LOCUS       sequence-219793-        7549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast optimized FusionRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Albakri MB, Jiang Y, Genereaux J, Lajoie P
  TITLE     Polyglutamine toxicity assays highlight the advantages of mScarlet 
            for imaging in Saccharomyces cerevisiae.
  JOURNAL   F1000Res. 2018 Aug 10;7:1242. doi: 10.12688/f1000research.15829.2. 
            eCollection 2018.
  PUBMED    30631438
REFERENCE   2  (bases 1 to 7549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2018-08-10"; volume: "7:1242. doi"; pages: " 
            10.12688/f1000research.15829.2. eCollection 2018"
COMMENT     Sequence Label: yFusionRed
FEATURES             Location/Qualifiers
     source          1..7549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1055..1698
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(2419..2435)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2420..2436)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      2438..2685
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2454..2472)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(2703..2722)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2704..2722)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2732..2749)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2732..2748)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2741..2763)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2890..3344
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2976..2995)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3186..3207
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3645..4052
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     5754..5776
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5814..5832)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5874..6377
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6414..6518
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6519..6587,6588..7379)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6737..6756)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcag tttatcatta tcaatactcg ccatttcaaa
     1081 gaatacgtaa ataattaata gtagtgattt tcctaacttt atttagtcaa aaaattagcc
     1141 ttttaattct gctgtaaccc gtacatgccc aaaatagggg gcgggttaca cagaatatat
     1201 aacatcgtag gtgtctgggt gaacagttta ttcctggcat ccactaaata taatggagcc
     1261 cgctttttaa gctggcatcc agaaaaaaaa agaatcccag caccaaaata ttgttttctt
     1321 caccaaccat cagttcatag gtccattctc ttagcgcaac tacagagaac aggggcacaa
     1381 acaggcaaaa aacgggcaca acctcaatgg agtgatgcaa cctgcctgga gtaaatgatg
     1441 acacaaggca attgacccac gcatgtatct atctcatttt cttacacctt ctattacctt
     1501 ctgctctctc tgatttggaa aaagctgaaa aaaaaggttg aaaccagttc cctgaaatta
     1561 ttcccctact tgactaataa gtatataaag acggtaggta ttgatkgtaa ttctgtaaat
     1621 ctatttctta aacttcttaa attctacttt tatagttagt ctttttttta gttttaaaac
     1681 accagaactt agtttcgacg gattctagaa ctaaaaatgg tttctgaatt gattaaagaa
     1741 aacatgccaa tgaagttgta catggaaggt actgttaaca accatcattt taaatgtaca
     1801 tcagaaggtg aaggtaaacc atacgaaggt actcaaacaa tgagaattaa agttgttgaa
     1861 ggtggtccat tgccatttgc tttcgatatt ttggcaactt cttttatgta cggttcaaga
     1921 acttttatta agcatccacc aggtattcca gatttcttta agcaatcttt cccagaaggt
     1981 tttacttggg aaagagttac tacatatgaa gatggtggtg ttttgactgc aacacaagat
     2041 acatcattgc aagatggttg tttgatctat aacgttaaag ttagaggtgt taattttccm
     2101 gctaatggtc cagttatgca aaagaaaact ttgggttggg aagcttctac tgaaacaatg
     2161 tacccagcag atggtggttt agaaggtgct tgtgatatgg cattgaaatt ggttggtggt
     2221 ggtcatttga tctgtaattt ggaaactaca tacagatcta agaaaccagc tacaaatttg
     2281 aagatgccag gtgtttacaa cgttgatcat agattggaaa gaattaaaga agcagatgat
     2341 gaaacttacg ttgaacaaca tgaagttgct gttgcaagat actctacagg tggtgctggt
     2401 gacggtggta aataatatcg ataccgtcga cctcgagtca tgtaattagt tatgtcacgc
     2461 ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct
     2521 gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata
     2581 tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa
     2641 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcggccg gtacccaatt
     2701 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     2761 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     2821 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     2881 gcgaatgggc gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     2941 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3001 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3061 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     3121 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3181 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     3241 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     3301 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt
     3361 ctccttacgc atctgtgcgg tatttcacac cgcatatcga ccggtcgagg agaacttcta
     3421 gtatatctac atacctaata ttattgcctt attaaaaatg gaatcccaac aattacatca
     3481 aaatccacat tctcttcaaa atcaattgtc ctgtacttcc ttgttcatgt gtgttcaaaa
     3541 acgttatatt tataggataa ttatactcta tttctcaaca agtaattggt tgtttggccg
     3601 agcggtctaa ggcgcctgat tcaagaaata tcttgaccgc agttaactgt gggaatactc
     3661 aggtatcgta agatgcaaga gttcgaatct cttagcaacc attatttttt tcctcaacat
     3721 aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg aaattttttg
     3781 ttaatttcag aggtcgcctg acgcatatac ctttttcaac tgaaaaattg ggagaaaaag
     3841 gaaaggtgag agcgccggaa ccggcttttc atatagaata gagaagcgtt catgacctga
     3901 tgcttgcatc acaatacttt aagttgacaa tattatttaa ggacctattg ttttttccaa
     3961 taggtggtta gcaatcgtct tactttctaa cttttcttac cttttacatt tcagcaatat
     4021 atatatatat atttcaagga tataccattc taatgtctgc ccctaagaag atcgtcgttt
     4081 tgccaggtga ccacgttggt caagaaatca cagccgaagc cattaaggtt cttaaagcta
     4141 tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg
     4201 ctatcgatgc tacaggtgtt ccacttccag atgaggcgct ggaagcctcc aagaaggctg
     4261 atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg taccggtagt gttagacctg
     4321 aacaaggttt actaaaaatc cgtaaagaac ttcaattgta cgccaactta agaccatgta
     4381 actttgcatc cgactctctt ttagacttat ctccaatcaa gccacaattt gctaaaggta
     4441 ctgacttcgt tgttgtcaga gaattagtgg gaggtattta ctttggtaag agaaaggaag
     4501 acgatggtga tggtgtcgct tgggatagtg aacaatacac cgttccagaa gtgcaaagaa
     4561 tcacaagaat ggccgctttc atggccctac aacatgagcc accattgcct atttggtcct
     4621 tggataaagc taatgttttg gcctcttcaa gattatggag aaaaactgtg gaggaaacca
     4681 tcaagaacga attccctaca ttgaaggttc aacatcaatt gattgattct gccgccatga
     4741 tcctagttaa gaacccaacc cacctaaatg gtattataat caccagcaac atgtttggtg
     4801 atatcatctc cgatgaagcc tccgttatcc caggttcctt gggtttgttg ccatctgcgt
     4861 ccttggcctc tttgccagac aagaacaccg catttggttc ccatgccacg gttctgctcc
     4921 agatttgcca aagaataagg tcaaccctat cgccactatc ttgtctgctg caatgatgtt
     4981 gaaattgtca ttgaacttgc ctgaagaagg taaggccatt gaagatgcag ttaaaaaggt
     5041 tttggatgca ggtatcagaa ctggtgattt aggtggttcc aacagtacca ccgaagtcgg
     5101 tgatgctgtc gccgaagaag ttaagaaaat ccttgcttaa aaagattctc tttttttatg
     5161 atatttgtac ataaacttta taaatgaaat tcataataga aacgacacga aattacaaaa
     5221 tggaatatgt tcatagggta gacgaaacta tatacgcaat ctacatacat ttatcaagaa
     5281 ggagaaaaag gaggatgtaa aggaatacag gtaagcaaat tgatactaat ggctcaacgt
     5341 gataaggaaa aagaattgca ctttaacatt aatattgaca aggaggaggg caccacacaa
     5401 aaagttaggt gtaacagaaa atcatgaaac tatgattcct aatttatata ttggaggatt
     5461 ttctctaaaa aaaaaaaaan tacaacaaat aaaaaacact caatgacctg accatttgat
     5521 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa
     5581 ttttactctg tcagaaacgg ccttaacgac gtagtcgata tggtgcactc tcagtacaat
     5641 ctgctctgat gcckcatagt taagccmscc ccgacacccg ccaacacccg ctgacgcgcc
     5701 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     5761 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     5821 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttagg acggatcgct
     5881 tgcctgtaac ttacacgcgc ctcgtatctt ttaatgatgg aataatttgg gaatttactc
     5941 tgtgtttatt tatttttatg ttttgtattt ggattttaga aagtaaataa agaaggtaga
     6001 agagttacgg aatgaagaaa aaaaaataaa caaaggttta aaaaatttca acaaaaagcg
     6061 tactttacat atatatttat tagacaagaa aagcagatta aatagatata cattcgatta
     6121 acgataagta aaatgtaaaa tcacaggatt ttcgtgtgtg gtcttctaca cagacaagat
     6181 gaaacaattc ggcattaata cctgagagca ggaagagcaa gataaaaggt agtatttgtt
     6241 ggcgatcccc ctagagtctt ttacatcttc ggaaaacaaa aactattttt tctttaattt
     6301 ctttttttac tttctatttt taatttatat atttatatta aaaaatttaa attataatta
     6361 tttttatagc acgtgatgaa aaggacccag gtggcacttt tcggggaaat gtgcgcggaa
     6421 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6481 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6541 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6601 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6661 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6721 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6781 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6841 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6901 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6961 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7021 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7081 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7141 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7201 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7261 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7321 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7381 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7441 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7501 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
