LOCUS       sequence-219861-        3890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is part of the Minimal Antibiotic Resistance Platform 
            (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that 
            constitutively expresses genes using the Plac promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Cox G, Sieron A, King AM, De Pascale G, Pawlowski AC, Koteva K, 
            Wright GD
  TITLE     A Common Platform for Antibiotic Dereplication and Adjuvant 
            Discovery.
  JOURNAL   Cell Chem Biol. 2017 Jan 19;24(1):98-109. doi: 
            10.1016/j.chembiol.2016.11.011. Epub 2016 Dec 22.
  PUBMED    28017602
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.11.011"; journalName: "Cell Chem Biol"; 
            date: "2017-01-19- 19"; volume: "24"; issue: "1"; pages: "98-109"
COMMENT     Sequence Label: pGDP4 ermC
FEATURES             Location/Qualifiers
     source          1..3890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             185..207
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             226..243
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             253..270
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1015..1032
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(1094..1121)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1146..1250
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1251..1319,1320..2111)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1469..1488)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2282..2870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2771..2790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3024..3041
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3056..3195
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3121..3140)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3281..3303
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3297..3488)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 agtaaggcaa ccccgccagc ctagccggga gatctgcggc ggcggtgctc aacggcctca
       61 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgagatcccg
      121 gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggtctagaaa
      181 taattttgtt taactttaag aaggagatat accatgggca gcagccatca tcatcatcat
      241 cacagcagcg gcctggtgcc gcgcggcagc catatgaacg aaaaaaacat caaacactcg
      301 caaaacttta tcacctcaaa acacaacatc gacaaaatta tgacgaacat ccgcctgaat
      361 gaacatgata acatttttga aatcggcagc ggtaaaggcc acttcaccct ggaactggtc
      421 cagcgttgca actttgtgac ggcgattgaa atcgatcata aactgtgtaa aaccacggaa
      481 aataaactgg ttgatcacga caacttccaa gtcctgaata aagacattct gcagttcaaa
      541 ttcccgaaaa accaaagtta caaaatcttc ggtaacatcc cgtacaatat tagcaccgat
      601 attatccgca aaatcgtctt cgattctatt gcagacgaaa tttatctgat cgttgaatac
      661 ggctttgcta aacgtctgct gaatacgaaa cgctcactgg cgctgttcct gatggccgaa
      721 gtggatatta gtatcctgtc catggttccg cgtgaatatt ttcatccgaa accgaaagtg
      781 aacagctctc tgatccgtct gaatcgcaaa aaatcacgca tttcgcacaa agacaaacag
      841 aaatacaact acttcgtgat gaaatgggtt aacaaagaat acaagaaaat tttcaccaaa
      901 aaccaattca acaattccct gaaacatgca ggtattgatg acctgaataa tatctccttc
      961 gaacaattcc tgagcctgtt caactcctac aaactgttca ataaataact cgagcaccac
     1021 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
     1081 gccaccgctg agcaaaaggc catccgtcag gatggccttc tggtggcact tttcggggaa
     1141 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1201 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1261 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1321 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1381 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1441 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     1501 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1561 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1621 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1681 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1741 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     1801 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1861 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     1921 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     1981 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2041 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2101 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2161 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2221 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2281 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2341 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2401 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     2461 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2521 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2581 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2641 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2701 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2761 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2821 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2881 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2941 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3001 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     3061 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag
     3121 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     3181 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     3241 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     3301 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
     3361 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
     3421 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
     3481 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg
     3541 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa
     3601 cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag
     3661 ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat
     3721 cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt
     3781 tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag
     3841 cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
//
