LOCUS       sequence-219925-        7903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of OCTN2 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Czeredys M, Samluk L, Michalec K, Tulodziecka K, Skowronek K, Nalecz
            KA
  TITLE     Caveolin-1--a novel interacting partner of organic cation/carnitine 
            transporter (Octn2): effect of protein kinase C on this interaction 
            in rat astrocytes.
  JOURNAL   PLoS One. 2013 Dec 13;8(12):e82105. doi: 
            10.1371/journal.pone.0082105. eCollection 2013.
  PUBMED    24349196
REFERENCE   2  (bases 1 to 7903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-13"; volume: "8(12):e82105. doi"; pages: " 
            10.1371/journal.pone.0082105. eCollection 2013"
COMMENT     Sequence Label: p3 FLAG_CMV14/OCTN2 (delta14_22)
FEATURES             Location/Qualifiers
     source          1..7903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2269..2334
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    2345..2967
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2544..2563)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2991..3011)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3009..3306
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3360..4154
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3414..3433)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4024..4043
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4806..4940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4843..4862)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4897..4916
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4945..4964)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4946..4964)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4978..4994)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4978..4994)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4991..5013)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5002..5018
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5026..5032,5033..5050,5051..5056))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5071..5092
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5209..5226)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5380..5968)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5460..5479)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6139..6930,6931..6999))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6762..6781
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7000..7104)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7131..7586
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7218..7237)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7428..7449
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7712..7734
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7726..7743
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7727..7743
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattaagc ttatgcggga ctacgacgag gtgaccgcct tcctgggcga attcctgctc
      661 agcgccagca tcatccccaa tggcttcaat ggtatgtcta tcgtgttcct ggcggggacc
      721 ccggagcacc gttgccttgt gcctcacacc gtgaacttga gcagcgcgtg gcgcaaccac
      781 agtatcccgt tggagacgaa ggacggacga caggtgcctc agagctgccg ccgctaccga
      841 ctggccacca tcgccaactt ctctgcgctg gggctggagc cgggacggga cgtggacctg
      901 gagcagctgg agcaagagaa ctgcctggat ggctgggagt acaacaagga cgtcttcctg
      961 tccaccatcg tgacagagtg ggacctggtg tgtaaggatg actggaaagc cccactcacc
     1021 acctccttgt ttttcgtggg tgtgctgatg ggctccttca tttcgggaca gctctcagac
     1081 aggtttggtc gcaagaatgt gctgtttttg accatgggca tgcagactgg cttcagcttc
     1141 ctgcagctct tctctgtgaa cttcgagatg tttacagtgc tttttgtcct tgttggcatg
     1201 ggtcagatct ccaactacgt ggcagcattt gtcctgggaa cagaaattct ttccaagtca
     1261 attcgaatta tattcgccac cttaggagtt tgcatatttt atgcatttgg cttcatggtg
     1321 ctgcctctgt ttgcatactt catcagagac tggaggatgc tgctgcttgc gctcactgta
     1381 ccaggggtgc tgtgtggggc tctctggtgg ttcatccctg agtccccccg atggctcatc
     1441 tctcaaggcc gagttaaaga ggcagaggtg atcatccgca aagctgccaa attcaatggg
     1501 atagttgcgc cttccactat cttcgatccc agtgagttac aagacttaaa ttccaagaag
     1561 cctcagtcgc accacattta tgatctggtc cgaacacgaa atatcaggat catcaccatc
     1621 atgtccataa tcctgtggct gaccatatca gtgggctact tcggactttc tcttgacact
     1681 cctaacttgc atggggacat ctatgtgaac tgcttcctac tggcagctgt tgaagtcccc
     1741 gcctatgtgt tggcctggct gttgctacag cacctgcccc ggcggtattc tatctctgct
     1801 gccctcttcc tgggtggcag tgtccttctc ttcatacagc tggtgccttc agaattgttt
     1861 tacttgtcca ctgccctggt gatggtgggc aagtttggaa tcacctctgc ctattccatg
     1921 gtctatgtgt acacggccga gctgtacccc actgtggtca gaaacatggg tgtgggggtc
     1981 agctccacag catcccgcct tggcagcatc ctgtctccct actttgtcta cctcggtgcc
     2041 tatgatcgct tcctgcctta tatcctcatg ggaagtctga ccatcctgac agctatcctc
     2101 accttgttct tcccagagag ctttggtgcc cctctccccg acaccattga ccagatgctc
     2161 agggtcaaag gaataaaaca atggcaaatc caaagccaaa caaggacgca aaaagatggt
     2221 ggagaaagcc caacggtcct aaagagcaca gccttcggat cccgggctga ctacaaagac
     2281 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagtga
     2341 tcccgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac
     2401 tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt
     2461 gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa
     2521 gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc
     2581 ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga
     2641 gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag
     2701 acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc
     2761 accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct
     2821 tctgatttta aaataactat accagcagga ggacgtccag acacagcata ggctacctgg
     2881 ccatgcccaa ccggtgggac atttgagttg cttgcttggc actgtcctct catgcgttgg
     2941 gtccactcag tagatgcctg ttgaattggg tacgcggcca gcttggctgt ggaatgtgtg
     3001 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3061 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3121 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     3181 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     3241 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     3301 ttttggagga attgatcagc ttgggatctg atcaagagac aggatgagga tcgtttcgca
     3361 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     3421 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     3481 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3541 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3601 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3661 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3721 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3781 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3841 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3901 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3961 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     4021 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     4081 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     4141 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     4201 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     4261 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     4321 ccaccccggg ctcgatcccc tcgcgagttg gttcagctgc tgcctgaggc tggacgacct
     4381 cgcggagttc taccggcagt gcaaatccgt cggcatccag gaaaccagca gcggctatcc
     4441 gcgcatccat gcccccgaac tgcaggagtg gggaggcacg atggccgctt tggtcgaccc
     4501 ggacgggacg ctcctgcgcc tgatacagaa cgaattgctt gcaggcatct catgagtgtg
     4561 tcttcccgtt ttccgcctga ggtcactgcg tggatggagc gctggcgcct gctgcgcgac
     4621 ggcgagctgc tcaccaccca ctcgccaagc tggaaccgta aaaaggccgc gttgctggcg
     4681 tttttccata ggctccgccg atcataatca gccataccac atttgtagag gttttacttg
     4741 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     4801 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4861 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4921 tatcttatca tgtctggatc aattccctat agtgagtcgt attaaattcg taatcatgtc
     4981 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5041 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5101 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5161 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5221 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5281 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5341 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5401 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5461 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5521 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5581 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5641 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5701 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5761 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5821 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5881 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5941 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6001 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6061 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6121 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6181 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6241 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6301 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6361 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6421 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6481 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6541 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6601 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6661 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6721 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6781 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6841 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6901 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6961 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7021 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7081 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg
     7141 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7201 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7261 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7321 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7381 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7441 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     7501 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     7561 taacaaaata ttaacgctta caatttccat tcgccattca ggctgcgcaa ctgttgggaa
     7621 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     7681 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     7741 agtgccaagc tgatctatac attgaatcaa tattggcaat tagccatatt agtcattggt
     7801 tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat
     7861 atgtacattt atattggctc atgtccaata tgaccgccat gtt
//
