LOCUS       sequence-219887-        3877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is part of the Minimal Antibiotic Resistance Platform 
            (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that 
            constitutively expresses genes using the Pbla promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Cox G, Sieron A, King AM, De Pascale G, Pawlowski AC, Koteva K, 
            Wright GD
  TITLE     A Common Platform for Antibiotic Dereplication and Adjuvant 
            Discovery.
  JOURNAL   Cell Chem Biol. 2017 Jan 19;24(1):98-109. doi: 
            10.1016/j.chembiol.2016.11.011. Epub 2016 Dec 22.
  PUBMED    28017602
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.11.011"; journalName: "Cell Chem Biol"; 
            date: "2017-01-19- 19"; volume: "24"; issue: "1"; pages: "98-109"
COMMENT     Sequence Label: pGDP3 cat
FEATURES             Location/Qualifiers
     source          1..3877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             232..254
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             273..290
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             300..317
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1002..1019
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(1081..1108)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1133..1237
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1238..1306,1307..2098)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1456..1475)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2269..2857
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2758..2777
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3011..3028
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3043..3182
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3108..3127)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3268..3290
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3284..3475)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 agtaaggcaa ccccgccagc ctagccggga gatctggcct attggttaaa aaatgagctg
       61 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
      121 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      181 tgtatccgct catgagacaa taaccctgat aaatgcttct ctagaaataa ttttgtttaa
      241 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc
      301 tggtgccgcg cggcagccat atgaaatttc agcgaatcga tctagataat tggagcagaa
      361 ggtcttattt cgagcattat ctgaaccgag tgaattgcac cttcagtatg actgccaaca
      421 tagatataac ggaattgctg cccgctctac ggcaaaagga gatgaagctg tacccggctt
      481 ttctgtatat ggtgacaaac gctgtcaatg cgcatcgtga atttcggact tccttccatg
      541 cggatggcga gttgggctat tgggagagca tgatacccag ctatactttt tttcaccagg
      601 atgatcaaac tttttctacg atgtggacgg aattcgctga cgagttccct gtgttttatc
      661 aaaattacgt agcggatatg aaaaagtatg gggacaacaa aggtcttgtg gcaaaagagt
      721 tggaaccgcc ttacactttc cctgtctcgt gtattccctg ggtgagcttc agtgggttta
      781 atctgaatat ttcgggagat gggcgatatt tgctgccaat cattacgagc ggaaagtatt
      841 tcgggcaaga aggcaaaacg ttattgcctg tttctttgca ggtccatcat gcggtttgtg
      901 atggctatca cgcgagcctg ttcatccatg atctgcaaaa atgggcaaca aactataagg
      961 aatggttagg tgtcgagtga aagcttgcgg ccgcactcga gcaccaccac caccaccact
     1021 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     1081 aaaaggccat ccgtcaggat ggccttctgg tggcactttt cggggaaatg tgcgcggaac
     1141 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1201 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     1261 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     1321 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     1381 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     1441 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     1501 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     1561 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     1621 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     1681 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     1741 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
     1801 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     1861 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     1921 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     1981 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     2041 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     2101 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     2161 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     2221 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2281 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2341 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2401 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2461 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2521 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2581 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2641 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2701 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2761 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2821 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2881 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2941 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3001 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     3061 ccttacgcat ctgtgcggta tttcacaccg caatggtgca ctctcagtac aatctgctct
     3121 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     3181 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     3241 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     3301 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt
     3361 cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg
     3421 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
     3481 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga
     3541 tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta
     3601 aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc
     3661 gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga
     3721 tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga
     3781 aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc
     3841 acgttcgctc gcgtatcggt gattcattct gctaacc
//
