcttcc acaggctcga
     5221 ggagagcttc ctcgtcgagg aggacaagaa gcacgagcgc catccgatct tcggcaacat
     5281 cgtggatgag gtggcctacc acgagaagta cccgaccatc taccacctcc gcaagaagct
     5341 cgtcgactcc accgataagg ccgacctcag gctcatctac ctcgccctcg cccacatgat
     5401 caagttcagg ggccacttcc tcatcgaggg cgacctcaac ccggacaact ccgatgtgga
     5461 caagctgttc atccagctcg tgcagaccta caaccagctg ttcgaggaga acccgatcaa
     5521 cgcctctggc gttgacgcca aggctattct ctctgccagg ctctctaagt cccgcaggct
     5581 cgagaatctg atcgcccaac ttccgggcga gaagaagaat ggcctcttcg gcaacctgat
     5641 cgccctctct cttggcctca ccccgaactt caagtccaac ttcgacctcg ccgaggacgc
     5701 caagctccag ctttccaagg acacctacga cgacgacctc gacaatctcc tcgcccagat
     5761 tggcgatcag tacgccgatc tgttcctcgc cgccaagaat ctctccgacg ccatcctcct
     5821 cagcgacatc ctcagggtga acaccgagat caccaaggcc ccactctccg cctccatgat
     5881 caagaggtac gacgagcacc accaggacct cacactcctc aaggccctcg tgagacagca
     5941 gctcccagag aagtacaagg agatcttctt cgaccagtcc aagaacggct acgccggcta
     6001 catcgatggc ggcgcttctc aagaggagtt ctacaagttc atcaagccga tcctcgagaa
     6061 gatggacggc accgaggagc tgctcgtgaa gctcaataga gaggacctcc tccgcaagca
     6121 gcgcaccttc gataatggct ccatcccgca ccagatccac ctcggcgagc ttcatgctat
     6181 cctccgcagg caagaggact tctacccgtt cctcaaggac aaccgcgaga agattgagaa
     6241 gatcctcacc ttccgcatcc cgtactacgt gggcccgctc gccaggggca actccaggtt
     6301 cgcctggatg accagaaagt ccgaggagac aatcaccccc tggaacttcg aggaggtggt
     6361 ggataagggc gcctctgccc agtctttcat cgagcgcatg accaacttcg acaagaacct
     6421 cccgaacgag aaggtgctcc cgaagcactc actcctctac gagtacttca ccgtgtacaa
     6481 cgagctgacc aaggtgaagt acgtgaccga ggggatgagg aagccagctt tccttagcgg
     6541 cgagcaaaag aaggccatcg tcgacctgct gttcaagacc aaccgcaagg tgaccgtgaa
     6601 gcagctcaag gaggactact tcaagaaaat cgagtgcttc gactccgtcg agatctccgg
     6661 cgtcgaggat aggttcaatg cctccctcgg gacctaccac gacctcctca agattatcaa
     6721 ggacaaggac ttcctcgaca acgaggagaa cgaggacatc ctcgaggaca tcgtgctcac
     6781 cctcaccctc ttcgaggacc gcgagatgat cgaggagcgc ctcaagacat acgcccacct
     6841 cttcgacgac aaggtgatga agcagctgaa gcgcaggcgc tataccggct ggggcaggct
     6901 ctctaggaag ctcatcaacg gcatccgcga caagcagtcc ggcaagacga tcctcgactt
     6961 cctcaagtcc gacggcttcg ccaaccgcaa cttcatgcag ctcatccacg acgactccct
     7021 caccttcaag gaggacatcc aaaaggccca ggtgtccggc caaggcgatt ccctccatga
     7081 acatatcgcc aatctcgccg gctccccggc tatcaagaag ggcattctcc agaccgtgaa
     7141 ggtggtggac gagctggtga aggtgatggg caggcacaag ccagagaaca tcgtgatcga
     7201 gatggcccgc gagaaccaga ccacacagaa gggccaaaag aactcccgcg agcgcatgaa
     7261 gaggatcgag gagggcatta aggagctggg ctcccagatc ctcaaggagc acccagtcga
     7321 gaacacccag ctccagaacg agaagctcta cctctactac ctccagaacg gccgcgacat
     7381 gtacgtggac caagagctgg acatcaaccg cctctccgac tacgacgtgg accatattgt
     7441 gccgcagtcc ttcctgaagg acgactccat cgacaacaag gtgctcaccc gctccgacaa
     7501 gaacaggggc aagtccgata acgtgccgtc cgaagaggtc gtcaagaaga tgaagaacta
     7561 ctggcgccag ctcctcaacg ccaagctcat cacccagagg aagttcgaca acctcaccaa
     7621 ggccgagaga ggcggccttt ccgagcttga taaggccggc ttcatcaagc gccagctcgt
     7681 cgagacacgc cagatcacaa agcacgtggc ccagatcctc gactcccgca tgaacaccaa
     7741 gtacgacgag aacgacaagc tcatccgcga ggtgaaggtc atcaccctca agtccaagct
     7801 cgtgtccgac ttccgcaagg acttccagtt ctacaaggtg cgcgagatca acaactacca
     7861 ccacgcccac gacgcctacc tcaatgccgt ggtgggcaca gccctcatca agaagtaccc
     7921 aaagctcgag tccgagttcg tgtacggcga ctacaaggtg tacgacgtgc gcaagatgat
     7981 cgccaagtcc gagcaagaga tcggcaaggc gaccgccaag tacttcttct actccaacat
     8041 catgaatttc ttcaagaccg agatcacgct cgccaacggc gagattagga agaggccgct
     8101 catcgagaca aacggcgaga caggcgagat cgtgtgggac aagggcaggg atttcgccac
     8161 agtgcgcaag gtgctctcca tgccgcaagt gaacatcgtg aagaagaccg aggttcagac
     8221 cggcggcttc tccaaggagt ccatcctccc aaagcgcaac tccgacaagc tgatcgcccg
     8281 caagaaggac tgggacccga agaagtatgg cggcttcgat tctccgaccg tggcctactc
     8341 tgtgctcgtg gttgccaagg tcgagaaggg caagagcaag aagctcaagt ccgtcaagga
     8401 gctgctgggc atcacgatca tggagcgcag cagcttcgag aagaacccaa tcgacttcct
     8461 cgaggccaag ggctacaagg aggtgaagaa ggacctcatc atcaagctcc cgaagtacag
     8521 cctcttcgag cttgagaacg gccgcaagag aatgctcgcc tctgctggcg agcttcagaa
     8581 gggcaacgag cttgctctcc cgtccaagta cgtgaacttc ctctacctcg cctcccacta
     8641 cgagaagctc aagggctccc cagaggacaa cgagcaaaag cagctgttcg tcgagcagca
     8701 caagcactac ctcgacgaga tcatcgagca gatctccgag ttctccaagc gcgtgatcct
     8761 cgccgatgcc aacctcgata aggtgctcag cgcctacaac aagcaccgcg ataagccaat
     8821 tcgcgagcag gccgagaaca tcatccacct cttcaccctc accaacctcg gcgctccagc
     8881 cgccttcaag tacttcgaca ccaccatcga ccgcaagcgc tacacctcta ccaaggaggt
     8941 tctcgacgcc accctcatcc accagtctat cacaggcctc tacgagacac gcatcgacct
     9001 ctcacaactc ggcggcgatt gaaagggtgg gcgcgccgac ccagctttct tgtacaaagt
     9061 ggtgatatcc cgcggccatg gcggccggga gcatgcgacg tcgatctaac tgactagccg
     9121 cggccatgct agagtccgca aaaatcacca gtctctctct acaaatctat ctctctctat
     9181 ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt
     9241 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     9301 tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacctgcag gcatgcgacg
     9361 tcgggccctc tagaggatcc ccgggtaccg cgaattatcg atcatgagcg gagaattaag
     9421 ggagtcacgt tatgaccccc gccgatgacg cgggacaagc cgttttacgt ttggaactga
     9481 cagaaccgca acgttgaagg agccactgag ccgcgggttt ctggagttta atgagctaag
     9541 cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag
     9601 caaatatttc ttgtcaaaaa tgctccactg acgttccata aattcccctc ggtatccaat
     9661 tagagtctca tattcactct caactcgaat cccccctatg aaaaagcctg aactcaccgc
     9721 gacgtctccc atatggtcga cctgcaggcg gccgcgaatt cgatggtggc ggccctagaa
     9781 tataacttcg tataatgtat gctatacgaa gttattagcg gccgggggat cgtacccggg
     9841 gatctggatt ttagtactgg attttggttt taggaattag aaattttatt gatagaagta
     9901 ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc
     9961 tataagaacc ctaatttccc ttatcgggaa actactcaca cattaggatc tagaactagt
    10021 ggatccccgt caccggtgtg agggaactag ttttgatctt gaaagatctt ttatctttag
    10081 agttaagaac tctttcgtat tttggtgagg ttttatcctc ttgagttttg gtcatagacc
    10141 tattcatggc tctgatacca atttttaagc gggggcttat gcggattatt tcttaaattg
    10201 ataaggggtt attagggggt atagggtata aatacaagca ttcccttagc gtatagtata
    10261 agtatagtag cgtacctcta tcaaatttcc atcttcttac cttgcacagg gcctgcaacc
    10321 ttatccttcc ttgtcttcct ccttccttcc gtccacttca tcatatttaa accaaaccta
    10381 cgggggagtc aacgtaacca accctgcctt agcatctttt ccctaacggc ctcctgccta
    10441 agcggtactt ctagcttcga acggcgtctg ggctccaggt ttagtcgtct cgtgtctggt
    10501 ttatattcac gacaaagatc tatagggact ttaggagatc tggattttag tactggattt
    10561 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
    10621 agggtttctt atatgctcaa cacatgagcg aaaccctata agaaccctaa tttcccttat
    10681 cgggaaacta ctcacacatt atttatggag aaaatagaga gagatagatt tgtagagaga
    10741 gactggtgat ttcagcgtac cgtccgaacc ccagagtccc gctcagtcct gctcctcggc
    10801 cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg
    10861 ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc
    10921 cgaccactcg gcgtacagct cgtccaggcc gcgcaccaac acccaggcca gggtgttgtc
    10981 cggcaccacc tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc
    11041 ggcgaagtcg tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac
    11101 cgctccggcg acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatgga
    11161 tcccctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg
    11221 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga
    11281 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
    11341 accactgtcg gcagaggcat cttcaacgat ggcctttcct ttatcgcaat gatggcattt
    11401 gtaggagcca ccttcctttt ccactatctt cacaataaag tgacagatag ctgggcaatg
    11461 gaatccgagg aggtttccgg atattaccct ttgttgaaaa gtctcaattg ccctttggtc
    11521 ttctgagact gtatctttga tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt
    11581 gacgaagatt ttcttcttgt cattgagtcg taagagactc tgtatgaact gttcgccagt
    11641 ctttacggcg agttctgtta ggtcctctat ttgaatcttt gactccatgg cctttgattc
    11701 agtgggaact acctttttag agactccaat ctctattact tgccttggtt tgtgaagcaa
    11761 gccttgaatc gtccatactg gaatagtact tctgatcttg agaaatatat ctttctctgt
    11821 gttcttgatg cagttagtcc tgaatctttt gactgcatct ttaaccttct tgggaaggta
    11881 tttgatttcc tggagattat tgctcgggta gatcgtcttg atgagacctg ctgcgtaagc
    11941 ctctctaacc atctgtgggt tagcattctt tctgaaaggg aattctagaa tataacttcg
    12001 tataatgtat gctatacgaa gttattagcg gccgggctgc aggaattccc gcggccgcca
    12061 tggcggccgg gagcatgcga cgtcgggccc aattcgccct atagtgagtc gtattacaat
    12121 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
    12181 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
    12241 cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat
    12301 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
    12361 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
    12421 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    12481 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    12541 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    12601 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
    12661 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
    12721 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
    12781 ccgcatcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    12841 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    12901 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    12961 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    13021 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    13081 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    13141 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    13201 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    13261 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    13321 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    13381 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    13441 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    13501 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    13561 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    13621 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    13681 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    13741 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    13801 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    13861 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    13921 accccgtaga aaagatcaaa ggatcttc
//
