LOCUS       sequence-219900-        3670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is part of the Minimal Antibiotic Resistance Platform 
            (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that 
            constitutively expresses genes using the Pbla promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Cox G, Sieron A, King AM, De Pascale G, Pawlowski AC, Koteva K, 
            Wright GD
  TITLE     A Common Platform for Antibiotic Dereplication and Adjuvant 
            Discovery.
  JOURNAL   Cell Chem Biol. 2017 Jan 19;24(1):98-109. doi: 
            10.1016/j.chembiol.2016.11.011. Epub 2016 Dec 22.
  PUBMED    28017602
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.11.011"; journalName: "Cell Chem Biol"; 
            date: "2017-01-19- 19"; volume: "24"; issue: "1"; pages: "98-109"
COMMENT     Sequence Label: pGDP3 arr
FEATURES             Location/Qualifiers
     source          1..3670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             232..254
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             273..290
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             300..317
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             795..812
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(874..901)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        926..1030
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1031..1099,1100..1891)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1249..1268)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2062..2650
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2551..2570
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2804..2821
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2836..2975
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2901..2920)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3061..3083
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3077..3268)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 agtaaggcaa ccccgccagc ctagccggga gatctggcct attggttaaa aaatgagctg
       61 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
      121 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      181 tgtatccgct catgagacaa taaccctgat aaatgcttct ctagaaataa ttttgtttaa
      241 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc
      301 tggtgccgcg cggcagccat atggtaaaag attggattcc catctctcat gataattaca
      361 agcaggtgca aggaccgttc tatcatggaa ccaaagccaa tttggcgatt ggtgacttgc
      421 taaccacagg gttcatctct catttcgagg acggtcgtat tcttaagcac atctactttt
      481 cagccttgat ggagccagca gtttggggag ctgaacttgc tatgtcactg tctggcctcg
      541 agggtcgcgg ctacatatac atagttgagc caacaggacc gttcgaagac gatccgaatc
      601 ttacgaacaa aaaatttccc ggtaatccaa cacagtccta tagaacctgc gaacccttga
      661 gaattgttgg cgttgttgaa gactgggagg ggcatcctgt tgaattaata aggggaatgt
      721 tggattcgtt agaggactta aagcgccgtg gtttacacgt cattgaagac tagaagcttg
      781 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      841 aggaagctga gttggctgct gccaccgctg agcaaaaggc catccgtcag gatggccttc
      901 tggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      961 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1021 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1081 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1141 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1201 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     1261 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     1321 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1381 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1441 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1501 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1561 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     1621 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     1681 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     1741 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     1801 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     1861 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1921 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1981 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2041 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2101 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2161 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     2221 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2281 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2341 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2401 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2461 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2521 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2581 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2641 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2701 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2761 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2821 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     2881 ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     2941 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     3001 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     3061 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc
     3121 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
     3181 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
     3241 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca
     3301 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg
     3361 aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg
     3421 accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc
     3481 cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg
     3541 acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     3601 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
     3661 tctgctaacc
//
