LOCUS       sequence-219858-       10208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, 
            Rubin GM
  TITLE     Refinement of tools for targeted gene expression in Drosophila.
  JOURNAL   Genetics. 2010 Oct;186(2):735-55. doi: 10.1534/genetics.110.119917. 
            Epub 2010 Aug 9.
  PUBMED    20697123
REFERENCE   2  (bases 1 to 10208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.110.119917"; journalName: "Genetics"; date: 
            "2010-10"; volume: "186"; issue: "2"; pages: "735-55"
COMMENT     Sequence Label: pBPnlsLexA::p65Uw
FEATURES             Location/Qualifiers
     source          1..10208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     34..52
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    80..204
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(229..234,235..252,253..259)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             313..972
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(379..398)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1314..1619
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1498..1517
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1660..1784)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1980..2000
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2010..2261,2262..2615)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     CDS             3060..3431
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     primer_bind     complement(3700..3716)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3700..3716)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(4727..5341,5412..5543,5747..6062,6124..6778,
                     6853..7126,7528..7599))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     8051..8069
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    8294..8363
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(8450..9038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8530..8549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9209..10000,10001..10069))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9832..9851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10070..10174)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
       61 cttcaagaat tcgtttatca caagtttgta caaaaaagct gaacgagaaa cgtaaaatga
      121 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
      181 aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat
      241 gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct
      301 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      361 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      421 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      481 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
      541 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      601 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      661 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
      721 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
      781 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
      841 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
      901 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
      961 ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg
     1021 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     1081 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     1141 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     1201 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     1261 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt
     1321 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     1381 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     1441 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     1501 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     1561 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     1621 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
     1681 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
     1741 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgataaa cggccggccg
     1801 agctcgcccg gggatcgagc gcagcggtat aaaagggcgc ggggtggctg agagcatcag
     1861 ttgtgaatga atgttcgagc cgagcagacg tgccgctgcc ttcgttaata tcctttgaat
     1921 aagccaactt tgaatcacaa gacgcatacc aaacggtacc gctagcaatc aaaatgccac
     1981 ccaagaagaa gcgaaaagta gaagatccaa tgaaggctct cacggcccga caacaggaag
     2041 tttttgattt gatacgggat catatatccc aaacgggtat gcctccgacc cgcgcagaga
     2101 tagcacagcg actgggcttt cgatcgccta acgccgcgga ggagcacttg aaggcactgg
     2161 cccgcaaggg tgtcattgaa atcgtgtccg gtgcgagccg cggaatccgg ctgttgcagg
     2221 aggaggaaga gggcctgcca ctggtgggac gcgtggccgc tggcgagccg ctgctggccc
     2281 agcaacacat agagggacac tatcaggtgg acccctcctt gtttaagcca aatgctgatt
     2341 tcctgttgcg ggtgtcggga atgtccatga aggacatcgg tattatggat ggtgacctcc
     2401 tcgccgtcca taagacacag gatgtcagga acggccaggt agtcgttgcc aggatagacg
     2461 atgaggtcac tgtgaaacgt ctcaagaagc aaggcaataa ggtcgagctg ctgccggaga
     2521 atagcgagtt caagccgatc gtggtggatc tgcgacagca gtcctttact atcgagggct
     2581 tggccgtggg tgtgatccgc aacggagatt ggctgggatc cacgccgatg gagttccagt
     2641 acctgcccga tacggatgac cgtcaccgta tcgaagaaaa gcggaagcga acctatgaaa
     2701 ccttcaagtc catcatgaaa aagtccccct tctcgggccc cacggacccg cgccccccgc
     2761 cccgtcgtat tgcggttcct tcgcgcagca gtgccagcgt ccccaaaccc gcaccgcagc
     2821 cctacccgtt cacttcctcc cttagcacga ttaactatga tgagttcccc acgatggtgt
     2881 tccccagtgg acaaatttcc caggcatcgg cactggctcc ggccccaccg caagtcctcc
     2941 cccaggcgcc cgctccggca ccggctcccg caatggtgag tgctctggcc caggcccccg
     3001 ctccagtccc cgtgctggcg cctggacccc cacaggcagt tgcccctcct gctccgaaac
     3061 caacgcaggc gggcgaagga accctgagcg aggccctctt gcagcttcag ttcgatgacg
     3121 aagacttggg agccctgctg ggtaacagca cagaccctgc cgtattcacc gatctcgcat
     3181 ccgtggacaa cagcgagttt cagcagctct tgaatcaggg aatcccggtc gcacctcata
     3241 ccacagagcc catgctgatg gaatacccgg aggctatcac gcgactggtg accggcgcac
     3301 agcgaccacc cgatccagcc cctgccccac tgggtgcccc gggtttgccc aatggcctcc
     3361 tcagcggcga tgaggatttc tccagcatcg ctgatatgga tttctccgct ttgctgagcc
     3421 agataagctc ctaagtttaa acaagcttat cgataccgtc gactaaagcc aaatagaaaa
     3481 ttattcagtt cctggcttaa gtttttaaaa gtgatattat ttatttggtt gtaaccaacc
     3541 aaaagaatgt aaataactaa tacataatta tgttagtttt aagttagcaa caaattgatt
     3601 ttagctatat tagctacttg gttaataaat agaatatatt tatttaaaga taattgcgtt
     3661 tttattgtca gggagtgagt ttgcttaaaa actcgtttag atccactagt tctagagcgg
     3721 ccgctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg
     3781 gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg
     3841 ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc
     3901 gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga ggcgcgccct
     3961 agttccagtg aaatccaagc attttctaaa ttaaatgtat tcttattatt atagttgtta
     4021 tttttgatat atataaacaa cactattatg cccaccattt ttttgagatg catctacaca
     4081 aggaacaaac actggatgtc actttcagtt caaattgtaa cgctaatcac tccgaacagg
     4141 tcacaaaaaa ttaccttaaa aagtcataat attaaattag aataaatata gctgtgaggg
     4201 aaatatatac aaatatattg gagcaaataa attgtacata caaatattta ttactaattt
     4261 ctattgagac gaaatgaacc actcggaacc atttgagcga accgaatcgc gcggaactaa
     4321 cgacagtcgc tccaaggtcg tcgaacaaaa ggtgaatgtg ttgcggagag cgggtgggag
     4381 acagcgaaag agcaactacg aaacgtggtg tggtggaggt gaattatgaa gagggcgcgc
     4441 gatttgaaaa gtatgtatat aaaaaatata tcccggtgtt ttatgtagcg ataaacgagt
     4501 ttttgatgta aggtatgcag gtgtgtaagt cttttggtta gaagacaaat ccaaagtcta
     4561 cttgtgggga tgttcgaagg ggaaatactt gtattctata ggtcatatct tgtttttatt
     4621 ggcacaaata taattacatt agctttttga gggggcaata aacagtaaac acgatggtaa
     4681 taatggtaaa aaaaaaaaac aagcagttat ttcggatata tgtcggctac tccttgcgtc
     4741 gggcccgaag tcttagagcc agatatgcga gcacccggaa gctcacgatg agaatggcca
     4801 gacccacgta gtccagcggc agatcggcgg cggagaagtt aagcgtctcc aggatgacct
     4861 tgcccgaact ggggcacgtg gtgttcgacg atgtgcagct aatttcgccc ggctccacgt
     4921 ccgcccattg gttaatcagc agaccctcgt tggcgtaacg gaaccatgag aggtacgaca
     4981 accatttgag gtatactggc accgagcccg agttcaagaa gaagccgcca aagagcagga
     5041 atggtatgat aaccggcgga cccacagaca gcgccatcga ggtcgaggag ctggcgcagg
     5101 atattagata tccgaaggac gttgacacat tggccaccag agtgaccagc gccaggcagt
     5161 tgaagaagtg cagcactccg gcccgcagtc cgatcatcgg ataggcaatc gccgtgaaga
     5221 ccagtggcac tgtgagaaaa agcggcaatt cggcaatcgt tttgcccaga aagtatgtgt
     5281 cacagcgata aagtcgactt cgggcctccc tcataaaaac tggcagctct gaggtgaaca
     5341 cctaaatcga atcgattcat tagaaagtta gtaaattatt gaaatgcaaa tgtattctaa
     5401 acatgactta catttatcgt ggcaaagacg ttttgaaagg tcatgttggt caggaagagg
     5461 aagatggctc cgttgatatt catcacaccc acttgcgtga gttgttggcc caaaaagatg
     5521 aggccaatca agatggcaac catctgcaaa ttaaaatgtt actcgcatct cattaatatt
     5581 cgcgagttaa atgaaattta tttatcttct gcaaaactat aaactataca tctcattgaa
     5641 aaaaactaag aagggtgtgg aatcaggcaa ttctatctaa aatctagcga atttgtttcc
     5701 aagaattgta agcgttatat catttgtttc cactggaacc actcaccgtt gtctgaataa
     5761 gtcgcacttt tacgaggagt ggttccttga gcaccgacag ccaggatcgc cacaggaccg
     5821 cccggaactg catgaaccag gtggccttgt aggtgtaccc attctccggc tgctccagtg
     5881 gcttctccag atttttggtg gccaacaact gctccatatc ccgggctact ttgctaatgg
     5941 caaaattgtc gcatatcttg gcgatccgat cacgggactc gatctcccgt ccgggcacaa
     6001 cggccaacac ctgtacgtaa aagtccgccg gattgtagtt ggtaggacac tgggcaccca
     6061 cgctggatag gagttgagat gttatgtaat actagatacc cttaataaac acatcgaact
     6121 cactaggaaa agaagtcgac ggcttcgctg ggagtgccca agaaagctac cctgccctcg
     6181 gccatcagaa ggatcttgtc aaagagctca aacagctcgg aagacggctg atgaatggtc
     6241 aggatgacgg tcttgccctt ctgcgacagc ttcttcagca cctggacgac gctgtgggcg
     6301 gtaaaggagt ccagtccgga ggtgggctca tcgcagatca gaagcggcgg atcggttaga
     6361 gcctcggagg cgaatgccag acgcttcctt tctccgccgg acagaccttt caccctgccg
     6421 ggcacaccga tgatcgtgtg ctgacatttg ctgagcgaaa gctcctggat cacctgatcc
     6481 acgcgggcca ctcgctgccg ataggtcaga tgtcgtggca tccgcaccat ggcttggaaa
     6541 atcaggtgtt ccctggccgt tagggagccg ataaagaggt catcctgctg gacataggcg
     6601 cacctggcct gcatctcctt ggcgtccaca ggttggccat tgagcagtcg catcccggat
     6661 ggcgatactt ggatgccctg cggcgatcga aaggcaaggg cattcagcag ggtcgtcttt
     6721 ccggcaccgg aactgcccat cacggccaaa agttcgcccg gataggccac gccgcaaact
     6781 gagtttcaaa ttggtaattg gaccctttat taagatttca cacagatcag ccgactgcga
     6841 atagaaactc accgttcttg agcaaatgtt tcctgggcgc cggtatgtgt cgctcgttgc
     6901 agaatagtcc gcgtgtccgg ttgaccagct gccgccatcc ggagcccggc tgattgaccg
     6961 ccccaaagat gtccatattg tgccaggcat aggtgaggtt ctcggctagt tggccgctcc
     7021 ctgaaccgga gtcctccggc ggactgggtg gccggagcgt gccgtagttt ttggcctgcc
     7081 cgaagccctg gttaatgcag ctctgcgaag ccgctccgct gtcaccctgc aatgataggg
     7141 gatctcaaat atcaactaca agcgttatgc tcatctaacc ccgaacaaaa agtaccccga
     7201 agtatcctac gaagtaggtt tatactttta tttatttttt gtgcatctag gatcagctta
     7261 aaatatctgg ttgttatatt ttttgtaaaa aagaatatag tcgaaaatga atgcctttag
     7321 atgtcttgat catgatatga tctcaaaaat tgtcttatat agcgagaaca gctaccagaa
     7381 taatctgttt cgtgtcacta tttgtttgtg caattgcggt ttgggatttt tgtgggtcgc
     7441 agttctcacg ccgcagacaa tttgatgttg caatcgcagt tcctatagat caagtgaact
     7501 taagatgtat gcacatgtac tactcacatt gttcagatgc tcggcagatg ggtgtttgct
     7561 gcctccgcga attaatagct cctgatcctc ttggcccatt gccgggattt ttcacacttt
     7621 cccctgctta cccacccaaa accaatcacc accccaatca ctcaaaaaac aaacaaaaat
     7681 aagaagcgag aggagttttg gcacagcact ttgtgtttaa ttgatggcgt aaaccgcttg
     7741 gagcttcgtc acgaaaccgc tgacaaaatg caactgaagg cggacattga cgctacgtaa
     7801 cgctacaaac ggtggcgaaa gagatagcgg acgcagcggc gaaagagacg gcgatatttc
     7861 tgtggacaga gaaggaggca aacagcgctg actttgagtg gaatgtcatt ttgagtgaga
     7921 ggtaatcgaa agaacctggt acatcaaata cccttggatc gaagtaaatt taaaactgat
     7981 cagataagtt caatgatatc cagtgcagta aaaaaaaaaa atgttttttt tatctacttt
     8041 ccgcaaaaat gggttttatt aacttacata catactaggc gcgcccatat gttcggcttg
     8101 tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct gccccgcgta tccgcacccg
     8161 ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc gcccagcggt ttcgagggcg
     8221 agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta ccgccacctc gacccgttca
     8281 tcatgatgga ccagatgggt gaggtggagt acgcgcccgg ggagcccaag ggcacgccct
     8341 ggcacccgca ccgcggcttc gagaccgtga cctacatcgt cgacggtaac atgtgagcaa
     8401 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8461 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8521 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8581 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8641 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8701 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8761 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8821 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8881 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8941 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9001 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9061 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9121 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9181 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9241 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9301 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9361 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9421 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9481 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9541 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9601 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9661 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9721 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9781 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9841 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9901 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9961 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10021 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10081 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10141 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    10201 acgtctaa
//
