LOCUS       sequence-219916-       10303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral backbone for Gateway cloning of Promoter/Gene insert 
            together with constitutive Zeocin/Bleomycin resistance and Acyclovir
            conditional ablation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10303)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 10303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWX-R4DESTR2-PGK-Tk:Sh
FEATURES             Location/Qualifiers
     source          1..10303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      526..535
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2065..2653
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2118..2138)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2536..2547)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2656..2672)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2657..2673)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(2801..2834)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             2854..3034
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3072..3091)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3137..3192
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3191..3210)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3553..3569)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3553..3569)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3566..3588)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3577..3593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3601..3607,3608..3625,3626..3631))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3646..3667
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3726..3921
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3772..3907
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3927..4061
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3964..3983)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4018..4037
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4129..4146)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4300..4887)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4380..4399)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5058..5849,5850..5918))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5681..5700
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5919..6023)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6054..6433
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             6435..6692
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6739..6864
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7356..7473
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7833..8066
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8251..8295
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    8570..8693
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(8718..8723,8724..8741,8742..8748)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             8802..9461
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8868..8887)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             9803..10108
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9987..10006
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(10163..10287)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtgccac catggcttct
      541 taccctggac accagcatgc ttctgccttt gaccaggctg ccagatccag gggccactcc
      601 aacaggagaa ctgccctaag acccagaaga cagcaggaag ccactgaggt gaggcctgag
      661 cagaagatgc caaccctgct gagggtgtac attgatggac ctcatggcat gggcaagacc
      721 accaccactc aactgctggt ggcactgggc tccagggatg acattgtgta tgtgcctgag
      781 ccaatgacct actggagagt gctaggagcc tctgagacca ttgccaacat ctacaccacc
      841 cagcacaggc tggaccaggg agaaatctct gctggagatg ctgctgtggt gatgacctct
      901 gcccagatca caatgggaat gccctatgct gtgactgatg ctgttctggc tcctcacatt
      961 ggaggagagg ctggctcttc tcatgcccct ccacctgccc tgaccctgat ctttgacaga
     1021 caccccattg cagccctgct gtgctaccca gcagcaaggt acctcatggg ctccatgacc
     1081 ccacaggctg tgctggcttt tgtggccctg atccctccaa ccctccctgg caccaacatt
     1141 gttctgggag cactgcctga agacagacac attgacaggc tggcaaagag gcagagacct
     1201 ggagagagac tggacctggc catgctggct gcaatcagaa gggtgtatgg actgctggca
     1261 aacactgtga gatacctcca gtgtggaggc tcttggagag aggactgggg acagctctct
     1321 ggaacagcag tgccccctca aggagctgag ccccagtcca atgctggtcc aagaccccac
     1381 attggggaca ccctgttcac cctgttcaga gcccctgagc tgctggctcc caatggagac
     1441 ctgtacaatg tgtttgcctg ggctctggat gttctagcca agaggctgag gtccatgcat
     1501 gtgttcatcc tggactatga ccagtcccct gctggatgca gagatgctct gctgcaacta
     1561 acctctggca tggtgcagac ccatgtgacc acccctggca gcatccccac catctgtgac
     1621 ctagccagaa cctttgccag ggagatggga atctctggag ccaatggagt catggccaag
     1681 ttgaccagtg ctgtcccagt gctcacagcc agggatgtgg ctggagctgt tgagttctgg
     1741 actgacaggt tggggttctc cagagatttt gtggaggatg actttgcagg tgtggtcaga
     1801 gatgatgtca ccctgttcat ctcagcagtc caggaccagg tggtgcctga caacaccctg
     1861 gcttgggtgt gggtgagagg actggatgag ctgtatgctg agtggagtga ggtggtctcc
     1921 accaacttca gggatgccag tggccctgcc atgacagaga ttggagagca gccctggggg
     1981 agagagtttg ccctgagaga cccagcaggc aactgtgtgc actttgtggc agaggagcag
     2041 gactgagata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     2101 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     2161 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     2221 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     2281 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     2341 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     2401 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     2461 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     2521 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     2581 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     2641 gccgcctccc cgcatcgata ccgtcgacct cgaggttcgc tttaagacca atgacttaca
     2701 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     2761 actcccaacg aagacaagat gggatcaatt caccatggga ataacttcgt atagcataca
     2821 ttatacgaag ttatgctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     2881 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     2941 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     3001 agaccctttt agtcagtgtg gaaaatctct agcagcatct agttgaatcg ccagtgtgtc
     3061 gagctagcag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     3121 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     3181 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     3241 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     3301 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     3361 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     3421 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     3481 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
     3541 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     3601 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     3661 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     3721 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     3781 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3841 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     3901 ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat
     3961 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4021 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg
     4081 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4141 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4201 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4261 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4321 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4381 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4441 gcttacggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4501 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4561 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4621 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4681 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4741 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4801 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4861 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4921 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4981 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5041 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5101 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5161 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5221 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5281 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5341 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5401 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5461 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5521 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5581 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5641 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5701 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5761 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5821 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5881 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5941 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6001 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg
     6061 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     6121 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     6181 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     6241 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     6301 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     6361 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     6421 cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     6481 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     6541 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6601 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6661 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     6721 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     6781 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     6841 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     6901 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     6961 agcagggagc tagaacgatt cgcagttaat actggcctgt tagaaacatc agaaggctgt
     7021 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     7081 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     7141 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa agcacagcaa
     7201 gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg agtagtagaa
     7261 tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc tgaacatctt
     7321 aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     7381 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     7441 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagaaat
     7501 ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt agtaatacaa
     7561 gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag ggattatgga
     7621 aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta gaacatggaa
     7681 aagtttagta aaacaccata aggaggagat atgagggaca attggagaag tgaattatat
     7741 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     7801 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     7861 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     7921 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     7981 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     8041 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     8101 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     8161 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     8221 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     8281 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     8341 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     8401 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     8461 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     8521 tccattcgat tagtgaacgg atctcgacgg tatctagtga ttatcggatc aactttgtat
     8581 agaaaagttg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     8641 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg
     8701 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
     8761 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     8821 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     8881 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     8941 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     9001 tgaatgctca tccggaactc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     9061 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     9121 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     9181 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     9241 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     9301 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     9361 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     9421 aattacaaca gtactgcgat gagtggcagg gcggggcgta aacgcgtgga tccggcttac
     9481 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     9541 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     9601 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     9661 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     9721 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     9781 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     9841 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     9901 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     9961 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
    10021 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
    10081 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
    10141 aggtcgagca gatctgctcg accatagtga ctggatatgt tgtgttttac agtattatgt
    10201 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
    10261 tcgttcagct ttcttgtaca aagtggtgat aatcgaattc cac
//
