LOCUS       sequence-219792-        7550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast optimized mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Albakri MB, Jiang Y, Genereaux J, Lajoie P
  TITLE     Polyglutamine toxicity assays highlight the advantages of mScarlet 
            for imaging in Saccharomyces cerevisiae.
  JOURNAL   F1000Res. 2018 Aug 10;7:1242. doi: 10.12688/f1000research.15829.2. 
            eCollection 2018.
  PUBMED    30631438
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2018-08-10"; volume: "7:1242. doi"; pages: " 
            10.12688/f1000research.15829.2. eCollection 2018"
COMMENT     Sequence Label: ymScarlet
FEATURES             Location/Qualifiers
     source          1..7550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1055..1698
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     1592..1615
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     terminator      2433..2680
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2449..2467)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(2698..2717)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2699..2717)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2727..2744)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2727..2743)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2736..2758)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2884..3339
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2971..2990)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3181..3202
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3640..4047
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             4048..5142
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(5636..5655)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5755..5777
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5815..5833)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5875..6378
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6415..6519
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6520..6588,6589..7380)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6738..6757)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcag tttatcatta tcaatactcg ccatttcaaa
     1081 gaatacgtaa ataattaata gtagtgattt tcctaacttt atttagtcaa aaaattagcc
     1141 ttttaattct gctgtaaccc gtacatgccc aaaatagggg gcgggttaca cagaatatat
     1201 aacatcgtag gtgtctgggt gaacagttta ttcctggcat ccactaaata taatggagcc
     1261 cgctttttaa gctggcatcc agaaaaaaaa agaatcccag caccaaaata ttgttttctt
     1321 caccaaccat cagttcatag gtccattctc ttagcgcaac tacagagaac aggggcacaa
     1381 acaggcaaaa aacgggcaca acctcaatgg agtgatgcaa cctgcctgga gtaaatgatg
     1441 acacaaggca attgacccac gcatgtatct atctcatttt cttacacctt ctattacctt
     1501 ctgctctctc tgatttggaa aaagctgaaa aaaaaggttg aaaccagttc cctgaaatta
     1561 ttcccctact tgactaataa gtatataaag acggtaggta ttgattgtaa ttctgtaaat
     1621 ctatttctta aacttcttaa attctacttt tatagttagt ctttttttta gttttaaaac
     1681 accagaactt agtttcgacg gattctagaa ctagtctaaa aatggtttct aaaggtgaag
     1741 cagttattaa ggaattcatg agattcaagg tacacatgga aggttctatg aatggtcacg
     1801 aatttgaaat tgaaggtgaa ggtgaaggta gaccatatga aggtactcaa actgctaagt
     1861 tgaaggttac taaaggtggt ccattgccat tttcttggga tattttgtct ccacaattca
     1921 tgtacggttc tagagctttt acaaaacatc cagcagatat tccagattac tacaagcaat
     1981 cattcccaga aggttttaaa tgggaaagag ttatgaactt cgaagatggt ggtgcagtta
     2041 ctgttacaca agatacttct ttggaagatg gtacattgat ctataaggtt aagttgagag
     2101 gtactaattt tccaccagat ggtccagtta tgcaaaagaa aactatgggt tgggaagctt
     2161 caacagaaag attgtaccca gaagatggtg ttttgaaggg tgacattaag atggcattga
     2221 gattgaagga tggtggtaga tatttggctg atttcaagac tacatacaag gctaagaaac
     2281 cagttcaaat gccaggtgct tacaacgttg atagaaagtt ggatattact tctcataatg
     2341 aagattacac agttgttgaa caatatgaaa gaagtgaagg tagacacagt acaggtggta
     2401 tggatgaatt atacaaatga gtcgacctcg agtcatgtaa ttagttatgt cacgcttaca
     2461 ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt
     2521 ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat ttatatttca
     2581 aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt
     2641 gcttgagaag gttttgggac gctcgaaggc tttaatttgc ggccggtacc caattcgccc
     2701 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     2761 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     2821 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     2881 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     2941 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3001 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3061 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3121 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3181 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     3241 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3301 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct
     3361 tacgcatctg tgcggtattt cacaccgcat atcgaccggt cgaggagaac ttctagtata
     3421 tctacatacc taatattatt gccttattaa aaatggaatc ccaacaatta catcaaaatc
     3481 cacattctct tcaaaatcaa ttgtcctgta cttccttgtt catgtgtgtt caaaaacgtt
     3541 atatttatag gataattata ctctatttct caacaagtaa ttggttgttt ggccgagcgg
     3601 tctaaggcgc ctgattcaag aaatatcttg accgcagtta actgtgggaa tactcaggta
     3661 tcgtaagatg caagagttcg aatctcttag caaccattat ttttttcctc aacataacga
     3721 gaacacacag gggcgctatc gcacagaatc aaattcgatg actggaaatt ttttgttaat
     3781 ttcagaggtc gcctgacgca tatacctttt tcaactgaaa aattgggaga aaaaggaaag
     3841 gtgagagcgc cggaaccggc ttttcatata gaatagagaa gcgttcatga ctaaatgctt
     3901 gcatcacaat acttgaagtt gacaatatta tttaaggacc tattgttttt tccaataggt
     3961 ggttagcaat cgtcttactt tctaactttt cttacctttt acatttcagc aatatatata
     4021 tatatatttc aaggatatac cattctaatg tctgccccta agaagatcgt cgttttgcca
     4081 ggtgaccacg ttggtcaaga aatcacagcc gaagccatta aggttcttaa agctatttct
     4141 gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt taattggtgg tgctgctatc
     4201 gatgctacag gtgttccact tccagatgag gcgctggaag cctccaagaa ggctgatgcc
     4261 gttttgttag gtgctgtggg tggtcctaaa tggggtaccg gtagtgttag acctgaacaa
     4321 ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca acttaagacc atgtaacttt
     4381 gcatccgact ctcttttaga cttatctcca atcaagccac aatttgctaa aggtactgac
     4441 ttcgttgttg tcagagaatt agtgggaggt atttactttg gtaagagaaa ggaagacgat
     4501 ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc cagaagtgca aagaatcaca
     4561 agaatggccg ctttcatggc cctacaacat gagccaccat tgcctatttg gtccttggat
     4621 aaagctaatg ttttggcctc ttcaagatta tggagaaaaa ctgtggagga aaccatcaag
     4681 aacgaattcc ctacattgaa ggttcaacat caattgattg attctgccgc catgatccta
     4741 gttaagaacc caacccacct aaatggtatt ataatcacca gcaacatgtt tggtgatatc
     4801 atctccgatg aagcctccgt tatcccaggt tccttgggtt tgttgccatc tgcgtccttg
     4861 gcctctttgc cagacaagaa caccgcattt ggtttgtacg aaccatgcca cggttctgct
     4921 ccagatttgc caaagaataa ggtcaaccct atcgccacta tcttgtctgc tgcaatgatg
     4981 ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca ttgaagatgc agttaaaaag
     5041 gttttggatg caggtatcag aactggtgat ttaggtggtt ccaacagtac caccgaagtc
     5101 ggtgatgctg tcgccgaaga agttaagaaa atccttgctt aaaaagattc tcttttttta
     5161 tgatatttgt acataaactt tataaatgaa attcataata gaaacgacac gaaattacaa
     5221 aatggaatat gttcataggg tagacgaaac tatatacgca atctacatac atttatcaag
     5281 aaggagaaaa aggaggatgt aaaggaatac aggtaagcaa attgatacta atggctcaac
     5341 gtgataagga aaaagaattg cactttaaca ttaatattga caaggaggag ggcaccacac
     5401 aaaaagttag gtgtaacaga aaatcatgaa actatgattc ctaatttata tattggagga
     5461 ttttctctaa aaaaaaaaaa atacaacaaa taaaaaacac tcaatgacct gaccatttga
     5521 tggagtttaa gtcaatacct tcttgaacca tttcccataa tggtgaaagt tccctcaaga
     5581 attttactct gtcagaaacg gccttaacga cgtagtcgat atggtgcact ctcagtacaa
     5641 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5701 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5761 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5821 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag gacggatcgc
     5881 ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg gaataatttg ggaatttact
     5941 ctgtgtttat ttatttttat gttttgtatt tggattttag aaagtaaata aagaaggtag
     6001 aagagttacg gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc aacaaaaagc
     6061 gtactttaca tatatattta ttagacaaga aaagcagatt aaatagatat acattcgatt
     6121 aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac acagacaaga
     6181 tgaaacaatt cggcattaat acctgagagc aggaagagca agataaaagg tagtatttgt
     6241 tggcgatccc cctagagtct tttacatctt cggaaaacaa aaactatttt ttctttaatt
     6301 tcttttttta ctttctattt ttaatttata tatttatatt aaaaaattta aattataatt
     6361 atttttatag cacgtgatga aaaggaccca ggtggcactt ttcggggaaa tgtgcgcgga
     6421 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6481 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6541 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6601 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6661 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6721 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     6781 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6841 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6901 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6961 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     7021 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     7081 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7141 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7201 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     7261 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7321 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     7381 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7441 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7501 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
