LOCUS       sequence-219877-       10182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Sif S, Saurin AJ, Imbalzano AN, Kingston RE
  TITLE     Purification and characterization of mSin3A-containing Brg1 and hBrm
            chromatin remodeling complexes.
  JOURNAL   Genes Dev 2001 Mar 1;15(5):603-18.
  PUBMED    11238380
REFERENCE   2  (bases 1 to 10182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev";
            date: "2001-03-1"; volume: "15(5)"; pages: "603-18"
COMMENT     Sequence Label: pBABE BRG1
FEATURES             Location/Qualifiers
     source          1..10182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             19..488
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    553..752
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             953..1369
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1299..1321
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1337..1353
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     intron          1445..1541
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             6482..6505
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(6523..6543)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6528..6857
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6708..6843
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6867..7466
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6867..6886)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7363..7383
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7687..8156
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8286..8303)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8457..9045)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8537..8556)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9205..9996,9997..10065))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9828..9847
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10066..10170)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaagtgcca cctgcagcct gaatatgggc caaacaggat atctgtggta agcagttcct
       61 gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg
      121 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca
      181 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
      241 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
      301 ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga
      361 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
      421 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
      481 gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca
      541 ccaccgggag gcaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat
      601 gactgatttt atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac
      661 ccgtggtgga actgacgagt tctgaacacc cggccgcaac cctgggagac gtcccaggga
      721 ctttgggggc cgtttttgtg gcccgacctg aggaagggag tcgatgtgga atccgacccc
      781 gtcaggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc ctccgtctga
      841 atttttgctt tcggtttgga accgaagccg cgcgtcttgt ctgctgcagc gctgcagcat
      901 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac
      961 tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca
     1021 caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc
     1081 aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt
     1141 taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt
     1201 gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa
     1261 gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac
     1321 cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccg gccggatccc
     1381 agtgtggtgg tacgtaggaa ttcgccagca cagtggtcga cggtatcgat aagcttgata
     1441 actggtaagt ttagtctttt tgtcttttat ttcaggtccc ggatccggtg gtggtgcaaa
     1501 tcaaagaact gctcctcagt ggatgttgcc tttacttcta ggcctgtacg gaagtgttac
     1561 ttctgctcta aaagctgctc tagccctcga cggcgccggc ggctcctgca ggaggccact
     1621 gtctgcagct cccgtgaaga tgtccactcc agacccaccc ctgggcggaa ctcctcggcc
     1681 aggtccttcc ccgggccctg gcccttcccc tggagccatg ctgggcccta gcccgggtcc
     1741 ctcgccgggc tccgcccaca gcatgatggg gcccagccca gggccgccct cagcaggaca
     1801 ccccatcccc acccaggggc ctggagggta ccctcaggac aacatgcacc agatgcacaa
     1861 gcccatggag tccatgcatg agaagggcat gtcggacgac ccgcgctaca accagatgaa
     1921 aggaatgggg atgcggtcag ggggccatgc tgggatgggg cccccgccca gccccatgga
     1981 ccagcactcc caaggttacc cctcgcccct gggtggctct gagcatgcct ctagtccagt
     2041 tccagccagt ggcccgtctt cggggcccca gatgtcttcc gggccaggag gtgccccgct
     2101 ggatggtgct gacccccagg ccttggggca gcagaaccgg ggcccaaccc catttaacca
     2161 gaaccagctg caccagctca gagctcagat catggcctac aagatgctgg ccagggggca
     2221 gcccctcccc gaccacctgc agatggcggt gcagggcaag cggccgatgc ccgggatgca
     2281 gcagcagatg ccaacgctac ctccaccctc ggtgtccgca acaggacccg gccctggccc
     2341 tggccctggc cccggcccgg gtcccggccc ggcacctcca aattacagca ggcctcatgg
     2401 tatgggaggg cccaacatgc ctcccccagg accctcgggc gtgccccccg ggatgccagg
     2461 ccagcctcct ggagggcctc ccaagccctg gcctgaagga cccatggcga atgctgctgc
     2521 ccccacgagc acccctcaga agctgattcc cccgcagcca acgggccgcc cttcccccgc
     2581 gccccctgcc gtcccacccg ccgcctcgcc cgtgatgcca ccgcagaccc agtcccccgg
     2641 gcagccggcc cagcccgcgc ccatggtgcc actgcaccag aagcagagcc gcatcacccc
     2701 catccagaag ccgcggggcc tcgaccctgt ggagatcctg caggagcgcg agtacaggct
     2761 gcaggctcgc atcgcacacc gaattcagga acttgaaaac cttcccgggt ccctggccgg
     2821 ggatttgcga accaaagcga ccattgagct caaggccctc aggctgctga acttccagag
     2881 gcagctgcgc caggaggtgg tggtgtgcat gcggagggac acagcgctgg agacagccct
     2941 caatgctaag gcctacaagc gcagcaagcg ccagtccctg cgcgaggccc gcatcactga
     3001 gaagctggag aagcagcaga agatcgagca ggagcgcaag cgccggcaga agcaccagga
     3061 atacctcaat agcattctcc agcatgccaa ggatttcaag gaatatcaca gatccgtcac
     3121 aggcaaaatc cagaagctga ccaaggcagt ggccacgtac catgccaaca cggagcggga
     3181 gcagaagaaa gagaacgagc ggatcgagaa ggagcgcatg cggaggctca tggctgaaga
     3241 tgaggagggg taccgcaagc tcatcgacca gaagaaggac aagcgcctgg cctacctctt
     3301 gcagcagaca gacgagtacg tggctaacct cacggagctg gtgcggcagc acaaggctgc
     3361 ccaggtcgcc aaggagaaaa agaagaaaaa gaaaaagaag aaggcagaaa atgcagaagg
     3421 acagacgcct gccattgggc cggatggcga gcctctggac gagaccagcc agatgagcga
     3481 cctcccggtg aaggtgatcc acgtggagag tgggaagatc ctcacaggca cagatgcccc
     3541 caaagccggg cagctggagg cctggctcga gatgaacccg gggtatgaag tagctccgag
     3601 gtctgatagt gaagaaagtg gctcagaaga agaggaagag gaggaggagg aagagcagcc
     3661 gcaggcagca cagcctccca ccctgcccgt ggaggagaag aagaagattc cagatccaga
     3721 cagcgatgac gtctctgagg tggacgcgcg gcacatcatt gagaatgcca agcaagatgt
     3781 cgatgatgaa tatggcgtgt cccaggccct tgcacgtggc ctgcagtcct actatgccgt
     3841 ggcccatgct gtcactgaga gagtggacaa gcagtcagcg cttatggtca atggtgtcct
     3901 caaacagtac cagatcaaag gtttggagtg gctggtgtcc ctgtacaaca acaacctgaa
     3961 cggcatcctg gccgacgaga tgggcctggg gaagaccatc cagaccatcg cgctcatcac
     4021 gtacctcatg gagcacaaac gcatcaatgg gcccttcctc atcatcgtgc ctctctcaac
     4081 gctgtccaac tgggcgtacg agtttgacaa gtgggccccc tccgtggtga aggtgtctta
     4141 caagggatcc ccagcagcaa gacgggcctt tgtcccccag ctccggagtg ggaagttcaa
     4201 cgtcttgctg acgacgtacg agtacatcat caaagacaag cacatcctcg ccaagatccg
     4261 ttggaagtac atgattgtgg acgaaggtca ccgcatgaag aaccaccact gcaagctgac
     4321 gcaggtgctc aacacgcact atgtggcacc ccgccgcctg ctgctgacgg gcacaccgct
     4381 gcagaacaag cttcccgagc tctgggcgct gctcaacttc ctgctgccca ccatcttcaa
     4441 gagctgcagc accttcgagc agtggtttaa cgcacccttt gccatgaccg gggaaaaggt
     4501 ggacctgaat gaggaggaaa ccattctcat catccggcgt ctccacaaag tgctgcggcc
     4561 cttcttgctc cgacgactca agaaggaagt cgaggcccag ttgcccgaaa aggtggagta
     4621 cgtcatcaag tgcgacatgt ctgcgctgca gcgagtgctc taccgccaca tgcaggccaa
     4681 gggcgtgctg ctgactgatg gctccgagaa ggacaagaag ggcaaaggcg gcaccaagac
     4741 cctgatgaac accatcatgc agctgcggaa gatctgcaac cacccctaca tgttccagca
     4801 catcgaggag tccttttccg agcacttggg gttcactggc ggcattgtcc aagggctgga
     4861 cctgtaccga gcctcgggta aatttgagct tcttgataga attcttccca aactccgagc
     4921 aaccaaccac aaagtgctgc tgttctgcca aatgacctcc ctcatgacca tcatggaaga
     4981 ttactttgcg tatcgcggct ttaaatacct caggcttgat ggaaccacga aggcggagga
     5041 ccggggcatg ctgctgaaaa ccttcaacga gcccggctct gagtacttca tcttcctgct
     5101 cagcacccgg gctggggggc tcggcctgaa cctccagtcg gcagacactg tgatcatttt
     5161 tgacagcgac tggaatcctc accaggacct gcaagcgcag gaccgagccc accgcatcgg
     5221 gcagcagaac gaggtgcgtg tgctccgcct ctgcaccgtc aacagcgtgg aggagaagat
     5281 cctagctgca gccaagtaca agctcaacgt ggaccagaag gtgatccagg ccggcatgtt
     5341 cgaccagaag tcctccagcc atgagcggcg cgccttcctg caggccatcc tggagcacga
     5401 ggagcaggat gaggaggaag acaaggtgcc cgacgacgag accgtcaacc agatgatcgc
     5461 ccggcacgag gaggagtttg atctgttcat gcgcatggac ctggaccgca ggcgcgagga
     5521 ggcccgcaac cccaagcgga agccgcgcct catggaggag gacgagctcc cctcgtggat
     5581 catcaaggac gacgcggagg tggagcggct gacctgtgag gaggaggagg agaagatgtt
     5641 cggccgtggc tcccgccacc gcaaggaggt ggactacagc gactcactga cggagaagca
     5701 gtggctcaag gccatcgagg agggcacgct ggaggagatc gaagaggagg tccggcagaa
     5761 gaaatcatca cggaagcgca agcgagacag cgacgccggc tcctccaccc cgaccaccag
     5821 cacccgcagc cgcgacaagg acgacgagag caagaagcag aagaagcgcg ggcggccgcc
     5881 tgccgagaaa ctctccccta acccacccaa cctcaccaag aagatgaaga agattgtgga
     5941 tgccgtgatc aagtacaagg acagcagcag tggacgtcag ctcagcgagg tcttcatcca
     6001 gctgccctcg cgaaaggagc tgcccgagta ctacgagctc atccgcaagc ccgtggactt
     6061 caagaagata aaggagcgca ttcgcaacca caagtaccgc agcctcaacg acctagagaa
     6121 ggacgtcatg ctcctgtgcc agaacgcaca gaccttcaac ctggagggct ccctgatcta
     6181 tgaagactcc atcgtcttgc agtcggtctt caccagcgtg cggcagaaaa tcgagaagga
     6241 ggatgacagt gaaggcgagg agagtgagga ggaggaagag ggcgaggagg aaggctccga
     6301 atccgaatct cggtccgtca aagtgaagat caagcttggc cggaaggaga aggcacagga
     6361 ccagctgaag ggcggccggc ggcggccgag ccgagggtcc cgagccaagc cggtcgtgag
     6421 tgacgatgac agtgaggagg aacaagagga ggaccgctca ggaagtggca gcgaagaaga
     6481 cgactacaag gacgacgatg acaaatgata agtcgaccct gtggaatgtg tgtcagttag
     6541 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6601 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6661 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     6721 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6781 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6841 gcctaggctt ttgcaaaaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     6901 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     6961 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     7021 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     7081 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     7141 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     7201 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     7261 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     7321 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     7381 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     7441 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     7501 ggagcgcacg accccatgca tcgataaaat aaaagatttt atttagtctc cagaaaaagg
     7561 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     7621 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
     7681 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     7741 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     7801 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
     7861 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     7921 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     7981 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
     8041 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     8101 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg
     8161 cagcatgtat caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag
     8221 ttgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8281 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8341 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8401 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8461 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8521 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8581 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8641 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8701 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8761 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8821 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8881 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8941 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9001 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9061 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9121 agattatcaa aaaggatctt cacctagatc cttttgcggc cgcaaatcaa tctaaagtat
     9181 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9241 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9301 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9361 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9421 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9481 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9541 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9601 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9661 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9721 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9781 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9841 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9901 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9961 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10021 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10081 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10141 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cg
//
