LOCUS       sequence-219893-        7760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Kopec CD, Li B, Wei W, Boehm J, Malinow R
  TITLE     Glutamate receptor exocytosis and spine enlargement during 
            chemically induced long-term potentiation.
  JOURNAL   J Neurosci. 2006 Feb 15. 26(7):2000-9.
  PUBMED    16481433
REFERENCE   2  (bases 1 to 7760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2006 Feb 15. 26(7):2000-9."
COMMENT     Sequence Label: pCI-SEP-GluR1
FEATURES             Location/Qualifiers
     source          1..7760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        85..464
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        465..668
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     619..639
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     665..689
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          804..936
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     919..938
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        981..999
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1148..1159
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1290..2000
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1317..1345)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1940..1960
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    4821..4942
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4832..4851)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4886..4905
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5123..5578
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5210..5229)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5420..5441
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5645..5664)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5764..5786
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5824..5842)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5910..6014
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6015..6083,6084..6875)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6233..6252)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7046..7634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7535..7554
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
       61 catgtccaat atgaccgcca tgttggcatt gattattgac tagttattaa tagtaatcaa
      121 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      181 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      241 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      301 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg
      361 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
      421 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      481 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      541 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      601 ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      661 gcagagctcg tttagtgaac cgtcagatca ctagaagctt tattgcggta gtttatcaca
      721 gttaaattgc taacgcagtc agtgcttctg acacaacagt ctcgaactta agctgcagaa
      781 gttggtcgtg aggcactggg caggtaagta tcaaggttac aagacaggtt taaggagacc
      841 aatagaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag gcacctattg
      901 gtcttactga catccacttt gcctttctct ccacaggtgt ccactcccag ttcaattaca
      961 gctcttaagg ctagagtact taatacgact cactataggc tagtggatcc cccgggctgc
     1021 aggaattcgg cacgagctcg gctccccttc caagagaaac aagagaaacc tcacggaagg
     1081 aagggaggaa ggaaagaagc aagcaaggaa ctgcaggaag aaaagagccg gcagagcatc
     1141 aagaagaatc gaagggaggg gagggaagac caaatctatg gttggaccag ggcttctttt
     1201 tcgccaatgt aaaaaggaat atgccgtaca tctttgcctt tttctgcacc ggttttctag
     1261 gtgcggttgt gggtgccaat ttccctagga gtaaaggaga agaacttttc actggagttg
     1321 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
     1381 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
     1441 aactacctgt tccttggcca acacttgtca ctactttaac ttatggtgtt caatgctttt
     1501 caagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt
     1561 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
     1621 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
     1681 aagatggaaa cattcttgga cacaaattgg aatacaacta taacgatcac caggtgtaca
     1741 tcatggcaga caaacaaaag aatggaatca aagctaactt caaaattaga cacaacattg
     1801 aagatggagg cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatgggc
     1861 ccgtcctttt accagacaac cattacctgt ttacaacttc tactctttcg aaagatccca
     1921 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
     1981 gcatggatga actatacaaa gctagcccca acaatatcca gatagggggg ttatttccaa
     2041 accaacaatc acaggaacat gcggctttta ggtttgcttt gtcacaactc acggagcccc
     2101 ccaagctgct tccccagatc gatattgtga acatcagcga cagctttgag atgacttacc
     2161 gtttctgttc ccagttctcc aaaggagtct atgccatctt tggattttat gaacgaagga
     2221 ctgtcaacat gctgacctcc ttctgtgggg ccctccatgt gtgcttcatt actccaagtt
     2281 ttcctgttga cacatccaat caatttgtcc ttcagctacg cccggaacta caggaagctc
     2341 tcattagcat tatcgaccat tacaagtggc aaacctttgt ctacatttat gatgctgacc
     2401 ggggcctgtc agtcctgcag agagtcttgg atacagccgc agagaagaac tggcaggtaa
     2461 cggctgtcaa cattctgaca accaccgagg aaggataccg gatgctcttt caggacctgg
     2521 agaagaaaaa ggagaggctg gtggtggttg actgtgaatc agaacgcctc aacgccatcc
     2581 tgggccagat cgtgaagcta gaaaagaatg gcatcgggta ccactacatc ctcgccaatc
     2641 tgggcttcat ggacattgac ttaaataagt tcaaggagag cggagccaat gtgacaggtt
     2701 tccagctggt gaactacaca gacacgatcc cagccagaat catgcagcaa tggaggacaa
     2761 gtgactcccg agaccatacc agggtggact ggaagaggcc aaagtacact tctgctctca
     2821 cctatgatgg tgtcaaggtg atggctgagg ccttccaaag cctgcggagg cagaggattg
     2881 acatatcccg ccgggggaat gctggggact gtctggctaa cccagctgtg ccctggggtc
     2941 aagggatcga catccagaga gccctgcagc aggtgcgctt cgaaggtttg acaggaaatg
     3001 tgcagttcaa cgagaaaggg cgccggacca attacaccct ccacgtgatc gaaatgaaac
     3061 atgatggaat ccgaaagatt ggttactgga atgaagacga taaatttgtc cccgcagcca
     3121 ccgacgctca ggctggaggg gacaactcaa gcgtccagaa taggacctac atcgtcacta
     3181 ctatcctcga agatccttac gtgatgctta aaaagaatgc caaccagttt gagggcaatg
     3241 accgctatga gggctactgt gtggagctgg ctgcagagat cgccaagcac gtgggctact
     3301 cctaccgact tgagattgtc agcgacggca aatatggagc ccgggatccc gacacaaagg
     3361 cttggaatgg catggtggga gaactggtct atggaagagc agacgtggct gtggctccct
     3421 tgaccataac cttggtccgg gaggaagtca tcgacttctc caagccattc atgagtttgg
     3481 gaatctccat tatgattaag aagccacaga agtccaagcc aggtgtcttc tcctttcttg
     3541 accctttggc ctatgagatc tggatgtgta tagtgtttgc ctacattgga gtgagcgtcg
     3601 tcctcttcct ggtcagccgt ttcagcccct acgaatggca cagcgaagag tttgaagagg
     3661 gacgagacca gacaaccagt gaccagtcaa atgagtttgg catattcaac agcctgtggt
     3721 tctccctggg ggccttcatg cagcaaggat gtgacatttc ccccaggtcc ctgtccggac
     3781 gcatcgtcgg cggcgtctgg tggttcttca ctttgatcat catctcctcg tacacagcca
     3841 acctggctgc cttcctgact gtggagagga tggtgtctcc cattgagagt gcagaggacc
     3901 tggcaaagca gacggaaatt gcttatggga cattggaagc aggctccact aaggagttct
     3961 tcaggagatc taaaatcgct gtgtttgaga agatgtggac atacatgaag tctgcagaac
     4021 catccgtgtt tgttcggacc acagaggaag gcatgatcag agtgagaaaa tctaaaggca
     4081 aatacgccta cctcctggag tccaccatga atgagtatat tgagcaacga aagccctgtg
     4141 acaccatgaa agtgggaggt aacttggatt ccaaaggcta tggcattgcg acacccaagg
     4201 ggtccgccct gagaaatcca gtaaacctgg cagtgttaaa actgaacgag caggggcttt
     4261 tggacaaatt gaaaaacaaa tggtggtacg acaagggcga gtgcggcagc gggggaggtg
     4321 actccaagga caagaccagc gctttgagcc tcagcaatgt ggcaggcgtg ttctacatcc
     4381 tgattggagg gctgggactg gccatgctgg ttgccttaat cgagttctgc tacaaatccc
     4441 gtagcgagtc gaagcggatg aagggtttct gtttgatccc acagcaatcc atcaatgaag
     4501 ccatacggac atcgaccctc ccccggaaca gtggggcagg agccagcgga ggaggcggca
     4561 gtggagagaa tggccgggtg gtcagccagg acttccccaa gtccatgcaa tccattccct
     4621 gcatgagtca cagttcaggg atgcccttgg gagccacagg attgtaactg gagcagacag
     4681 gaaacccttg gggagcaggc tcaggcttcc acagccccat cccaagccct tcagtgccaa
     4741 aaaaaaaaaa aaaaaaaact cgagaattca cgcgtggtac ctctagagtc gacccgggcg
     4801 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     4861 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     4921 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     4981 gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga
     5041 taaggatccg ggctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     5101 gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5161 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5221 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5281 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5341 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5401 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5461 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5521 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
     5581 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     5641 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     5701 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     5761 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     5821 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     5881 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5941 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6001 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6061 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6121 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6181 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6241 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6301 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6361 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6421 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6481 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6541 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6601 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6661 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6721 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6781 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6841 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6901 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6961 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7021 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7081 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7141 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7201 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7261 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7321 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7381 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7441 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7501 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7561 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7621 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7681 cttttgctca catggctcga cagatcttca atattggcca ttagccatat tattcattgg
     7741 ttatatagca taaatcaata
//
