LOCUS       sequence-219843-        9361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 9361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pBPZpGAL4DBDUw
FEATURES             Location/Qualifiers
     source          1..9361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     44..62
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    90..214
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(239..244,245..262,263..269)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             323..982
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(389..408)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1324..1629
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1508..1527
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1670..1794)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2162..2602
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     complement(2863..2879)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2863..2879)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(3890..4504,4575..4706,4910..5225,5287..5941,
                     6016..6289,6691..6762))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     7214..7232
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    7457..7526
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(7613..8201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7693..7712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8372..9163,9164..9232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8995..9014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9233..9337)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
       61 gccctttcgt cttcaagaat tcgtttatca caagtttgta caaaaaagct gaacgagaaa
      121 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
      181 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
      241 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
      301 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      361 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      421 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      481 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      541 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      601 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      661 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      721 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      781 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      841 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      901 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      961 tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta
     1021 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     1081 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     1141 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     1201 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     1261 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     1321 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     1381 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     1441 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     1501 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     1561 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     1621 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     1681 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     1741 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgataaa
     1801 cggccggccg agctcgcccg gggatcgagc gcagcggtat aaaagggcgc ggggtggctg
     1861 agagcatcag ttgtgaatga atgttcgagc cgagcagacg tgccgctgcc ttcgttaata
     1921 tcctttgaat aagccaactt tgaatcacaa gacgcatacc aaacggtacc aaaacatgct
     1981 ggagatccgc gccgccttcc tgcgccagcg caacaccgcc ctgcgcaccg aggtggccga
     2041 gctggagcag gaggtgcagc gcctggagaa cgaggtgagc cagtacgaga cccgctacgg
     2101 ccccctgggc ggcggcaagg ctagcggagg aggtggtgga ggtggaggtg gaggtactag
     2161 tatgaagctg ctgagtagta ttgaacaagc gtgtgacata tgccgactta agaagctgaa
     2221 gtgcagcaag gaaaagccga agtgcgccaa atgtcttaag aacaattggg agtgccggta
     2281 ctcgccaaag acgaagcgaa gccctttgac acgcgctcat ttgactgaag tggagagtcg
     2341 cctggagcgc cttgagcagt tgttcctcct catttttccc cgtgaagacc tcgacatgat
     2401 actgaagatg gatagtctcc aagatattaa ggccctgctc actggcctgt ttgttcagga
     2461 taacgtcaac aaggatgccg tgacggaccg ccttgcctcg gtggagacag atatgccact
     2521 taccttgcgg cagcatcgca tctcggctac cagcagtagt gaagaaagtt ccaataaggg
     2581 tcaacggcaa ctgacggtat cgtaaaagct tatcgatacc gtcgactaaa gccaaataga
     2641 aaattattca gttcctggct taagttttta aaagtgatat tatttatttg gttgtaacca
     2701 accaaaagaa tgtaaataac taatacataa ttatgttagt tttaagttag caacaaattg
     2761 attttagcta tattagctac ttggttaata aatagaatat atttatttaa agataattgc
     2821 gtttttattg tcagggagtg agtttgctta aaaactcgtt tagatccact agttctagag
     2881 cggccgctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg
     2941 gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga
     3001 ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt
     3061 ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggaggcgcgc
     3121 cctagttcca gtgaaatcca agcattttct aaattaaatg tattcttatt attatagttg
     3181 ttatttttga tatatataaa caacactatt atgcccacca tttttttgag atgcatctac
     3241 acaaggaaca aacactggat gtcactttca gttcaaattg taacgctaat cactccgaac
     3301 aggtcacaaa aaattacctt aaaaagtcat aatattaaat tagaataaat atagctgtga
     3361 gggaaatata tacaaatata ttggagcaaa taaattgtac atacaaatat ttattactaa
     3421 tttctattga gacgaaatga accactcgga accatttgag cgaaccgaat cgcgcggaac
     3481 taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg
     3541 gagacagcga aagagcaact acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg
     3601 cgcgatttga aaagtatgta tataaaaaat atatcccggt gttttatgta gcgataaacg
     3661 agtttttgat gtaaggtatg caggtgtgta agtcttttgg ttagaagaca aatccaaagt
     3721 ctacttgtgg ggatgttcga aggggaaata cttgtattct ataggtcata tcttgttttt
     3781 attggcacaa atataattac attagctttt tgagggggca ataaacagta aacacgatgg
     3841 taataatggt aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc tactccttgc
     3901 gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg atgagaatgg
     3961 ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc tccaggatga
     4021 ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg cccggctcca
     4081 cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat gagaggtacg
     4141 acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaagccg ccaaagagca
     4201 ggaatggtat gataaccggc ggacccacag acagcgccat cgaggtcgag gagctggcgc
     4261 aggatattag atatccgaag gacgttgaca cattggccac cagagtgacc agcgccaggc
     4321 agttgaagaa gtgcagcact ccggcccgca gtccgatcat cggataggca atcgccgtga
     4381 agaccagtgg cactgtgaga aaaagcggca attcggcaat cgttttgccc agaaagtatg
     4441 tgtcacagcg ataaagtcga cttcgggcct ccctcataaa aactggcagc tctgaggtga
     4501 acacctaaat cgaatcgatt cattagaaag ttagtaaatt attgaaatgc aaatgtattc
     4561 taaacatgac ttacatttat cgtggcaaag acgttttgaa aggtcatgtt ggtcaggaag
     4621 aggaagatgg ctccgttgat attcatcaca cccacttgcg tgagttgttg gcccaaaaag
     4681 atgaggccaa tcaagatggc aaccatctgc aaattaaaat gttactcgca tctcattaat
     4741 attcgcgagt taaatgaaat ttatttatct tctgcaaaac tataaactat acatctcatt
     4801 gaaaaaaact aagaagggtg tggaatcagg caattctatc taaaatctag cgaatttgtt
     4861 tccaagaatt gtaagcgtta tatcatttgt ttccactgga accactcacc gttgtctgaa
     4921 taagtcgcac ttttacgagg agtggttcct tgagcaccga cagccaggat cgccacagga
     4981 ccgcccggaa ctgcatgaac caggtggcct tgtaggtgta cccattctcc ggctgctcca
     5041 gtggcttctc cagatttttg gtggccaaca actgctccat atcccgggct actttgctaa
     5101 tggcaaaatt gtcgcatatc ttggcgatcc gatcacggga ctcgatctcc cgtccgggca
     5161 caacggccaa cacctgtacg taaaagtccg ccggattgta gttggtagga cactgggcac
     5221 ccacgctgga taggagttga gatgttatgt aatactagat acccttaata aacacatcga
     5281 actcactagg aaaagaagtc gacggcttcg ctgggagtgc ccaagaaagc taccctgccc
     5341 tcggccatca gaaggatctt gtcaaagagc tcaaacagct cggaagacgg ctgatgaatg
     5401 gtcaggatga cggtcttgcc cttctgcgac agcttcttca gcacctggac gacgctgtgg
     5461 gcggtaaagg agtccagtcc ggaggtgggc tcatcgcaga tcagaagcgg cggatcggtt
     5521 agagcctcgg aggcgaatgc cagacgcttc ctttctccgc cggacagacc tttcaccctg
     5581 ccgggcacac cgatgatcgt gtgctgacat ttgctgagcg aaagctcctg gatcacctga
     5641 tccacgcggg ccactcgctg ccgataggtc agatgtcgtg gcatccgcac catggcttgg
     5701 aaaatcaggt gttccctggc cgttagggag ccgataaaga ggtcatcctg ctggacatag
     5761 gcgcacctgg cctgcatctc cttggcgtcc acaggttggc cattgagcag tcgcatcccg
     5821 gatggcgata cttggatgcc ctgcggcgat cgaaaggcaa gggcattcag cagggtcgtc
     5881 tttccggcac cggaactgcc catcacggcc aaaagttcgc ccggataggc cacgccgcaa
     5941 actgagtttc aaattggtaa ttggaccctt tattaagatt tcacacagat cagccgactg
     6001 cgaatagaaa ctcaccgttc ttgagcaaat gtttcctggg cgccggtatg tgtcgctcgt
     6061 tgcagaatag tccgcgtgtc cggttgacca gctgccgcca tccggagccc ggctgattga
     6121 ccgccccaaa gatgtccata ttgtgccagg cataggtgag gttctcggct agttggccgc
     6181 tccctgaacc ggagtcctcc ggcggactgg gtggccggag cgtgccgtag tttttggcct
     6241 gcccgaagcc ctggttaatg cagctctgcg aagccgctcc gctgtcaccc tgcaatgata
     6301 ggggatctca aatatcaact acaagcgtta tgctcatcta accccgaaca aaaagtaccc
     6361 cgaagtatcc tacgaagtag gtttatactt ttatttattt tttgtgcatc taggatcagc
     6421 ttaaaatatc tggttgttat attttttgta aaaaagaata tagtcgaaaa tgaatgcctt
     6481 tagatgtctt gatcatgata tgatctcaaa aattgtctta tatagcgaga acagctacca
     6541 gaataatctg tttcgtgtca ctatttgttt gtgcaattgc ggtttgggat ttttgtgggt
     6601 cgcagttctc acgccgcaga caatttgatg ttgcaatcgc agttcctata gatcaagtga
     6661 acttaagatg tatgcacatg tactactcac attgttcaga tgctcggcag atgggtgttt
     6721 gctgcctccg cgaattaata gctcctgatc ctcttggccc attgccggga tttttcacac
     6781 tttcccctgc ttacccaccc aaaaccaatc accaccccaa tcactcaaaa aacaaacaaa
     6841 aataagaagc gagaggagtt ttggcacagc actttgtgtt taattgatgg cgtaaaccgc
     6901 ttggagcttc gtcacgaaac cgctgacaaa atgcaactga aggcggacat tgacgctacg
     6961 taacgctaca aacggtggcg aaagagatag cggacgcagc ggcgaaagag acggcgatat
     7021 ttctgtggac agagaaggag gcaaacagcg ctgactttga gtggaatgtc attttgagtg
     7081 agaggtaatc gaaagaacct ggtacatcaa atacccttgg atcgaagtaa atttaaaact
     7141 gatcagataa gttcaatgat atccagtgca gtaaaaaaaa aaaatgtttt ttttatctac
     7201 tttccgcaaa aatgggtttt attaacttac atacatacta ggcgcgccca tatgttcggc
     7261 ttgtcgacat gcccgccgtg accgtcgaga acccgctgac gctgccccgc gtatccgcac
     7321 ccgccgacgc cgtcgcacgt cccgtgctca ccgtgaccac cgcgcccagc ggtttcgagg
     7381 gcgagggctt cccggtgcgc cgcgcgttcg ccgggatcaa ctaccgccac ctcgacccgt
     7441 tcatcatgat ggaccagatg ggtgaggtgg agtacgcgcc cggggagccc aagggcacgc
     7501 cctggcaccc gcaccgcggc ttcgagaccg tgacctacat cgtcgacggt aacatgtgag
     7561 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7621 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7681 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7741 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7801 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7861 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7921 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7981 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8041 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8101 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8161 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8221 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8281 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8401 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9241 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9301 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9361 c
//
