LOCUS       sequence-219931-        9671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to telethonin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9671)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-TCAP-mKate2
FEATURES             Location/Qualifiers
     source          1..9671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(570..594)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             596..1291
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
     polyA_signal    1413..1494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1495..1515)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1523..1539)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1555..1689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1579..1598)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(2128..2315,2316..2327)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2361..2377)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2361..2377)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2414..2434)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2414..2432)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2453..2469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2466..2488)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2477..2493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2501..2507,2508..2525,2526..2531))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2546..2567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2684..2701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2855..3443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2935..2954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3614..4405,4406..4474))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4237..4256
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4475..4579)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4742..4763)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5112..5123,5124..5286)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5659..5675
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5695..5715
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catgcaggtt tgctcagtcc tggagaagaa
       61 aggaggctgt gtggttggag ccgagctcag ctgcagcgtc agggaggaaa acccaaataa
      121 gagagagagt tacaccgctg actggcgcag catcaatatg aagacacaac ctgaggatcg
      181 ccagtcgatg ctgatgtcag acgattctcg gcgagagtct ctatcccgtt attggcaggt
      241 gcgtcctctt catcaggcct gtccgtcggg tgtgcgcaga gcgggcaccg tggacactgg
      301 tgtaagagag caccagctct tgccctacag gaacagcctg ccccttccca tcttcaagcc
      361 tgcagaactg ggcgtccgcc tgggtcgtgg agctccacac accctggagg atctgccacc
      421 tgtccgcatc cctgacggag tctgtccaga caagagagca gtggaacaga tcaccagaga
      481 cctgccgcct gtcaaaccca tgcggatgga gttcgccaag gcacccagag ctctgggacg
      541 atcaatgtct caggaggccc agagaggcga cccagctttc ttgtacaaag tggctgtgag
      601 cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca
      661 ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
      721 aatcaaggcg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt
      781 catgtacggc agcaaaacct tcatcaacca cacccagggc atccccgact tctttaagca
      841 gtccttcccc gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
      901 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
      961 aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
     1021 ctccaccgag accctgtacc ccgctgacgg cggcctggaa ggcagagccg acatggccct
     1081 gaagctcgtg ggcgggggcc acctgatctg caacttgaag accacataca gatccaagaa
     1141 acccgctaag aacctcaaga tgcccggcgt ctactatgtg gacagaagac tggaaagaat
     1201 caaggaggcc gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg
     1261 cgacctccct agcaaactgg ggcacagatg agcggccgcg actctagatc ataatcagcc
     1321 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     1381 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     1441 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgccaactt
     1501 tattatacat agttgataat tcactggccg tcgttttacg gtaccatcga tgatgatcca
     1561 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     1621 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     1681 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     1741 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcctcta
     1801 gatcagatct gcgaagatac ggccacgggt gctcttgatc ctgtggctga ttttggactg
     1861 tgctgctcgc agctgctgat gaatcacata cttcctccat tttcttccac tgattgactg
     1921 ttataatttc cctaatttcc aggtcaaggt gctgtgcatt gtggtaatag atgtgacatg
     1981 acgtcacttc caaaggacca atgaacatgt ctgaccaatt tcatataatg tgaaaacgat
     2041 tttcataggc agaataaata acatttaaat taaactgggc atcagcgcaa ttcaattggt
     2101 ttggtaatag caagggaaaa tagaatgaag tgatctccaa aaaataagta ctttttgact
     2161 gtaaataaaa ttgtaaggag taaaaagtac ttttttttct aaaaaaatgt aattaagtaa
     2221 aagtaaaagt attgattttt aattgtactc aagtaaagta aaaatcccca aaaataatac
     2281 ttaagtacag taatcaagta aaattactca agtactttac acctctggtt cttgaccccc
     2341 taccttcagc aagcccagca gatccactag ttctagagcg gccgccaccg cggtggagct
     2401 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     2461 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     2521 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2581 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     2641 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     2701 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2761 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2821 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2881 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2941 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3001 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3061 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3121 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3181 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3241 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3301 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3361 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3421 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3481 agtggaacga aaactcacgt taaggcattt tggtcatgag attatcaaaa aggatcttca
     3541 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3601 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3661 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3721 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3781 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3841 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3901 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3961 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4021 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4081 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4141 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4201 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4261 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4321 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4381 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4441 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4501 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4561 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
     4621 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     4681 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     4741 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     4801 aaccgtctat cagggcgatg gcccacgctg gctaagaact catcagcctc cccggtccat
     4861 ctacccacgt accaatgcac caattggcca caatgacggc tactacatgg tgccattcct
     4921 tcctctttat aggaatggag actacctcct gtccaacaat gctcttggat acgagtacgc
     4981 ctacctgttg gacccaggtc attgcacaac accagaaatg ccctctgatc tgcaaaagac
     5041 gtgaatatct gttcagacac ccatatccac tctgttccac acaggtcaga ggtttgtcca
     5101 ggagttcttg acagaggtgt aaaaagtact caaaaatttt actcaagtga aagtacaagt
     5161 acttagggaa aattttactc aattaaaagt aaaagtatct ggctagaatc ttacttgagt
     5221 aaaagtaaaa aagtactcca ttaaaattgt acttgagtat taaggaagta aaagtaaaag
     5281 caagaaagaa aactagagat tcttgtttaa gcttttaatc tcaaaaaaca ttaaatgaaa
     5341 tgcatacaag gttttatcct gctttagaac tgtttgtatt taattatcaa actataagac
     5401 agacaatcta atgccagtac acgctactca aagttgtaaa acctcagatt taacttcagt
     5461 agaagctgat tctcaaaatt gttagtgtca agcctagctc ttttggggct gaaaagcaat
     5521 cctgcagtgc tgaaaagcct ctcacaggca gccgatgcgg gaagaggtgt attagtcttg
     5581 atagagaggc tgcaaatagc aggaaacgtg agcagagact ccctggtgtc tgaaacacag
     5641 gccagatggg ccctcgagca ggaaacagct atgaccatga ttacgccaag ctatcaactt
     5701 tgtatagaaa agttggcacg cgcagattcc gtgtgggcct ggtaatatct taccatttta
     5761 agctatttta agattaggga aaaaaattaa ataaaactac tttaaaatta ctttccttaa
     5821 aaagcaacat atttatttag tttttgttta actggctcaa gattgtaata ttttactttt
     5881 acatgttttt ttaagtacct aaatgtgttc aaaagtaaag tatgttattg tttttttttt
     5941 ttaatgttag ctaagcttta aattgtaata tattactttt aaaagtgact ttttaaaaat
     6001 gtaaataaaa aagtaactat aaatgtgtaa tgcatgttat ttctgtgggt ttttaatttt
     6061 catctcaact gggcttacaa ttttaacata ataaaaatca aatcaaatca aatcaggccc
     6121 ttatttgtca catacactct tgtatactga aataaactaa atgttgagaa aaatttaaat
     6181 acaccaaaat aacgcatacc ttttttgtaa ttacctaagt aaaacattta gttccttttt
     6241 taagtgactc aagattgtaa tctattaatt gtaaaagata aaactagtga tcaatctgac
     6301 gtgcttctat gcagcctgtg agattaattg tgatgtttaa ttattaaaca atttaaatgg
     6361 atcaaacatt tcacatgtgc ttattgtgtc caacaaaacc agcagaagca cttaaaaata
     6421 ttgcatgaca acatgagttt ttaatgtaaa ttatatatac ttcctaaacg aagttaatca
     6481 tatttctaaa ataataatgt cattgaaata tttaaataaa tgataagctt tagttgattc
     6541 aacataaaat tgaaagcaat aaggactttt tttttttaca gcggtgaagc aaaagcatgt
     6601 aataatcata taatataata aataaataaa taaataatgg ctcttggtgc aaatatgaac
     6661 actttaaata aatatagaat aaatatgatc aataagtcat aacgatgtgt tttatgttga
     6721 ttataattta aacatgtttc taacgcaagt tttaaaaact gttttaaagt attattttaa
     6781 gttataagat caatttgact caacattttg tagtgaaaat atgttgtgta aaattaatat
     6841 aatatacact ataaaaatgc agggttccac acaatacatt aatgttgtcc caacacaaat
     6901 cgattaagtt aacaaatata tgtggattga acataaaaaa ttaagttgtc ccaatgaaat
     6961 ctcaagaatt gtgttgtttc agctcatttt aaataagtag ttttaacaag taaaaaataa
     7021 catatttttg agtgtgtata tatatatatt acaaaatata ataatataaa atgtaaaaga
     7081 tataaaccat gctacatata tttaagagct attttgcaca ttactttttt gcaagccatt
     7141 ttagtgtttt ttttacaaat atatattagc taaatataca atatcttaaa tatacaatta
     7201 atcagacatg caattaatgc atttaaccta cataaaaata catgatcaac atatgtttta
     7261 tgctgattac aactaaatca aacgtaatga aattaattta taaaagcgat ttaaatatgt
     7321 caatatttta agttaaaatc acttgaaacc tactataaat gaacttaaat taaaaaaatc
     7381 tcctttaatt ccaatgcaac agtgttgttt tttcagctga ggacccccgg tgcagatggt
     7441 tggcccccgg cccgctattc taaggaagcc tttcttaatt ttagggcggc gctgcatgcg
     7501 ctcagatcag attcacgggt ggagagggtt attttctctt ttttattttt tcttgcctgc
     7561 tcatatttgg gcctgtgtga accccaggct ccgcctcggc ccccaggagc cgctcttatt
     7621 taaaggggat cctcgccttc agtcttcgtc ccagtggaag catcagtctt gtgataacaa
     7681 cttggtgagt cctacagacc agcgctcgac tctaggcttt aaatgcattg atttctctgg
     7741 attagctacc gggagagttt taactttgct tattagtctt gtgctcgaac tgctaggttt
     7801 aaagagtgtt ttctctaatg catgcatgaa gcgacagctg aaaaccagaa atagagcgtt
     7861 tgtcctcctg ttaaactcta gcctttcttt ggcatttgtc agtccgagtc ttcagctgcc
     7921 ggcaggagat tgggtttcag taacacttgc tgagcacaaa ctacacgtgc tttcttcaga
     7981 tttaattcat tattaatcat tctagattta aatgatgttc ttactcattg ctgattattt
     8041 tagccttgcg gagcagatta aatgagcacg tgtttcatta agattgattt aaatataatt
     8101 aatatttcac cactgtagtt ttaaaattgc cttctaagtc atggttgatt tttaataggt
     8161 atcggctcta attagattac tattttggtt tttaaatgtt atttctattt catttgtagg
     8221 ctcgttttta atgtgatttt attagattct agttttaaaa tcatctctta gccttaatga
     8281 gcagactgaa tgaacatgtt tcattaagat taacttcatt ataatgaatt atttaatcat
     8341 tctagtgggt tataaatcag ctctatttaa attaattgtc gtgtttattt ctatttaatt
     8401 tttgattatt ttttcagggt cgtttttatg tgattttttc ccgtttctaa ttttaaacta
     8461 attgtttgat ttttatatga ttcgactctt tttaaatgaa ttgttttgct ttttttattg
     8521 agcatatata tattatacaa tttcaatcaa caattgttgt gctttttaaa tgttgtttct
     8581 atttcatttc tagggtcgat tttattcgat tctagtttta aaatcgtgat ttttaacttc
     8641 taaccttact gagcagactg aatgaacatg tttcatcaag attaacttca ttatagttaa
     8701 ttattgaatc gtgctagtgg tttataaatc agctctattt aaattaatca tcatcattat
     8761 ttctatttaa tttttattat ttctttagga ttatttttat gtgatttttt tcacgttcta
     8821 gttttaaact caatgctcat tactgctcac ccttgctgag cagtctttcg ttaagattaa
     8881 attaattaat aaccattcta gttttaaaac agtgtcttaa ggcatagctg atattttaca
     8941 agtttactat ttaatatatc tcctttcttt ttttagggtc gatttcatct gatttgatta
     9001 atttttatta tcttcactaa gctttttgtt ctaggtttta ttctcagtat tattactgtt
     9061 atatttgaac tgaatttctc ctcatagttt attcatttta tttatttatt ttttgtttat
     9121 tcgctatttt gtattttagt ttttaattag aaattttatt agaatcgttt tgaactagtt
     9181 tttatgcact ctaacattaa tattaaatca taactattat ttttattata atttatttgt
     9241 gtttatcttt ttgtgtagct ttcatgtgtt tttattttag ttttattatg tttgcattga
     9301 attttttgga ctagtttata taacgatata cagaaaagcc atgacaacaa atgtttttat
     9361 aatacctatt aagtataagc taatcagatt tccactattc tgttcaaagt taacattatt
     9421 agccattctg atggatcatt tgacatttgt catatgtctt tatgaacact gctctttcag
     9481 atgccacttt taaagctgtt ttttacagta aatgaacgtt tattattatg acattatttc
     9541 ctaaggcctc tagagtacag caggatgtgg cgtgtgcatt accgtgtaat tatgctgact
     9601 gtaaatgtgc atttatagac tgtcagcatg tctgactgtg cgctgttgtg attggtcctg
     9661 cacagaccaa g
//
