LOCUS       sequence-219837-        1347 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear localized mRuby2FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Economo MN, Viswanathan S, Tasic B, Bas E, Winnubst J, Menon V, 
            Graybuck LT, Nguyen TN, Smith KA, Yao Z, Wang L, Gerfen CR, 
            Chandrashekar J, Zeng H, Looger LL, Svoboda K
  TITLE     Distinct descending motor cortex pathways and their roles in 
            movement.
  JOURNAL   Nature. 2018 Nov;563(7729):79-84. doi: 10.1038/s41586-018-0642-9. 
            Epub 2018 Oct 31.
  PUBMED    30382200
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0642-9"; journalName: "Nature"; date: "2018-11";
            volume: "563"; issue: "7729"; pages: "79-84"
COMMENT     Sequence Label: pAAV- CAG-h2BmRuby2FLAG
FEATURES             Location/Qualifiers
     source          1..1347
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..378
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             394..417
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             421..444
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             448..471
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             976..999
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1006..1029
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1042..1065
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1072..1095
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1288..1311
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1315..1338
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgccagagc cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact
       61 aaggcgcaga agaaaggcgg caagaagcgc aagcgcagcc gcaaggagag ctattccatc
      121 tatgtgtaca aggttctgaa gcaggtccac cctgacaccg gcatttcgtc caaggccatg
      181 ggcatcatga attcgtttgt gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc
      241 ctggcgcatt acaacaagcg ctcgaccatc acctccaggg agatccagac ggccgtgcgc
      301 ctgctgctgc ctggggagtt ggccaagcac gccgtgtccg agggtactaa ggccatcacc
      361 aagtacacca gcgctaagga tccaggtacc atggactaca aggacgacga cgacaaaggt
      421 gactacaaag atgatgacga taaaggcgac tataaggacg atgacgacaa gggcggaaac
      481 tcactgatca aggaaaacat gcggatgaag gtggtgatgg agggctccgt gaatggtcac
      541 cagttcaagt gcaccggaga gggagaggga aacccgtaca tgggaactca gaccatgcgc
      601 attaaggtca tcgaaggagg tccgctgccg ttcgctttcg atatcctggc cacttcgttc
      661 ggaggagggt cgcgcacgtt catcaagtac ccgaagggaa tcccggactt ctttaagcag
      721 tcattcccgg aaggattcac ttgggaacgg gtgacccggt atgaagatgg aggtgtggtg
      781 actgtcatgc aagatacttc gctggaggat gggtgcctcg tgtaccacgt ccaagtccgc
      841 ggagtgaatt tcccgtccaa cggaccagtg atgcagaaaa agacgaaggg ttgggaacct
      901 aatactgaaa tgatgtaccc cgcagacgga gggctgaggg gctacaccca catggcgctg
      961 aaggtcgacg gaggagatta caaggatgac gacgataagc aacaagatta caaagacgat
     1021 gatgacaagg gccagcaggg cgactacaag gacgacgacg acaagcagca ggactacaaa
     1081 gatgacgatg ataaaggagg aggacatctg tcctgttcgt tcgtgaccac ctacagatca
     1141 aagaaaaccg tgggaaacat caagatgccg ggcattcatg ccgtcgacca ccgcctggag
     1201 cggctcgaag aatcagacaa tgagatgttc gtcgtgcaaa gagaacatgc cgtggccaag
     1261 ttcgcgggac tgggaggcgg tggaggcgat tacaaagacg atgatgacaa gggtgactat
     1321 aaagacgacg atgacaaagg ggattaa
//
