LOCUS       sequence-219922-        7930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of OCTN2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Czeredys M, Samluk L, Michalec K, Tulodziecka K, Skowronek K, Nalecz
            KA
  TITLE     Caveolin-1--a novel interacting partner of organic cation/carnitine 
            transporter (Octn2): effect of protein kinase C on this interaction 
            in rat astrocytes.
  JOURNAL   PLoS One. 2013 Dec 13;8(12):e82105. doi: 
            10.1371/journal.pone.0082105. eCollection 2013.
  PUBMED    24349196
REFERENCE   2  (bases 1 to 7930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-13"; volume: "8(12):e82105. doi"; pages: " 
            10.1371/journal.pone.0082105. eCollection 2013"
COMMENT     Sequence Label: p3 FLAG_CMV14/OCTN2 (wt)
FEATURES             Location/Qualifiers
     source          1..7930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2296..2361
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    2372..2994
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2571..2590)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3018..3038)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3036..3333
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3387..4181
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3441..3460)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4051..4070
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4833..4967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4870..4889)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4924..4943
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4972..4991)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4973..4991)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5005..5021)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5005..5021)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5018..5040)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5029..5045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5053..5059,5060..5077,5078..5083))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5098..5119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5236..5253)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5407..5995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5487..5506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6166..6957,6958..7026))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6789..6808
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7027..7131)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7158..7613
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7245..7264)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7455..7476
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7739..7761
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7753..7770
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7754..7770
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattaagc ttatgcggga ctacgacgag gtgaccgcct tcctgggcga gtgggggccc
      661 ttccagcgcc tcattttctt cctgctcagc gccagcatca tccccaatgg cttcaatggt
      721 atgtctatcg tgttcctggc ggggaccccg gagcaccgtt gccttgtgcc tcacaccgtg
      781 aacttgagca gcgcgtggcg caaccacagt atcccgttgg agacgaagga cggacgacag
      841 gtgcctcaga gctgccgccg ctaccgactg gccaccatcg ccaacttctc tgcgctgggg
      901 ctggagccgg gacgggacgt ggacctggag cagctggagc aagagaactg cctggatggc
      961 tgggagtaca acaaggacgt cttcctgtcc accatcgtga cagagtggga cctggtgtgt
     1021 aaggatgact ggaaagcccc actcaccacc tccttgtttt tcgtgggtgt gctgatgggc
     1081 tccttcattt cgggacagct ctcagacagg tttggtcgca agaatgtgct gtttttgacc
     1141 atgggcatgc agactggctt cagcttcctg cagctcttct ctgtgaactt cgagatgttt
     1201 acagtgcttt ttgtccttgt tggcatgggt cagatctcca actacgtggc agcatttgtc
     1261 ctgggaacag aaattctttc caagtcaatt cgaattatat tcgccacctt aggagtttgc
     1321 atattttatg catttggctt catggtgctg cctctgtttg catacttcat cagagactgg
     1381 aggatgctgc tgcttgcgct cactgtacca ggggtgctgt gtggggctct ctggtggttc
     1441 atccctgagt ccccccgatg gctcatctct caaggccgag ttaaagaggc agaggtgatc
     1501 atccgcaaag ctgccaaatt caatgggata gttgcgcctt ccactatctt cgatcccagt
     1561 gagttacaag acttaaattc caagaagcct cagtcgcacc acatttatga tctggtccga
     1621 acacgaaata tcaggatcat caccatcatg tccataatcc tgtggctgac catatcagtg
     1681 ggctacttcg gactttctct tgacactcct aacttgcatg gggacatcta tgtgaactgc
     1741 ttcctactgg cagctgttga agtccccgcc tatgtgttgg cctggctgtt gctacagcac
     1801 ctgccccggc ggtattctat ctctgctgcc ctcttcctgg gtggcagtgt ccttctcttc
     1861 atacagctgg tgccttcaga attgttttac ttgtccactg ccctggtgat ggtgggcaag
     1921 tttggaatca cctctgccta ttccatggtc tatgtgtaca cggccgagct gtaccccact
     1981 gtggtcagaa acatgggtgt gggggtcagc tccacagcat cccgccttgg cagcatcctg
     2041 tctccctact ttgtctacct cggtgcctat gatcgcttcc tgccttatat cctcatggga
     2101 agtctgacca tcctgacagc tatcctcacc ttgttcttcc cagagagctt tggtgcccct
     2161 ctccccgaca ccattgacca gatgctcagg gtcaaaggaa taaaacaatg gcaaatccaa
     2221 agccaaacaa ggacgcaaaa agatggtgga gaaagcccaa cggtcctaaa gagcacagcc
     2281 ttcggatccc gggctgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     2341 tacaaggatg acgatgacaa gtagtgatcc cgggtggcat ccctgtgacc cctccccagt
     2401 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     2461 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     2521 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     2581 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     2641 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     2701 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     2761 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     2821 gtgaaccact gctcccttcc ctgtccttct gattttaaaa taactatacc agcaggagga
     2881 cgtccagaca cagcataggc tacctggcca tgcccaaccg gtgggacatt tgagttgctt
     2941 gcttggcact gtcctctcat gcgttgggtc cactcagtag atgcctgttg aattgggtac
     3001 gcggccagct tggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3061 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3121 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3181 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3241 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3301 gctattccag aagtagtgag gaggcttttt tggaggaatt gatcagcttg ggatctgatc
     3361 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     3421 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     3481 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     3541 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     3601 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     3661 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     3721 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     3781 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     3841 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     3901 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     3961 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     4021 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     4081 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     4141 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     4201 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     4261 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     4321 cggggatctc atgctggagt tcttcgccca ccccgggctc gatcccctcg cgagttggtt
     4381 cagctgctgc ctgaggctgg acgacctcgc ggagttctac cggcagtgca aatccgtcgg
     4441 catccaggaa accagcagcg gctatccgcg catccatgcc cccgaactgc aggagtgggg
     4501 aggcacgatg gccgctttgg tcgacccgga cgggacgctc ctgcgcctga tacagaacga
     4561 attgcttgca ggcatctcat gagtgtgtct tcccgttttc cgcctgaggt cactgcgtgg
     4621 atggagcgct ggcgcctgct gcgcgacggc gagctgctca ccacccactc gccaagctgg
     4681 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgccgatc ataatcagcc
     4741 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     4801 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     4861 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4921 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaat tccctatagt
     4981 gagtcgtatt aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     5041 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5101 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5161 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5221 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5281 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5341 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5401 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5461 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5521 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5581 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5641 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5701 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5761 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5821 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5881 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5941 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6001 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6061 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6121 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6181 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6241 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6301 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6361 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6421 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6481 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6541 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6601 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6661 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6721 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     6781 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     6841 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     6901 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     6961 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7021 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7081 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7141 ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7201 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7261 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7321 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     7381 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     7441 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     7501 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7561 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttccattcg
     7621 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     7681 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     7741 cagtcacgac gttgtaaaac gacggccagt gccaagctga tctatacatt gaatcaatat
     7801 tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca
     7861 ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga
     7921 ccgccatgtt
//
