LOCUS       sequence-219870-        3566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is part of the Minimal Antibiotic Resistance Platform 
            (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that 
            constitutively expresses genes using the Pbla promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Cox G, Sieron A, King AM, De Pascale G, Pawlowski AC, Koteva K, 
            Wright GD
  TITLE     A Common Platform for Antibiotic Dereplication and Adjuvant 
            Discovery.
  JOURNAL   Cell Chem Biol. 2017 Jan 19;24(1):98-109. doi: 
            10.1016/j.chembiol.2016.11.011. Epub 2016 Dec 22.
  PUBMED    28017602
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.11.011"; journalName: "Cell Chem Biol"; 
            date: "2017-01-19- 19"; volume: "24"; issue: "1"; pages: "98-109"
COMMENT     Sequence Label: pGDP3 rmtB
FEATURES             Location/Qualifiers
     source          1..3566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             232..254
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             273..290
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             300..317
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             691..708
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(770..797)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        822..926
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(927..995,996..1787)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1145..1164)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1958..2546
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2447..2466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2700..2717
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2732..2871
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2797..2816)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2957..2979
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2973..3164)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 agtaaggcaa ccccgccagc ctagccggga gatctggcct attggttaaa aaatgagctg
       61 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
      121 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      181 tgtatccgct catgagacaa taaccctgat aaatgcttct ctagaaataa ttttgtttaa
      241 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc
      301 tggtgccgcg cggcagccat atggctagca tgaacatcaa cgatgccctc acctccatcc
      361 tggcctcaaa aaaataccgc gccctttgcc ctgcaggatg tgctgtgtgc gccgcccgcc
      421 gaagccggcg acctggcgct gatttttaag cttttgcccc tgctggagcg ggagcaggcc
      481 ggttctgcca tggcactttt acaatccctc aataccccgc gcatggctgt cagctttccc
      541 acgcgtagtt taggcgggcg tggaaaaggc atggaggcga actacgccgc atggttcgag
      601 ggcggcttgc ccgccgagtt tgagattgag gataaaaaga ccatcggaac agaacttata
      661 tacttgataa aaaagaatgg ataactcgag caccaccacc accaccactg agatccggct
      721 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca aaaggccatc
      781 cgtcaggatg gccttctggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      841 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      901 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      961 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1021 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1081 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     1141 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     1201 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1261 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1321 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1381 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1441 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat
     1501 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1561 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1621 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1681 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1741 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1801 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1861 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1921 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1981 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2041 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2101 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2161 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2221 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2281 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2341 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2401 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2461 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2521 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2581 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2641 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2701 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2761 tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca atctgctctg atgccgcata
     2821 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     2881 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     2941 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     3001 cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct
     3061 gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg
     3121 ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa
     3181 gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata
     3241 cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg
     3301 gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat
     3361 acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata
     3421 atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc
     3481 attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg
     3541 cgtatcggtg attcattctg ctaacc
//
