LOCUS       sequence-219923-       10848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to 
            myotubularin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10848)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 10848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-MTM1
FEATURES             Location/Qualifiers
     source          1..10848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2672..2692)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2700..2716)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2732..2866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2756..2775)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2810..2829
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(3305..3492,3493..3504)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3538..3554)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3538..3554)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3591..3611)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3591..3609)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3630..3646)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3643..3665)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3654..3670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3678..3684,3685..3702,3703..3708))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3723..3744
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3861..3878)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4032..4620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4112..4131)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4791..5582,5583..5651))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5414..5433
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5652..5756)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5919..5940)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(6289..6300,6301..6463)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6836..6852
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6872..6892
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggctactttt cgacgagggt ctggcttcca
      781 tttacgcctg cttagagaaa actccaggct aaatgcggca cttgagagct gtcgtaaagt
      841 aaacgcagtg atggcctcgg ctcctatagc accttataac tccagtcctc ttgggaacag
      901 ctccagccaa aacgcgtcca gagagagcct gaagatggag ccgctggcag atgttacgct
      961 gctgccggga gaagagagag tgatcgataa agatatcatc tacatctgcc cctataatgc
     1021 tgcagtcaaa ggaaaggtgt tcatcaccaa ctacagactc tacttcaaaa acactgactc
     1081 agagacacca gttaccctga acatcccctt aggtgtcata agccgtgtag aaaagatggg
     1141 cggagcctcc agtcgaggag aaaactctta cgggcttgaa atcacctgca aggacatgag
     1201 gaacctgcga tttgctctga agcaagaagg ccacagtcga agggacatat ttgagattat
     1261 tttcaaatac gctttcccac tttctcacgg aatgcaactc tttgcctttt taagtcagga
     1321 gaagtatgag gagaatggct ggaacgtgta tgaggccttg gaagagtata ggaggcaggg
     1381 tctacccaat aatgagtgga gggtcacttt catcaataag aactacgagc tctgtgacac
     1441 ctatcccaca attcttgttg ttccctacaa ggccacagaa gatgatctac gaaaagtggc
     1501 tacatttcga tcgcgctgcc gcattccggt gatgtcgtgg ttccataagg agaaccatgc
     1561 tgtgatcacg cggtgtagtc agcctttagt gggtatgagt ggcaagcgca acaaagatga
     1621 tgagcgttat ttggacctaa tccgggaggc caatggtact accaaactca ccatctatga
     1681 cgccaggccc aacgtcaatg ctgtggcaaa caaggccacg ggcggaggtt atgagggtga
     1741 tgatgcttat cagaacgcag aactggtctt tctggacatt cataatattc atgtcatgag
     1801 ggagtcactg aagaagctaa aggacattgt ttaccccaat gtggaggaat ctcattggct
     1861 ctccagcctg gagtccacgc attggctaga gcatatcaag ctggtgctgt cgggggccat
     1921 tcaggtggct gataaggtgt cttctgggag ctctgtgatg gttcactgca gtgatggctg
     1981 ggatcgcact gctcagctca catctctgtc catgctgatg ctggactcct actataggac
     2041 tctcaggggc ttccaggtct tgatggagaa ggagtggatc agcttcggac acaagtttgc
     2101 ctcaagaatt ggccatgggg acaaaaacca tgcggatcag gacaggtcac ctatcttttt
     2161 gcaatttatt gactgcgtgt ggcaaatgac taaacagttc ccaacagcgt ttgagtttaa
     2221 cgaacgtctc ctggtggtca ttctggatca tctgtacagc tgccgcttcg gcactttcct
     2281 ctacaactgt gagagtgttc gtgacagcaa taatgttcgg acaaagactg tgtctctgtg
     2341 gtcttacatt aatagtgaca cgtctttgta tataaacccc ttctacacgg cggagtccag
     2401 tcgggtgctg taccctgtag ccagcatgcg ccacctagag ctgtgggtca cttactacat
     2461 caggtggaac ccgcgcatac aacaccagca acagagtcca gtggagcagc gctacaaaga
     2521 gcttctggca ttaagggatc agtacctgaa gaaactagag gagctgcagc tctcagactc
     2581 cgccccttcc tccacccact tgacaaacag ctccaccccc aatgctgcca cgcccaatca
     2641 gcacatcaca catttgcaga caccattttg acaactttat tatacatagt tgataattca
     2701 ctggccgtcg ttttacggta ccatcgatga tgatccagac atgataagat acattgatga
     2761 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2821 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2881 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2941 cctctacaaa tgtggtatgg ctgattatga tcctctagat cagatctgcg aagatacggc
     3001 cacgggtgct cttgatcctg tggctgattt tggactgtgc tgctcgcagc tgctgatgaa
     3061 tcacatactt cctccatttt cttccactga ttgactgtta taatttccct aatttccagg
     3121 tcaaggtgct gtgcattgtg gtaatagatg tgacatgacg tcacttccaa aggaccaatg
     3181 aacatgtctg accaatttca tataatgtga aaacgatttt cataggcaga ataaataaca
     3241 tttaaattaa actgggcatc agcgcaattc aattggtttg gtaatagcaa gggaaaatag
     3301 aatgaagtga tctccaaaaa ataagtactt tttgactgta aataaaattg taaggagtaa
     3361 aaagtacttt tttttctaaa aaaatgtaat taagtaaaag taaaagtatt gatttttaat
     3421 tgtactcaag taaagtaaaa atccccaaaa ataatactta agtacagtaa tcaagtaaaa
     3481 ttactcaagt actttacacc tctggttctt gaccccctac cttcagcaag cccagcagat
     3541 ccactagttc tagagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga
     3601 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     3661 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     3721 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     3781 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3841 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3901 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3961 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4021 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4081 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4141 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4201 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4261 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4321 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4381 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4441 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     4501 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4561 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4621 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4681 ggcattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4741 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4801 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4861 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4921 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4981 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5041 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5101 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5161 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5221 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5281 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5341 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5401 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5461 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5521 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5581 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5641 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5701 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5761 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     5821 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     5881 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     5941 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     6001 cacgctggct aagaactcat cagcctcccc ggtccatcta cccacgtacc aatgcaccaa
     6061 ttggccacaa tgacggctac tacatggtgc cattccttcc tctttatagg aatggagact
     6121 acctcctgtc caacaatgct cttggatacg agtacgccta cctgttggac ccaggtcatt
     6181 gcacaacacc agaaatgccc tctgatctgc aaaagacgtg aatatctgtt cagacaccca
     6241 tatccactct gttccacaca ggtcagaggt ttgtccagga gttcttgaca gaggtgtaaa
     6301 aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat tttactcaat
     6361 taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag tactccatta
     6421 aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac tagagattct
     6481 tgtttaagct tttaatctca aaaaacatta aatgaaatgc atacaaggtt ttatcctgct
     6541 ttagaactgt ttgtatttaa ttatcaaact ataagacaga caatctaatg ccagtacacg
     6601 ctactcaaag ttgtaaaacc tcagatttaa cttcagtaga agctgattct caaaattgtt
     6661 agtgtcaagc ctagctcttt tggggctgaa aagcaatcct gcagtgctga aaagcctctc
     6721 acaggcagcc gatgcgggaa gaggtgtatt agtcttgata gagaggctgc aaatagcagg
     6781 aaacgtgagc agagactccc tggtgtctga aacacaggcc agatgggccc tcgagcagga
     6841 aacagctatg accatgatta cgccaagcta tcaactttgt atagaaaagt tggcacgcgc
     6901 agattccgtg tgggcctggt aatatcttac cattttaagc tattttaaga ttagggaaaa
     6961 aaattaaata aaactacttt aaaattactt tccttaaaaa gcaacatatt tatttagttt
     7021 ttgtttaact ggctcaagat tgtaatattt tacttttaca tgttttttta agtacctaaa
     7081 tgtgttcaaa agtaaagtat gttattgttt ttttttttta atgttagcta agctttaaat
     7141 tgtaatatat tacttttaaa agtgactttt taaaaatgta aataaaaaag taactataaa
     7201 tgtgtaatgc atgttatttc tgtgggtttt taattttcat ctcaactggg cttacaattt
     7261 taacataata aaaatcaaat caaatcaaat caggccctta tttgtcacat acactcttgt
     7321 atactgaaat aaactaaatg ttgagaaaaa tttaaataca ccaaaataac gcataccttt
     7381 tttgtaatta cctaagtaaa acatttagtt ccttttttaa gtgactcaag attgtaatct
     7441 attaattgta aaagataaaa ctagtgatca atctgacgtg cttctatgca gcctgtgaga
     7501 ttaattgtga tgtttaatta ttaaacaatt taaatggatc aaacatttca catgtgctta
     7561 ttgtgtccaa caaaaccagc agaagcactt aaaaatattg catgacaaca tgagttttta
     7621 atgtaaatta tatatacttc ctaaacgaag ttaatcatat ttctaaaata ataatgtcat
     7681 tgaaatattt aaataaatga taagctttag ttgattcaac ataaaattga aagcaataag
     7741 gacttttttt ttttacagcg gtgaagcaaa agcatgtaat aatcatataa tataataaat
     7801 aaataaataa ataatggctc ttggtgcaaa tatgaacact ttaaataaat atagaataaa
     7861 tatgatcaat aagtcataac gatgtgtttt atgttgatta taatttaaac atgtttctaa
     7921 cgcaagtttt aaaaactgtt ttaaagtatt attttaagtt ataagatcaa tttgactcaa
     7981 cattttgtag tgaaaatatg ttgtgtaaaa ttaatataat atacactata aaaatgcagg
     8041 gttccacaca atacattaat gttgtcccaa cacaaatcga ttaagttaac aaatatatgt
     8101 ggattgaaca taaaaaatta agttgtccca atgaaatctc aagaattgtg ttgtttcagc
     8161 tcattttaaa taagtagttt taacaagtaa aaaataacat atttttgagt gtgtatatat
     8221 atatattaca aaatataata atataaaatg taaaagatat aaaccatgct acatatattt
     8281 aagagctatt ttgcacatta cttttttgca agccatttta gtgttttttt tacaaatata
     8341 tattagctaa atatacaata tcttaaatat acaattaatc agacatgcaa ttaatgcatt
     8401 taacctacat aaaaatacat gatcaacata tgttttatgc tgattacaac taaatcaaac
     8461 gtaatgaaat taatttataa aagcgattta aatatgtcaa tattttaagt taaaatcact
     8521 tgaaacctac tataaatgaa cttaaattaa aaaaatctcc tttaattcca atgcaacagt
     8581 gttgtttttt cagctgagga cccccggtgc agatggttgg cccccggccc gctattctaa
     8641 ggaagccttt cttaatttta gggcggcgct gcatgcgctc agatcagatt cacgggtgga
     8701 gagggttatt ttctcttttt tattttttct tgcctgctca tatttgggcc tgtgtgaacc
     8761 ccaggctccg cctcggcccc caggagccgc tcttatttaa aggggatcct cgccttcagt
     8821 cttcgtccca gtggaagcat cagtcttgtg ataacaactt ggtgagtcct acagaccagc
     8881 gctcgactct aggctttaaa tgcattgatt tctctggatt agctaccggg agagttttaa
     8941 ctttgcttat tagtcttgtg ctcgaactgc taggtttaaa gagtgttttc tctaatgcat
     9001 gcatgaagcg acagctgaaa accagaaata gagcgtttgt cctcctgtta aactctagcc
     9061 tttctttggc atttgtcagt ccgagtcttc agctgccggc aggagattgg gtttcagtaa
     9121 cacttgctga gcacaaacta cacgtgcttt cttcagattt aattcattat taatcattct
     9181 agatttaaat gatgttctta ctcattgctg attattttag ccttgcggag cagattaaat
     9241 gagcacgtgt ttcattaaga ttgatttaaa tataattaat atttcaccac tgtagtttta
     9301 aaattgcctt ctaagtcatg gttgattttt aataggtatc ggctctaatt agattactat
     9361 tttggttttt aaatgttatt tctatttcat ttgtaggctc gtttttaatg tgattttatt
     9421 agattctagt tttaaaatca tctcttagcc ttaatgagca gactgaatga acatgtttca
     9481 ttaagattaa cttcattata atgaattatt taatcattct agtgggttat aaatcagctc
     9541 tatttaaatt aattgtcgtg tttatttcta tttaattttt gattattttt tcagggtcgt
     9601 ttttatgtga ttttttcccg tttctaattt taaactaatt gtttgatttt tatatgattc
     9661 gactcttttt aaatgaattg ttttgctttt tttattgagc atatatatat tatacaattt
     9721 caatcaacaa ttgttgtgct ttttaaatgt tgtttctatt tcatttctag ggtcgatttt
     9781 attcgattct agttttaaaa tcgtgatttt taacttctaa ccttactgag cagactgaat
     9841 gaacatgttt catcaagatt aacttcatta tagttaatta ttgaatcgtg ctagtggttt
     9901 ataaatcagc tctatttaaa ttaatcatca tcattatttc tatttaattt ttattatttc
     9961 tttaggatta tttttatgtg atttttttca cgttctagtt ttaaactcaa tgctcattac
    10021 tgctcaccct tgctgagcag tctttcgtta agattaaatt aattaataac cattctagtt
    10081 ttaaaacagt gtcttaaggc atagctgata ttttacaagt ttactattta atatatctcc
    10141 tttctttttt tagggtcgat ttcatctgat ttgattaatt tttattatct tcactaagct
    10201 ttttgttcta ggttttattc tcagtattat tactgttata tttgaactga atttctcctc
    10261 atagtttatt cattttattt atttattttt tgtttattcg ctattttgta ttttagtttt
    10321 taattagaaa ttttattaga atcgttttga actagttttt atgcactcta acattaatat
    10381 taaatcataa ctattatttt tattataatt tatttgtgtt tatctttttg tgtagctttc
    10441 atgtgttttt attttagttt tattatgttt gcattgaatt ttttggacta gtttatataa
    10501 cgatatacag aaaagccatg acaacaaatg tttttataat acctattaag tataagctaa
    10561 tcagatttcc actattctgt tcaaagttaa cattattagc cattctgatg gatcatttga
    10621 catttgtcat atgtctttat gaacactgct ctttcagatg ccacttttaa agctgttttt
    10681 tacagtaaat gaacgtttat tattatgaca ttatttccta aggcctctag agtacagcag
    10741 gatgtggcgt gtgcattacc gtgtaattat gctgactgta aatgtgcatt tatagactgt
    10801 cagcatgtct gactgtgcgc tgttgtgatt ggtcctgcac agaccaag
//
