LOCUS       sequence-219924-       15195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to dysferlin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15195)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 15195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-DysF
FEATURES             Location/Qualifiers
     source          1..15195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1448..1459
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7019..7039)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(7047..7063)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7079..7213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7103..7122)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7157..7176
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(7652..7839,7840..7851)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7885..7901)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7885..7901)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7938..7958)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7938..7956)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7977..7993)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7990..8012)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8001..8017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8025..8031,8032..8049,8050..8055))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8070..8091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8208..8225)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8379..8967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8459..8478)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9138..9929,9930..9998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9761..9780
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9999..10103)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10266..10287)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(10636..10647,10648..10810)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     11183..11199
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11219..11239
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtgttgagg tgtttgctac agagagcgac
      781 caacctgaga aatgtagaga agggggaaag gcatagcgac ccgcttgcta gcatcacttt
      841 cagagggaca aaaaagaaga ctaaagtcat caaaaataat gtcaatcctg tctggaatga
      901 gggctttgag tgggacctga agggtgttcc tttagattcg ggagcagagc ttcatgttgt
      961 cgttaaagac catgagaaaa tgggcaggaa taggtttctg ggggagtgtc gagttgctct
     1021 tcgagatgtg ctcaattctc caaacctggc tgctactttt accgtgtccc tggtcgacac
     1081 aaagagaaac agcactgggg ccacaataac cctgcaggtc tcctacatcc ctcctccagg
     1141 gatgacgccc atctttcagc ctcctccaca gccagaggcc cagcacacac cggtggagct
     1201 ggacacagtc acagttttct cattggacac tatgggcgag gatgacactg agagcatgct
     1261 gatgatggag gtggcggagg agcctgaacc tgttccagga ccccaggggc aggacatcgg
     1321 cgcccctaca gcaccaccca aaaaggctcc acccaacttt aaccagggac taaagaaaga
     1381 gaaacgccaa agcaacaaat cccccctgtc cgacaaagcc caggacctcc aggttcggct
     1441 gcgcatcatt gaaggaaggc agcttcctgg tgtcaacatc aaaccagtcg tcaaagtcac
     1501 agttggagga cagacgaaga gaacccgcat taaaaagggc aataatccat tttttgatga
     1561 gacctttttt ctaaacttct ttgagaaacc ttcagatctt tttgatgagc caatattcat
     1621 aaccgtattt gattccaagt cactacggaa agactctgtc attggagaat tcgagctgga
     1681 tgttggcatg gtttataatg aacaccgaca tgctattctc agaaaatggt tgttgctggc
     1741 agaccctgat gatctttctg caggggccag aggttatttg aaggtcagca tgtttgtgct
     1801 ggccgcagga gatgaacctc cgatggataa gaaagagggt gtggaggaaa aggaggacat
     1861 agaggccaat ctgttgagac cagctggtct aaccctgaga ggagccacgt tcactctgag
     1921 agtctacaga gcagaagacc ttcctcagat gggtgatgct cttattgacg atgtcaaaca
     1981 tttccttggc tttgacacta atcgaaagaa cctggtggat ccattggtgg aggtcaactt
     2041 tgcaggaaaa acggtaagta gtaagattct ggagaaatgc gctaaccctc agtggaacca
     2101 gtgcctcact ctgcccatca ggtttccatc catgtgtgaa aagatgagaa tacgtatttt
     2161 agattgggat cgtgcgagtc ataatgatgt cattggcact gcacacttat gcatgtctaa
     2221 aatctcagcc cctggaggag atattgaagt ggatgacagt ataggctttc ttccgacctt
     2281 tggaccctgc tttgtcaaca tgtatgggag tcctcgagag ttcacagctt tctcagaccc
     2341 acacgaggca ctaaacctgg gaaagggaga gggtgtggcc tatcggggac gtgtgttgtt
     2401 ggagctcata acaaagcttt ctgatcaggt ggaccaaagg tctgaagaca tcaactctga
     2461 tgacctgcta gtggtagaaa aattcatgcg gaagaggaag ttttccctgt ttgctgcgtt
     2521 ctactcggcc actctccttc aagatgtgga cgatgcagtt cagtttgaag tcagtatcgg
     2581 taactacggc aacaagttcg acaacacctg tttgcctctg gcatccacca ctcaattcag
     2641 cagagctgtg ttcgacggct gccattacta ttacctgcca tggggaaatg tgaaacctgt
     2701 ggtggtggtg tcttcagagt gggaagacat tggccctcgt atagaggccc tcaacatgct
     2761 gcttagcata gtcgaccgac tggagtcaaa cttggaacag gtacagctgg agctcaaagc
     2821 aagaagtgac ggtcaggaag tcgagcagag ggttgtccag ctgcttgatg atgtcattgc
     2881 agactgcagt gaggaactcc ctgagttgga caaatggcct tgtgtgactc cactggataa
     2941 agctctgagg aatctgcgca agattaacat gcagcagata gtgcaggcgg ccctggcact
     3001 caaacatgga ccagagactg aactgagtgc agtgctggag caggctgagg actggaccat
     3061 gagactacgc tacctctctg aggagcccca aaacagtctg ccagatataa taatctggat
     3121 gcttcaggga gacagacgag tggcctacca cagaatccct gcccatcaag ttctcttttc
     3181 ctatggttac tgtggcaagc actgtggcca acttcagacg gttttcatga agtgtccaca
     3241 ggaagatggg ggatgtagta aaatcccagc tcagctcaga atcaaggtgt ggttcggact
     3301 gactgcggat gtcaaatctt ttaaccagtt tgcagagggc aaactctcag tgtttgcaga
     3361 aacgtatgag aaccagacta aactggctct ggtggggaac tggggtacga cgggtctgac
     3421 ttaccccaag ttcagtgatg tgaccggcag agtcaaactg ccaaaggaga gcttcaaacc
     3481 ctcttcaggc tggagctggg caggagaatg gttcatcagc ccagagaaaa cgatgctgta
     3541 tgatgctgat gcaggtcata taagcttttt ggaggaggtg tttgaaaatc agttgcggtt
     3601 gccaggagga cagtggattg gcatgccgga ggggtacaca gatgtgaatg gagaaaaggc
     3661 tgttcctaaa gatgagatcg agtgtccccc tggctgggtg tgggaggata ttgagtggag
     3721 cgaggatctg aagcgtgcag tggatgatca agggtgggaa tatggaataa ccctcccccc
     3781 ggaccgccga ccaaagtcct gggttccatc tgagaagatg taccatacca accgtcggag
     3841 acgctggatc agactccgcc gtagagacat gcagaaaatg gaggcgctca gaaaacagcg
     3901 gccagacgag tccgagcgtg agggctggga atatgcctct ttatttggct ggaagtttca
     3961 cctcaagcag aagaaaaccg acttgttccg tcgccggcgc tggagacgtc gaatggagcc
     4021 attagaaaag acagggccgg cggccatctt tgccttggag tgttcgctga gcagctttac
     4081 tgatgaaaag aatgacgata agtcagtcac tacaacgttt ggtgtcagcc ggccgactat
     4141 ctcctgcttc tttgatagtg ggaccctgta tcatttgcgc tgttacttgt atcaggccag
     4201 agacctgctg gctatggaca aggacagttt ctcggatgcc tatgccgttg tatcgttcct
     4261 gcatcagagt cagaagacgg tgactgttcg aaacacactc aaccccacct gggaccagac
     4321 cctcattttc tacgaagtgg aaatttttgg agaccatctt gtgactgaga ggagtcctcc
     4381 aaacattgtg gtggagctgt acgatcagga cacctatgga gctgatgagt tcatgggccg
     4441 ctgtgtttgc caaccatcaa tggcatcttc ccctcgcctc gcctggtatc ccatccagaa
     4501 agcagggaat tctgcaggag atctgcttgc tgcttttgaa ctgatccgca gagataaacc
     4561 tgcagttcat cacatcccgg gtcaagaggg agatattata ccaccctcac atatcatgga
     4621 tgagcttgtg cttccagggt tcctctctga gaatcaacag caatggccag aggactctga
     4681 tttgccctat ccaccacctc agagggagcc aaacgtctac atggtgccac aggggataaa
     4741 acctgtccta cagcgcactg ctattgagat actggcatgg ggtttgcgta acctgaagag
     4801 tttccagctg gccagtgtca cgtctcccag tcttcaggtg gaatgtggcg gatctgtggt
     4861 tcagtcgtgc gtcattcgaa atattaagaa gaaccctaat tttgacgtca acatcctgtc
     4921 cattgatgtc cgattgccta aggaagagct ctacatgccc ccattggtga tcaaggttat
     4981 agacaaccgg cagtttggca ggaagcctgt ggtgggccag tgttcagtcc actcgctcaa
     5041 tgagttctac ttccagccgg aaagtctgga gagagactca acagaagagg aatttgaagt
     5101 tattgctcac acacctagag atgatgttct ggttgatatt gatgacaagg agcctctaat
     5161 ccctggacag caggatgaat ttatggactg gtggagcaag ttttatgcct ccatgggtga
     5221 aaggaccaag tgtgggagtt atctggagaa agggttcgat atgctgcagg tgtatgacag
     5281 agaactggag aagactgaag gctttgagca atttgctgat ttctgtcaaa cgtttaaact
     5341 ttaccgtgga agaacccaga atgacagcga agatccttcc gttgttggag agttcaaggg
     5401 ttcgtttaag atctatccat tgcctgatga tccccctatg cctgtaccca cacgtcagtt
     5461 tcgcaaactc cccccgaacg gtatagagga gtgcttggta cgcatctaca tcatccaggc
     5521 ttttgggtta cagcccaaag atgccaatgg caagtgtgac ccctacgtta agatcacgct
     5581 tggcaagaaa tcagtcgatg accacgacaa ttacataccc tgtactttgg agcccgtctt
     5641 tggaaagatg tttgagctga cctgctgtct gcctcttgag aaagacctga agattacact
     5701 ctatgattac gacatggtga ctaaagatga gaaaatcgga gagaccatca ttgatttgga
     5761 gaaccgcttc ttgtctcgtc atggtgctcg ctgcggcctt ccgcagtcct actgtctgtc
     5821 aggaattaac cagtggcgag atcaacttaa accatctcag ctacttcatc gtctgtgtga
     5881 gaggcgaaac tacaaactcc ccgtctacaa gcaggatcga atttacttca gaggacatga
     5941 atacactgtc gcagaccttg atgaagggaa acctcttaac cctcacttgg gaccagtgat
     6001 ggagcgtctg gctctgttgg tgttgaggag gcatggtttg gtgcctgaac atgtcgagac
     6061 gcgacccctc tacagccccc ttcagccaga tatagaacag gggcgactac aaatgtgggt
     6121 ggacctgttc cctaagtctt taggccctcc aggtcctcca ttcaacatca ccccacgcaa
     6181 agctaaaaag ttcttcctgc gctgtattat atggaacaca agagatgtaa tcttggatga
     6241 tgtcagcgtt accggggaga agatgagtga catttatgtg aaaggctgga tgcacggcca
     6301 tgaggagaac aagcagaaaa cagacgtgca ctaccgatcg ctgggtggag agggtaactt
     6361 taactggcgc ttcctcttcc ccttccacta tttacctgcg gaacaactgt gcaccattga
     6421 taaaaaggag cacttttgga gtctggataa agttgaaacg aagcttcctc ctaaacttac
     6481 tatccagatt tgggacaatg acaagttctc ctttgatgat ttcctcggct ctttggagat
     6541 ggatctgaac cacatgctga gtcctgcgaa gtctccagag aagtgtggtc ttgagatgct
     6601 cactcagtcc caggacaaac tggtctcttt gcttgagcag aaaacggtga aaggctggtg
     6661 gccgtgtgtc tgtgacaaag aagacaagaa gatacttgct ggaaaggtgg agatgtctct
     6721 ggaaatagtc tgtgaacagg agcaggatga gaggcctgca ggactcggcc gagatgagcc
     6781 caacatgaat ccccatctag aggagcctca gcgtcccgag acatcattct tatggttctc
     6841 gtccccatat aagacgttta ggttcattgt gtggaggagg tttaagtggc ttattctggg
     6901 cctctttata ctccttttca ttattctctt ccttggagta tttctctacg ccttccctaa
     6961 ctatgctgct atgaagatgg ttggaccctt cagtggacta ggaaagggaa cacagtgaca
     7021 actttattat acatagttga taattcactg gccgtcgttt tacggtacca tcgatgatga
     7081 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     7141 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     7201 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     7261 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     7321 tctagatcag atctgcgaag atacggccac gggtgctctt gatcctgtgg ctgattttgg
     7381 actgtgctgc tcgcagctgc tgatgaatca catacttcct ccattttctt ccactgattg
     7441 actgttataa tttccctaat ttccaggtca aggtgctgtg cattgtggta atagatgtga
     7501 catgacgtca cttccaaagg accaatgaac atgtctgacc aatttcatat aatgtgaaaa
     7561 cgattttcat aggcagaata aataacattt aaattaaact gggcatcagc gcaattcaat
     7621 tggtttggta atagcaaggg aaaatagaat gaagtgatct ccaaaaaata agtacttttt
     7681 gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa
     7741 gtaaaagtaa aagtattgat ttttaattgt actcaagtaa agtaaaaatc cccaaaaata
     7801 atacttaagt acagtaatca agtaaaatta ctcaagtact ttacacctct ggttcttgac
     7861 cccctacctt cagcaagccc agcagatcca ctagttctag agcggccgcc accgcggtgg
     7921 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     7981 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8041 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8101 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8161 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8221 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8281 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8341 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8401 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8461 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8521 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8581 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8641 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8701 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8761 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     8821 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8881 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8941 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9001 gctcagtgga acgaaaactc acgttaaggc attttggtca tgagattatc aaaaaggatc
     9061 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9121 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9181 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9241 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9301 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9361 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9421 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9481 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9541 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9601 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9661 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9721 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9781 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9841 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9901 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9961 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10021 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10081 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
    10141 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
    10201 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
    10261 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
    10321 gaaaaaccgt ctatcagggc gatggcccac gctggctaag aactcatcag cctccccggt
    10381 ccatctaccc acgtaccaat gcaccaattg gccacaatga cggctactac atggtgccat
    10441 tccttcctct ttataggaat ggagactacc tcctgtccaa caatgctctt ggatacgagt
    10501 acgcctacct gttggaccca ggtcattgca caacaccaga aatgccctct gatctgcaaa
    10561 agacgtgaat atctgttcag acacccatat ccactctgtt ccacacaggt cagaggtttg
    10621 tccaggagtt cttgacagag gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac
    10681 aagtacttag ggaaaatttt actcaattaa aagtaaaagt atctggctag aatcttactt
    10741 gagtaaaagt aaaaaagtac tccattaaaa ttgtacttga gtattaagga agtaaaagta
    10801 aaagcaagaa agaaaactag agattcttgt ttaagctttt aatctcaaaa aacattaaat
    10861 gaaatgcata caaggtttta tcctgcttta gaactgtttg tatttaatta tcaaactata
    10921 agacagacaa tctaatgcca gtacacgcta ctcaaagttg taaaacctca gatttaactt
    10981 cagtagaagc tgattctcaa aattgttagt gtcaagccta gctcttttgg ggctgaaaag
    11041 caatcctgca gtgctgaaaa gcctctcaca ggcagccgat gcgggaagag gtgtattagt
    11101 cttgatagag aggctgcaaa tagcaggaaa cgtgagcaga gactccctgg tgtctgaaac
    11161 acaggccaga tgggccctcg agcaggaaac agctatgacc atgattacgc caagctatca
    11221 actttgtata gaaaagttgg cacgcgcaga ttccgtgtgg gcctggtaat atcttaccat
    11281 tttaagctat tttaagatta gggaaaaaaa ttaaataaaa ctactttaaa attactttcc
    11341 ttaaaaagca acatatttat ttagtttttg tttaactggc tcaagattgt aatattttac
    11401 ttttacatgt ttttttaagt acctaaatgt gttcaaaagt aaagtatgtt attgtttttt
    11461 ttttttaatg ttagctaagc tttaaattgt aatatattac ttttaaaagt gactttttaa
    11521 aaatgtaaat aaaaaagtaa ctataaatgt gtaatgcatg ttatttctgt gggtttttaa
    11581 ttttcatctc aactgggctt acaattttaa cataataaaa atcaaatcaa atcaaatcag
    11641 gcccttattt gtcacataca ctcttgtata ctgaaataaa ctaaatgttg agaaaaattt
    11701 aaatacacca aaataacgca tacctttttt gtaattacct aagtaaaaca tttagttcct
    11761 tttttaagtg actcaagatt gtaatctatt aattgtaaaa gataaaacta gtgatcaatc
    11821 tgacgtgctt ctatgcagcc tgtgagatta attgtgatgt ttaattatta aacaatttaa
    11881 atggatcaaa catttcacat gtgcttattg tgtccaacaa aaccagcaga agcacttaaa
    11941 aatattgcat gacaacatga gtttttaatg taaattatat atacttccta aacgaagtta
    12001 atcatatttc taaaataata atgtcattga aatatttaaa taaatgataa gctttagttg
    12061 attcaacata aaattgaaag caataaggac tttttttttt tacagcggtg aagcaaaagc
    12121 atgtaataat catataatat aataaataaa taaataaata atggctcttg gtgcaaatat
    12181 gaacacttta aataaatata gaataaatat gatcaataag tcataacgat gtgttttatg
    12241 ttgattataa tttaaacatg tttctaacgc aagttttaaa aactgtttta aagtattatt
    12301 ttaagttata agatcaattt gactcaacat tttgtagtga aaatatgttg tgtaaaatta
    12361 atataatata cactataaaa atgcagggtt ccacacaata cattaatgtt gtcccaacac
    12421 aaatcgatta agttaacaaa tatatgtgga ttgaacataa aaaattaagt tgtcccaatg
    12481 aaatctcaag aattgtgttg tttcagctca ttttaaataa gtagttttaa caagtaaaaa
    12541 ataacatatt tttgagtgtg tatatatata tattacaaaa tataataata taaaatgtaa
    12601 aagatataaa ccatgctaca tatatttaag agctattttg cacattactt ttttgcaagc
    12661 cattttagtg ttttttttac aaatatatat tagctaaata tacaatatct taaatataca
    12721 attaatcaga catgcaatta atgcatttaa cctacataaa aatacatgat caacatatgt
    12781 tttatgctga ttacaactaa atcaaacgta atgaaattaa tttataaaag cgatttaaat
    12841 atgtcaatat tttaagttaa aatcacttga aacctactat aaatgaactt aaattaaaaa
    12901 aatctccttt aattccaatg caacagtgtt gttttttcag ctgaggaccc ccggtgcaga
    12961 tggttggccc ccggcccgct attctaagga agcctttctt aattttaggg cggcgctgca
    13021 tgcgctcaga tcagattcac gggtggagag ggttattttc tcttttttat tttttcttgc
    13081 ctgctcatat ttgggcctgt gtgaacccca ggctccgcct cggcccccag gagccgctct
    13141 tatttaaagg ggatcctcgc cttcagtctt cgtcccagtg gaagcatcag tcttgtgata
    13201 acaacttggt gagtcctaca gaccagcgct cgactctagg ctttaaatgc attgatttct
    13261 ctggattagc taccgggaga gttttaactt tgcttattag tcttgtgctc gaactgctag
    13321 gtttaaagag tgttttctct aatgcatgca tgaagcgaca gctgaaaacc agaaatagag
    13381 cgtttgtcct cctgttaaac tctagccttt ctttggcatt tgtcagtccg agtcttcagc
    13441 tgccggcagg agattgggtt tcagtaacac ttgctgagca caaactacac gtgctttctt
    13501 cagatttaat tcattattaa tcattctaga tttaaatgat gttcttactc attgctgatt
    13561 attttagcct tgcggagcag attaaatgag cacgtgtttc attaagattg atttaaatat
    13621 aattaatatt tcaccactgt agttttaaaa ttgccttcta agtcatggtt gatttttaat
    13681 aggtatcggc tctaattaga ttactatttt ggtttttaaa tgttatttct atttcatttg
    13741 taggctcgtt tttaatgtga ttttattaga ttctagtttt aaaatcatct cttagcctta
    13801 atgagcagac tgaatgaaca tgtttcatta agattaactt cattataatg aattatttaa
    13861 tcattctagt gggttataaa tcagctctat ttaaattaat tgtcgtgttt atttctattt
    13921 aatttttgat tattttttca gggtcgtttt tatgtgattt tttcccgttt ctaattttaa
    13981 actaattgtt tgatttttat atgattcgac tctttttaaa tgaattgttt tgcttttttt
    14041 attgagcata tatatattat acaatttcaa tcaacaattg ttgtgctttt taaatgttgt
    14101 ttctatttca tttctagggt cgattttatt cgattctagt tttaaaatcg tgatttttaa
    14161 cttctaacct tactgagcag actgaatgaa catgtttcat caagattaac ttcattatag
    14221 ttaattattg aatcgtgcta gtggtttata aatcagctct atttaaatta atcatcatca
    14281 ttatttctat ttaattttta ttatttcttt aggattattt ttatgtgatt tttttcacgt
    14341 tctagtttta aactcaatgc tcattactgc tcacccttgc tgagcagtct ttcgttaaga
    14401 ttaaattaat taataaccat tctagtttta aaacagtgtc ttaaggcata gctgatattt
    14461 tacaagttta ctatttaata tatctccttt ctttttttag ggtcgatttc atctgatttg
    14521 attaattttt attatcttca ctaagctttt tgttctaggt tttattctca gtattattac
    14581 tgttatattt gaactgaatt tctcctcata gtttattcat tttatttatt tattttttgt
    14641 ttattcgcta ttttgtattt tagtttttaa ttagaaattt tattagaatc gttttgaact
    14701 agtttttatg cactctaaca ttaatattaa atcataacta ttatttttat tataatttat
    14761 ttgtgtttat ctttttgtgt agctttcatg tgtttttatt ttagttttat tatgtttgca
    14821 ttgaattttt tggactagtt tatataacga tatacagaaa agccatgaca acaaatgttt
    14881 ttataatacc tattaagtat aagctaatca gatttccact attctgttca aagttaacat
    14941 tattagccat tctgatggat catttgacat ttgtcatatg tctttatgaa cactgctctt
    15001 tcagatgcca cttttaaagc tgttttttac agtaaatgaa cgtttattat tatgacatta
    15061 tttcctaagg cctctagagt acagcaggat gtggcgtgtg cattaccgtg taattatgct
    15121 gactgtaaat gtgcatttat agactgtcag catgtctgac tgtgcgctgt tgtgattggt
    15181 cctgcacaga ccaag
//
