LOCUS       sequence-219894-        4360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is part of the Minimal Antibiotic Resistance Platform 
            (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that 
            constitutively expresses genes using the Plac promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Cox G, Sieron A, King AM, De Pascale G, Pawlowski AC, Koteva K, 
            Wright GD
  TITLE     A Common Platform for Antibiotic Dereplication and Adjuvant 
            Discovery.
  JOURNAL   Cell Chem Biol. 2017 Jan 19;24(1):98-109. doi: 
            10.1016/j.chembiol.2016.11.011. Epub 2016 Dec 22.
  PUBMED    28017602
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.11.011"; journalName: "Cell Chem Biol"; 
            date: "2017-01-19- 19"; volume: "24"; issue: "1"; pages: "98-109"
COMMENT     Sequence Label: pGDP4 tet(A)
FEATURES             Location/Qualifiers
     source          1..4360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             185..207
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             226..243
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             253..270
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1485..1502
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(1564..1591)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1616..1720
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1721..1789,1790..2581)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1939..1958)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2752..3340
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3241..3260
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3494..3511
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3526..3665
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3591..3610)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3751..3773
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3767..3958)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 agtaaggcaa ccccgccagc ctagccggga gatctgcggc ggcggtgctc aacggcctca
       61 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgagatcccg
      121 gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggtctagaaa
      181 taattttgtt taactttaag aaggagatat accatgggca gcagccatca tcatcatcat
      241 cacagcagcg gcctggtgcc gcgcggcagc catatgaact cgtcaaccaa aattgctctt
      301 gttatcactt tacttgatgc tatggggatt ggcttaatta tgccggtact tccaaccctg
      361 ttacgtgaat ttatcgcgtc ggaggacatt gccaatcatt ttggggtcct tttagcctta
      421 tacgcattga tgcaagtaat ttttgcgcca tggttaggca agatgagtga ccgctttggg
      481 cgccgccccg ttcttttgtt atccctgatc ggggcaagcc ttgattacct gttgttggcg
      541 ttcagctctg ccttatggat gttatactta gggcgtctgt tgagcggaat cacgggtgcg
      601 actggtgcgg ttgcagcatc ggtgattgct gatacaactt cagcgagcca gcgtgtgaag
      661 tggttcggtt ggcttggtgc cagtttcggg cttggactga tcgccggccc gatttcggag
      721 gattcgctgg agagatctcc ccacactccc cgttttttat cgcggcactg ctgaatattg
      781 tgacattttt agtggtaatg ttttggttcc gcgagacaaa aaacacgcgt gacaacacag
      841 atacagaggt aggggttgaa actcagtcta attccgtata tattacatta ttcaagacca
      901 tgccaatctt gcttattatt tacttttctg cacaacttat cggccagatc cctgcaactg
      961 tttgggtact gttcaccgaa aatcgctttg gctggaatag catgatggta ggtttcagtc
     1021 ttgcaggttt gggcttactt catagcgtgt ttcaagcttt cgtcgctggg cgtatcgcaa
     1081 ccaagtgggg ggagaaaact gctgtcttat taggttttat tgcagactct tcggctttcg
     1141 cgttcttggc gtttatttca gaaggttggc ttgtgttccc tgttctgatc ttgcttgctg
     1201 gaggcggaat cgcattgcct gctctgcaag gcgttatgtc cattcagacc aagtcacatc
     1261 aacaaggtgc attacaggga ctgttggttt ccttaactaa tgcgaccggg gtcattggac
     1321 ccttgctttt cgctgttatt tacaatcaca gcctgccgat ttgggacggg tggatctgga
     1381 ttattggtct tgctttctac tgtatcatta tcttattatc tatgacgttc atgttgacac
     1441 cccaagccca aggttctaaa caggagacgt cggcttagct cgagcaccac caccaccacc
     1501 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     1561 agcaaaaggc catccgtcag gatggccttc tggtggcact tttcggggaa atgtgcgcgg
     1621 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1681 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     1741 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     1801 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     1861 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     1921 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     1981 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     2041 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     2101 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     2161 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     2221 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     2281 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     2341 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     2401 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     2461 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     2521 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     2581 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2641 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2701 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2761 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2821 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2881 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2941 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3001 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3061 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3121 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3181 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3241 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3301 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3361 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3421 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3481 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     3541 tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag tacaatctgc
     3601 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     3661 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     3721 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     3781 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg
     3841 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta
     3901 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg
     3961 atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga
     4021 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg
     4081 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc
     4141 agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc
     4201 agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac
     4261 ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc
     4321 ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
//
