LOCUS       sequence-219836-        1830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear localized tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Economo MN, Viswanathan S, Tasic B, Bas E, Winnubst J, Menon V, 
            Graybuck LT, Nguyen TN, Smith KA, Yao Z, Wang L, Gerfen CR, 
            Chandrashekar J, Zeng H, Looger LL, Svoboda K
  TITLE     Distinct descending motor cortex pathways and their roles in 
            movement.
  JOURNAL   Nature. 2018 Nov;563(7729):79-84. doi: 10.1038/s41586-018-0642-9. 
            Epub 2018 Oct 31.
  PUBMED    30382200
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0642-9"; journalName: "Nature"; date: "2018-11";
            volume: "563"; issue: "7729"; pages: "79-84"
COMMENT     Sequence Label: pAAV-CAG-H2B tdTomato
FEATURES             Location/Qualifiers
     source          1..1830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..378
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      391..400
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             397..1827
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKPPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 atgccagagc cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact
       61 aaggcgcaga agaaaggcgg caagaagcgc aagcgcagcc gcaaggagag ctattccatc
      121 tatgtgtaca aggttctgaa gcaggtccac cctgacaccg gcatttcgtc caaggccatg
      181 ggcatcatga attcgtttgt gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc
      241 ctggcgcatt acaacaagcg ctcgaccatc acctccaggg agatccagac ggccgtgcgc
      301 ctgctgctgc ctggggagtt ggccaagcac gccgtgtccg agggtactaa ggccatcacc
      361 aagtacacca gcgctaagga tccaccggtc gccaccatgg tgagcaaggg cgaggaggtc
      421 atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc
      481 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      541 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac
      601 ggctccaagg cgtacgtgaa gccccccgcc gacatccccg attacaagaa gctgtccttc
      661 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg
      721 acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc
      781 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
      841 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg
      901 aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg
      961 caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac
     1021 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt cctggggcat
     1081 ggcaccggca gcaccggcag cggcagctcc ggcaccgcct cctccgagga caacaacatg
     1141 gccgtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac
     1201 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     1261 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
     1321 atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg
     1381 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg
     1441 accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc
     1501 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     1561 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg
     1621 aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag
     1681 cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac
     1741 gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg
     1801 tacggcatgg acgagctgta caagtaataa
//
