LOCUS       sequence-219905-        3934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is part of the Minimal Antibiotic Resistance Platform 
            (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that 
            constitutively expresses genes using the Pbla promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Cox G, Sieron A, King AM, De Pascale G, Pawlowski AC, Koteva K, 
            Wright GD
  TITLE     A Common Platform for Antibiotic Dereplication and Adjuvant 
            Discovery.
  JOURNAL   Cell Chem Biol. 2017 Jan 19;24(1):98-109. doi: 
            10.1016/j.chembiol.2016.11.011. Epub 2016 Dec 22.
  PUBMED    28017602
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.11.011"; journalName: "Cell Chem Biol"; 
            date: "2017-01-19- 19"; volume: "24"; issue: "1"; pages: "98-109"
COMMENT     Sequence Label: pGDP1 vph
FEATURES             Location/Qualifiers
     source          1..3934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             232..254
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             273..290
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             300..317
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1192..1209
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(1271..1298)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(1389..2204)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1389..2204)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2112..2131
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2196..2215
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2326..2914
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2815..2834
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3068..3085
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3100..3239
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3165..3184)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3325..3347
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3341..3532)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 agtaaggcaa ccccgccagc ctagccggga gatctggcct attggttaaa aaatgagctg
       61 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
      121 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      181 tgtatccgct catgagacaa taaccctgat aaatgcttct ctagaaataa ttttgtttaa
      241 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc
      301 tggtgccgcg cggcagccat atgagaatca ttgagacgca ccgcgatctg ctgagccggc
      361 tcctgcccgg ggacaccgtg ggcggactcg ccgtccacga ggggcagttc caccatgtgg
      421 tgatcggatc gcaccgggtg gtctgcttcg cccgcacccg ggcggccgcc gaccgtctgc
      481 ccggcagggc ggacgtcctg cgcgctcttg ccgggatcga cctcgggttt cgcacgccgc
      541 agccgctgtc cgagggcggc gcccagggca cggacgagcc gccgtacctg gtgctgagcc
      601 gcattcccgg agcaccgttg gaggacgatg tgctcaccag cccggaggtg gcggaggccg
      661 tcgcccgaca gtacgcgacc ctgctgtccg ggctcgcggc ggcgggcgac gaggagaagg
      721 tgcgcgccgc gctgccggag gctcccgcga acgagtggca ggagttcgcc acgggggtgc
      781 gtaccgaact gttcccgctg atgtccgacg gcggccggga gcgtgccgag cgcgagctcg
      841 ccgcgctcga cgccctgccc catctcacct ccgcggtggt ccacggtgac ctcggcggcg
      901 agaacgtcct gtgggagacg gtggacggag tgccgcgcat gagcggcgtc gtcgactggg
      961 acgaggtcgg catcggcgac ccggctgagg acctggccgc catcggggcg agctacggcg
     1021 aggaactgct gggccgagtg ctcgcgctcg gcggctgggc cgacaacgga acggccgagc
     1081 ggatctcggc gatccgaggc accttcgcac tccagcaggc cctctacgcg cagcgcgacg
     1141 gcgacgagga ggaactcgcc gacggcctca gcggctaccg gtaggctcga gcaccaccac
     1201 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     1261 accgctgagc aaaaggccat ccgtcaggat ggccttctgg tggcactttt cggggaaatg
     1321 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1381 attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     1441 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     1501 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     1561 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     1621 gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca
     1681 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     1741 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
     1801 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     1861 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
     1921 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     1981 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     2041 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     2101 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     2161 aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     2221 ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac gtgagttttc
     2281 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2341 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2401 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2461 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2521 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2581 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2641 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2701 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2761 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2821 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2881 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2941 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3001 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3061 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     3121 tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat
     3181 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     3241 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     3301 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     3361 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac
     3421 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct
     3481 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct
     3541 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc
     3601 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac
     3661 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct
     3721 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc
     3781 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac
     3841 cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg
     3901 ttcgctcgcg tatcggtgat tcattctgct aacc
//
