LOCUS       sequence-219788-       10021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre/Lox C-terminal tagging construct encoding 3 copies of the 
            influenza virus hemagglutunin (HA) epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Watson AT1, Garcia V, Bone N, Carr AM, Armstrong J.
  TITLE     Gene tagging and gene replacement using recombinase-mediated 
            cassette exchange in Schizosaccharomyces pombe.
  JOURNAL   Gene. 2008 Jan 15;407(1-2):63-74
  PUBMED    18054176
REFERENCE   2  (bases 1 to 10021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2008-01-15"; volume: "407(1-2)"; pages: "63-7"
COMMENT     Sequence Label: pAW8_3HA
FEATURES             Location/Qualifiers
     source          1..10021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      575..1356
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(1451..1470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1570..1592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1630..1648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1821..1889,1890..2681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2039..2058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3341..3360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3594..3611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3728..3749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3764..3769,3770..3787,3788..3794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3807..3829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3826..3842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3826..3842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3875..3908)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3923..4012
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      4045..4232
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4100..4124)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            4307..5663
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4307..4650
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             4651..5460
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4718..4737)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      5466..5663
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(5757..5774)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5757..5773)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5766..5788)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8670..9764
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg
       61 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat
      121 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
      181 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc
      241 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag
      301 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc
      361 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt
      421 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa
      481 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg
      541 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat
      601 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt
      661 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag
      721 cgatcagcaa cttattctcc gttgtagatt ctttttcttc ataaattcca acaatgtaac
      781 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg
      841 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt
      901 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc
      961 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta
     1021 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt
     1081 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca
     1141 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat
     1201 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt
     1261 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact
     1321 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt
     1381 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt
     1441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3841 accatgatta cgccaagctt gcatgcgcgg ccgcataact tcgtatagca tacattatac
     3901 gaagttatgc atgctcatct tttacccata cgatgttcct gactatgcgg gctatcccta
     3961 tgacgtcccg gactatgcag gatcctatcc atatgacgtt ccagattacg ctgctcagtg
     4021 ctgaggcgcg ccacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa
     4081 taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga
     4141 aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagtat
     4201 gaggtcgctc ttattgacca cacctctacc ggcagatccg ctagggataa cagggtaata
     4261 tagatctgtt tagcttgcct cgtccccgcc gggtcacccg gccagcgaca tggaggccca
     4321 gaataccctc cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc
     4381 gtacatttag cccatacatc cccatgtata atcatttgca tccatacatt ttgatggccg
     4441 cacggcgcga agcaaaaatt acggctcctc gctgcagacc tgcgagcagg gaaacgctcc
     4501 cctcacagac gcgttgaatt gtccccacgc cgcgcccctg tagagaaata taaaaggtta
     4561 ggatttgcca ctgaggttct tctttcatat acttcctttt aaaatcttgc taggatacag
     4621 ttctcacatc acatccgaac ataaacaacc atgggtaagg aaaagactca cgtttcgagg
     4681 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     4741 gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg
     4801 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     4861 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     4921 gatgcatggt tactcaccac tgcgatcccc ggcaaaacag cattccaggt attagaagaa
     4981 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     5041 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     5101 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     5161 tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca
     5221 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     5281 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     5341 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     5401 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     5461 tcagtactga caataaaaag attcttgttt tcaagaactt gtcatttgta tagttttttt
     5521 atattgtagt tgttctattt taatcaaatg ttagcgtgat ttatattttt tttcgcctcg
     5581 acatcatctg cccagatgcg aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta
     5641 tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta acgccgccat ccagtcgacg
     5701 aactagtata acttcgtata tggtattata tacgaagtta tgcggccgct gaattcactg
     5761 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     5821 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     5881 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     5941 catctgtgcg gtatttcaca ccgcatacca tcgtccggat caatgaagta gatgagttta
     6001 ctacctgtat atactttttt ttttgtcaaa gtcactttta tggcatttcc atgttgaagg
     6061 attagccaca gcactaaatt ttcagcgtat gattgctttt aaatatttaa ttttcatcgt
     6121 tttttaatta atattcaaaa cgatttaatg ccttctacat ctgaagtcaa gctcatagac
     6181 tcgggttggg tagctaaaac tacatctaac acgaaggggt atagccttct taaagtatac
     6241 tcatgtataa acgttttggg ttcatatttt tgggccacgt ggtttataaa aattcttaac
     6301 tacaccactc ggtatcccgc acccgtctac gtttctacga tttcgagcta atattgagta
     6361 aagtgattag caaaaaaaat aaaagtactc gttgtcggag atcaagaatt tttctattat
     6421 ctcatctaaa ccactttcta aaagcgaaaa acaaaatcgt aatatgcagc ttgaatgggc
     6481 ttccatagtt tgaaagaaaa accctagcag tactggcaag ggagacattc cttttacctg
     6541 gctaatcgcc atcttccagc aggcgcacca ttgcccctgt ttcactatcc aggttacgga
     6601 tatagttcat gacaatattt acattggtcc agccaccagc ttgcatgatc tccggtattg
     6661 aaactccagc gcgggccata tctcgcgcgg ctccgacacg ggcactgtgt ccagaccagg
     6721 ccaggtatct ctgaccagag tcatccttag cgccgtaaat caatcgatga gttgcttcaa
     6781 aaatcccttc cagggcgcga gttgatagct ggctggtggc agatggcgcg gcaacaccat
     6841 tttttctgac ccggcaaaac aggtagttat tcggatcatc agctacacca gagacggaaa
     6901 tccatcgctc gaccagttta gttaccccca ggctaagtgc cttctctaca cctgcggtgc
     6961 taaccagcgt tttcgttctg ccaatatgga ttaacattct cccaccgtca gtacgtgaga
     7021 tatctttaac cctgatcctg gcaatttcgg ctatacgtaa cagggtgtta taagcaatcc
     7081 ccagaaatgc cagattacgt atatcctggc agcgatcgct attttccatg agtgaacgaa
     7141 cctggtcgaa atcagtgcgt tcgaacgcta gagcctgttt tgcacgttca ccggcatcaa
     7201 cgttttcttt tcggatccgc cgcataacca gtgaaacagc attgctgtca cttggtcgtg
     7261 gcagcccgga ccgacgatga agcatgttta gctggcccaa atgttgctgg atagttttta
     7321 ctgccagacc gcgcgcctga agatatagaa gataatcgcg aacatcttca ggttctgcgg
     7381 gaaaccattt ccggttattc aacttgcacc atgccgccca cgaccggcaa acggacagaa
     7441 gcattttcca ggtatgctca gaaaacgcct ggcgatccct gaacatgtcc atcaggttct
     7501 tgcgaacctc atcactcgtt gcatcgaccg gtaactttta gattaacatt tgtttaaata
     7561 tgaaattagg caatgtaaca tactgcaggc aaattttggt gtacggtaag taaattggac
     7621 atatgattta acaaagcgac tataagtcag aaagtgagaa tgagattgaa ataattaatt
     7681 caacttattc aattgatgat atgccaggat tcctcttcct ttatcttcaa tgtttcgatt
     7741 aacaggtcca ttatccggtt tttagtttcg ctgcacattg ccgaatgaca atcggattcc
     7801 tttaagcttt gcttttgccc tgttttctct ccccagtaat cgttgggtga ctgaaccatc
     7861 cagtagccga gagttacgtt tttcctgatt taataaatat ccatatctat ttgtctcgtg
     7921 gtgttttaca gcaactaacc gaaattacta ttttgcatca tccaaccata aagagacaac
     7981 aataattcta gaaaacatat aacaagtgtt tttttctatt tacgtctcat acgcaaagtc
     8041 cactttacca tgttttggaa acttgtaccc aaccattcat ttttcgattt tgtaatgagg
     8101 ctcttttctt ctaacaatca ttgaggtcat tatcatgtgt cctcacctat tgtttaaatt
     8161 gaggcttgcg ctattcccaa aattgtgggt attattttca tatatcagta tctgcaattt
     8221 cggttcaact ccggaagctc ggtacccaac tgtgggaata ctcaggtatc gtaagatgca
     8281 agagttcgaa tctcttagca accattattt tttttctctc aacataacga gaacacacag
     8341 gggcgctatc gcacagaatc aaattcgatg actggaaatt ttttgttaat ttcagaggtc
     8401 gcctgacgca tatacctttt tcaactgaaa aattgggaga aaaaggaaag gtgagagcgc
     8461 cggaaccggc ttttcatata gaatagagaa gcgttcatga ctaaatgctt gcatcacaat
     8521 acttgaagtt gacaatatta tttaaggacc tattgttttt tccaataggt ggttagcaat
     8581 cgtcttactt tctaactttt cttacctttt acatttcagc aatatatata tatatatata
     8641 tatatatatt tcaaggatat accattgtaa tgtctgcccc taagaagatc gtcgttttgc
     8701 caggtgacca cgttggtcaa gaaatcacag ccgaagccat taaggttctt aaagctattt
     8761 ctgatgttcg ttccaatgtc aagttcgatt tcgaaaatca tttaattggt ggtgctgcta
     8821 tcgatgctac aggtgttcca cttccagatg aggcgctgga agcctccaag aaggctgatg
     8881 ccgttttgtt aggtgctgtg ggtggtccta aatggggtac cggtagtgtt agacctgaac
     8941 aaggtttact aaaaatccgt aaagaacttc aattgtacgc caacttaaga ccatgtaact
     9001 ttgcatccga ctctctttta gacttatctc caatcaagcc acaatttgct aaaggtactg
     9061 acttcgttgt tgttagagaa ttagtgggag gtatttactt tggtaagaga aaggaagacg
     9121 atggtgatgg tgtcgcttgg gatagtgaac aatacaccgt tccagaagtg caaagaatca
     9181 caagaatggc cgctttcatg gccctacaac atgagccacc attgcctatt tggtccttgg
     9241 ataaagctaa tgttttggcc tcttcaagat tatggagaaa aactgtggag gaaaccatca
     9301 agaacgaatt tcctacattg aaagttcaac atcaattgat tgattctgcc gccatgatcc
     9361 tagttaagaa cccaacccac ctaaatggta ttataatcac cagcaacatg tttggtgata
     9421 tcatctccga tgaagcctcc gttatcccag gctccttggg tttgttgcca tctgcgtcct
     9481 tggcctcttt gccagacaag aacaccgcat ttggtttgta cgaaccatgc catggttccg
     9541 ctccagattt gccaaagaat aaggtcaacc ctatcgccac tatcttgtct gctgcaatga
     9601 tgttgaaatt gtcattgaac ttgcctgaag aaggtaaagc cattgaagat gcagttaaaa
     9661 aggttttgga tgcaggtatc agaactggtg atttaggtgg ttccaacagt accaccgaag
     9721 tcggtgatgc tgtcgccgaa gaagttaaga aaatccttgc ttaaaaagat tctctttttt
     9781 tgtgatattt gtacataaac tttataaatg aaattcataa tagaaacgac acgaaattac
     9841 aaaatggaat atgttcatag ggtagacgaa actatatacg caatctacat acatttatca
     9901 agaaggagaa aaaggaggat gtaaaggaat acaggtaagc aaattgatac taatggctca
     9961 acgtgataag gaaaaagaat tgcactttaa cattaatatt gacaaggagg agggcatcac
    10021 a
//
