LOCUS       sequence-219933-        7879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of OCTN2 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Czeredys M, Samluk L, Michalec K, Tulodziecka K, Skowronek K, Nalecz
            KA
  TITLE     Caveolin-1--a novel interacting partner of organic cation/carnitine 
            transporter (Octn2): effect of protein kinase C on this interaction 
            in rat astrocytes.
  JOURNAL   PLoS One. 2013 Dec 13;8(12):e82105. doi: 
            10.1371/journal.pone.0082105. eCollection 2013.
  PUBMED    24349196
REFERENCE   2  (bases 1 to 7879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-13"; volume: "8(12):e82105. doi"; pages: " 
            10.1371/journal.pone.0082105. eCollection 2013"
COMMENT     Sequence Label: p3 FLAG_CMV14/OCTN2 (delta14_22/delta447_454)
FEATURES             Location/Qualifiers
     source          1..7879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2245..2310
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    2321..2943
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2520..2539)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2967..2987)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2985..3282
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3336..4130
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3390..3409)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4000..4019
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4782..4916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4819..4838)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4873..4892
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4921..4940)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4922..4940)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4954..4970)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4954..4970)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4967..4989)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4978..4994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5002..5008,5009..5026,5027..5032))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5047..5068
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5185..5202)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5356..5944)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5436..5455)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6115..6906,6907..6975))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6738..6757
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6976..7080)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7107..7562
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7194..7213)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7404..7425
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7688..7710
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7702..7719
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7703..7719
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattaagc ttatgcggga ctacgacgag gtgaccgcct tcctgggcga attcctgctc
      661 agcgccagca tcatccccaa tggcttcaat ggtatgtcta tcgtgttcct ggcggggacc
      721 ccggagcacc gttgccttgt gcctcacacc gtgaacttga gcagcgcgtg gcgcaaccac
      781 agtatcccgt tggagacgaa ggacggacga caggtgcctc agagctgccg ccgctaccga
      841 ctggccacca tcgccaactt ctctgcgctg gggctggagc cgggacggga cgtggacctg
      901 gagcagctgg agcaagagaa ctgcctggat ggctgggagt acaacaagga cgtcttcctg
      961 tccaccatcg tgacagagtg ggacctggtg tgtaaggatg actggaaagc cccactcacc
     1021 acctccttgt ttttcgtggg tgtgctgatg ggctccttca tttcgggaca gctctcagac
     1081 aggtttggtc gcaagaatgt gctgtttttg accatgggca tgcagactgg cttcagcttc
     1141 ctgcagctct tctctgtgaa cttcgagatg tttacagtgc tttttgtcct tgttggcatg
     1201 ggtcagatct ccaactacgt ggcagcattt gtcctgggaa cagaaattct ttccaagtca
     1261 attcgaatta tattcgccac cttaggagtt tgcatatttt atgcatttgg cttcatggtg
     1321 ctgcctctgt ttgcatactt catcagagac tggaggatgc tgctgcttgc gctcactgta
     1381 ccaggggtgc tgtgtggggc tctctggtgg ttcatccctg agtccccccg atggctcatc
     1441 tctcaaggcc gagttaaaga ggcagaggtg atcatccgca aagctgccaa attcaatggg
     1501 atagttgcgc cttccactat cttcgatccc agtgagttac aagacttaaa ttccaagaag
     1561 cctcagtcgc accacattta tgatctggtc cgaacacgaa atatcaggat catcaccatc
     1621 atgtccataa tcctgtggct gaccatatca gtgggctact tcggactttc tcttgacact
     1681 cctaacttgc atggggacat ctatgtgaac tgcttcctac tggcagctgt tgaagtcccc
     1741 gcctatgtgt tggcctggct gttgctacag cacctgcccc ggcggtattc tatctctgct
     1801 gccctcttcc tgggtggcag tgtccttctc ttcatacagc tggtgccttc agaattgttt
     1861 tacttgtcca ctgccctggt gatggtgggc aagtttggaa tcacctctgc ctattccatg
     1921 gtacccactg tggtcagaaa catgggtgtg ggggtcagct ccacagcatc ccgccttggc
     1981 agcatcctgt ctccctactt tgtctacctc ggtgcctatg atcgcttcct gccttatatc
     2041 ctcatgggaa gtctgaccat cctgacagct atcctcacct tgttcttccc agagagcttt
     2101 ggtgcccctc tccccgacac cattgaccag atgctcaggg tcaaaggaat aaaacaatgg
     2161 caaatccaaa gccaaacaag gacgcaaaaa gatggtggag aaagcccaac ggtcctaaag
     2221 agcacagcct tcggatcccg ggctgactac aaagaccatg acggtgatta taaagatcat
     2281 gacatcgact acaaggatga cgatgacaag tagtgatccc gggtggcatc cctgtgaccc
     2341 ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct
     2401 aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg
     2461 gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt
     2521 ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc
     2581 ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat
     2641 gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag
     2701 gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg
     2761 attacaggcg tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca
     2821 gcaggaggac gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt
     2881 gagttgcttg cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga
     2941 attgggtacg cggccagctt ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3001 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3061 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3121 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3181 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3241 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggaattg atcagcttgg
     3301 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     3361 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     3421 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     3481 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     3541 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     3601 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     3661 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     3721 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     3781 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     3841 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     3901 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     3961 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     4021 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     4081 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     4141 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     4201 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     4261 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccgggctcg atcccctcgc
     4321 gagttggttc agctgctgcc tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa
     4381 atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc atccatgccc ccgaactgca
     4441 ggagtgggga ggcacgatgg ccgctttggt cgacccggac gggacgctcc tgcgcctgat
     4501 acagaacgaa ttgcttgcag gcatctcatg agtgtgtctt cccgttttcc gcctgaggtc
     4561 actgcgtgga tggagcgctg gcgcctgctg cgcgacggcg agctgctcac cacccactcg
     4621 ccaagctgga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgccgatca
     4681 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     4741 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     4801 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4861 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaatt
     4921 ccctatagtg agtcgtatta aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     4981 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     5041 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     5101 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     5161 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     5221 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     5281 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     5341 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     5401 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5461 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     5521 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     5581 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5641 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5701 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5761 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     5821 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5881 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5941 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     6001 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     6061 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     6121 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     6181 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6241 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     6301 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     6361 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6421 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6481 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6541 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6601 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6661 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6721 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6781 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6841 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6901 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6961 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     7021 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     7081 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     7141 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7201 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7261 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7321 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7381 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7441 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7501 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     7561 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     7621 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     7681 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctgat ctatacattg
     7741 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc
     7801 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
     7861 ccaatatgac cgccatgtt
//
