LOCUS       sequence-219901-        5778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is part of the Minimal Antibiotic Resistance Platform 
            (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that 
            constitutively expresses genes using the Pbla promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Cox G, Sieron A, King AM, De Pascale G, Pawlowski AC, Koteva K, 
            Wright GD
  TITLE     A Common Platform for Antibiotic Dereplication and Adjuvant 
            Discovery.
  JOURNAL   Cell Chem Biol. 2017 Jan 19;24(1):98-109. doi: 
            10.1016/j.chembiol.2016.11.011. Epub 2016 Dec 22.
  PUBMED    28017602
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.11.011"; journalName: "Cell Chem Biol"; 
            date: "2017-01-19- 19"; volume: "24"; issue: "1"; pages: "98-109"
COMMENT     Sequence Label: pGDP1 uvrA
FEATURES             Location/Qualifiers
     source          1..5778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             232..254
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             273..290
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             300..317
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             3036..3053
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(3115..3142)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(3233..4048)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3233..4048)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3956..3975
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4040..4059
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4170..4758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4659..4678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4912..4929
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4944..5083
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5009..5028)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5169..5191
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5185..5376)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 agtaaggcaa ccccgccagc ctagccggga gatctggcct attggttaaa aaatgagctg
       61 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
      121 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      181 tgtatccgct catgagacaa taaccctgat aaatgcttct ctagaaataa ttttgtttaa
      241 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc
      301 tggtgccgcg cggcagccat atggctaagg gtttccaccg ccgaaaacag ctcgtgggtg
      361 tcctgtaccc gcaacaaacc ggcacgctga atagccgcat cccatgccgg gtccattcct
      421 gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc cgcttttaat caccagaatc
      481 ggtttattac gcgaggcact acgggccgcc gaaacaaagc gtcgcgcgtc gcttaactgt
      541 tcgagataga gcaggatggc gctggtttta ctgtcgcgtg ccagatagtc aagcaattca
      601 tcaacgtcga tatccaggct gtcgccgagc gcaataaagt aggaaaagcc catcttacgc
      661 tgttgcgccc agtcgaggat ggtgttggag acggcagccg attgcgaaat aaacgccagc
      721 ttgccgcgtt taatcggcac aggcgaaaag ctggcattca gaccttgcca gggagccagt
      781 aaacccagac tgtttggtcc aagcaggcgc atgttatggc gcagggcgca ggcgcggaga
      841 tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg ttttacagcc tttctcgccg
      901 agctcttcca gaagagcaag attacggctg gcattggtac ataaaaccgc aaggtcgggt
      961 gtaaagggca agctggcaat atccggccag gccaacacac ccaacaccgc tttccaggct
     1021 ggcgtcaccg ggagtaccgg tccgttaaag cctcccgcca gcaggttacg catcatcagg
     1081 taacctgcgc gattgggttt catcgacgcg ccaattaccg ctatcgattt tggtcgcagt
     1141 agtgcttcca gtcctcgctg actcataccg gtctcccgca gaagtgaccg aatgatttta
     1201 aacgctttct aactgttctg ctgtgatgct acccagatgt tgcgtttttc ctgccagata
     1261 gcgtgtttta aagcgggtaa aatgctcgcc taaccctgcc gccgccccgg tatcgccggc
     1321 catatctaac agtgcgatgg ctacctcggc agtacaatat tggccttcag cctgggcttc
     1381 acgcaggcga taggcagaaa gccgggaaag atcgacggaa atgacgggaa gattatccag
     1441 atacggactt ttacgaaaca tcttgcgagc ttccggccag gtaccatcga gcatgataaa
     1501 cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc cgttgctcat cagcatacga
     1561 agcgggaaag accaccattg gctgatagtc cgggttttgc accaactcca gcaaatcctg
     1621 cgagggttcg gtacgcgacc attgaaacgc aacggtatca ggcaaaatat cagcaatgag
     1681 acgcccggta ttactgggct tcattggctc ggtgtcgaac atcagcaaac agaagcgact
     1741 ttttgcttgt gctggggtaa ttgtcgaaca gagacataat ttctctggca aaagacagcg
     1801 ttggcagcga cgaacgcgat taccgcgggc aagaaaagga cgtgttgcgc gcgcaatacg
     1861 ctcggcgcgt aactggagaa cagcgttttc ggtcataaga gagcgtcgaa aaaacgccat
     1921 tgtcgcagag gagaaaacgg ggcacaagat gcgccccggt aagattaaag agattcgttc
     1981 agccagctat cgaacggcgc tttcggtact gcgccattaa gcatgtcgac aacctgaccg
     2041 tttttgaaaa tcatgatcgt cggtatacta cgaattccaa agcgactgct caattcacgt
     2101 tcagcttcgg tattcacttt cacaaagcgc actttaccgc tacgctcttg cgcgacatct
     2161 tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg gtgcccagaa gtcgatcacc
     2221 acaggtagat catccttcag caatttgtcg agtgtttcac cggtcgcatt aatcacctct
     2281 ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg catcttcgat ccgatcgtcg
     2341 ggaatgcgat tgatggcctg acaatgggta caaacggtat tcataactaa cctcgggatg
     2401 agtaaggaga cttatcggca acgacgccgg tatgtttcta atatgttaca tattatcggg
     2461 gacattgttc cagacaacaa tctgttttat tcaataggta tgatagtcgc aggctttggt
     2521 acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc gcgcttcgcg cagcgctggt
     2581 ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc aaggtcaaag tgatgtatgt
     2641 ccgcagtgat gatgattctg ataaacgtac ccacaacccg cgtaccggga aagggggcgg
     2701 gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc gcccgcgatg acaaacagag
     2761 tcagccccgt gaccgcaagt gggaagattc gccgtggcgc acggtttccc gcgcgccggg
     2821 tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt ggtaaaagtt ttattgatcc
     2881 ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc tacggcgaaa atgcatgtca
     2941 ggcacttttc cagagccgtc cggaagcgat tgttcgcgcc tggtttatcc agagtgtaac
     3001 gccgcgtttt aaagaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc
     3061 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaaaagg
     3121 ccatccgtca ggatggcctt ctggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3181 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgaattaat tcttagaaaa
     3241 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     3301 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     3361 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     3421 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     3481 gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac
     3541 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     3601 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     3661 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     3721 atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag
     3781 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     3841 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     3901 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
     3961 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctagagc
     4021 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
     4081 acagttttat tgttcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4141 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4201 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4261 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     4321 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4381 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4441 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4501 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4561 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4621 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4681 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4741 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4801 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4861 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4921 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4981 atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5041 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     5101 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     5161 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     5221 gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc
     5281 atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg
     5341 ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt
     5401 tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac
     5461 tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat
     5521 gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt
     5581 aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca
     5641 gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt
     5701 tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg
     5761 tgattcattc tgctaacc
//
