LOCUS       sequence-219899-        3489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is part of the Minimal Antibiotic Resistance Platform 
            (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that 
            constitutively expresses genes using the Pbla promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Cox G, Sieron A, King AM, De Pascale G, Pawlowski AC, Koteva K, 
            Wright GD
  TITLE     A Common Platform for Antibiotic Dereplication and Adjuvant 
            Discovery.
  JOURNAL   Cell Chem Biol. 2017 Jan 19;24(1):98-109. doi: 
            10.1016/j.chembiol.2016.11.011. Epub 2016 Dec 22.
  PUBMED    28017602
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.11.011"; journalName: "Cell Chem Biol"; 
            date: "2017-01-19- 19"; volume: "24"; issue: "1"; pages: "98-109"
COMMENT     Sequence Label: pGDP1 fosA
FEATURES             Location/Qualifiers
     source          1..3489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             232..254
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             273..290
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             300..317
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             747..764
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(826..853)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(944..1759)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(944..1759)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1667..1686
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1751..1770
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1881..2469
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2370..2389
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2623..2640
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2655..2794
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2720..2739)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2880..2902
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2896..3087)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 agtaaggcaa ccccgccagc ctagccggga gatctggcct attggttaaa aaatgagctg
       61 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
      121 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      181 tgtatccgct catgagacaa taaccctgat aaatgcttct ctagaaataa ttttgtttaa
      241 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc
      301 tggtgccgcg cggcagccat atgctgagcg ggctgaacca tctgacctta gcggtgagcc
      361 agcttgctcc ttctgttgca ttttaccaac aactgttggg catgatgttg catgcccgtt
      421 gggatagtgg ggcgtacctc agctgtggtg atttgtggct gtgcttgtca ctcgatccgc
      481 agcgccgtgt taccccgcca gaagaaagcg attatacgca ctatgcgttc agtattagtg
      541 aggcggactt cgccagtttc gcagcgcgcc tggaagcggc gggcgttgcc gtgtggaagc
      601 tgaaccgttc ggaaggcgcg agccactatt tcctggatcc ggatggccac aaactggaac
      661 ttcatgtcgg cagcctggcc cagcgtttag ccgcttgtcg ggaacagccg tacaaaggca
      721 tggtgttctt tgctgagtag ctcgagcacc accaccacca ccactgagat ccggctgcta
      781 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaaaag gccatccgtc
      841 aggatggcct tctggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      901 tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa aactcatcga
      961 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     1021 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     1081 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     1141 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     1201 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     1261 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
     1321 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     1381 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     1441 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     1501 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     1561 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     1621 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     1681 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag caagacgttt
     1741 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     1801 ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1861 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1921 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1981 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     2041 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2101 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2161 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2221 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2281 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2341 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2401 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2461 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2521 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2581 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2641 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     2701 cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     2761 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     2821 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2881 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
     2941 gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc
     3001 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
     3061 aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat
     3121 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
     3181 acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga
     3241 ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc
     3301 acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga
     3361 cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca
     3421 ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt
     3481 ctgctaacc
//
