LOCUS       sequence-219832-        1116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear localized GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Economo MN, Viswanathan S, Tasic B, Bas E, Winnubst J, Menon V, 
            Graybuck LT, Nguyen TN, Smith KA, Yao Z, Wang L, Gerfen CR, 
            Chandrashekar J, Zeng H, Looger LL, Svoboda K
  TITLE     Distinct descending motor cortex pathways and their roles in 
            movement.
  JOURNAL   Nature. 2018 Nov;563(7729):79-84. doi: 10.1038/s41586-018-0642-9. 
            Epub 2018 Oct 31.
  PUBMED    30382200
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0642-9"; journalName: "Nature"; date: "2018-11";
            volume: "563"; issue: "7729"; pages: "79-84"
COMMENT     Sequence Label: pAAV-CAG-H2B-GFP
FEATURES             Location/Qualifiers
     source          1..1116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..378
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKENYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      391..400
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(397..399,400..402,403..1116)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(442..463)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(703..722)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1050..1071
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgccagagc cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact
       61 aaggcgcaga agaaaggcgg caagaagcgc aagcgcagcc gcaaggagaa ctattccatc
      121 tatgtgtaca aggttctgaa gcaggtccac cctgacaccg gcatttcgtc caaggccatg
      181 ggcatcatga attcgtttgt gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc
      241 ctggcgcatt acaacaagcg ctcgaccatc acctccaggg agatccagac ggccgtgcgc
      301 ctgctgctgc ctggggagtt ggccaagcac gccgtgtccg agggtactaa ggccatcacc
      361 aagtacacca gcgctaagga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
      421 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      481 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      541 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
      601 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      661 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      721 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      781 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
      841 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
      901 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
      961 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
     1021 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1081 gggatcactc tcggcatgga cgagctgtac aagtaa
//
