LOCUS       sequence-219935-       10035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable TCR expression in human T cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10035)
  AUTHORS   Wang R, Pscheid R, Ghumra A, Kan LYL, Cochrane S, Fairclough L, 
            Alcocer MJC
  TITLE     Towards a surrogate system to express human lipid binding TCRs.
  JOURNAL   Biotechnol Lett. 2019 Oct;41(10):1095-1104. doi: 
            10.1007/s10529-019-02713-2. Epub 2019 Jul 26.
  PUBMED    31346817
REFERENCE   2  (bases 1 to 10035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s10529-019-02713-2"; journalName: "Biotechnol Lett"; date: 
            "2019-10"; volume: "41"; issue: "10"; pages: "1095-1104"
COMMENT     Sequence Label: pMJA285= pSin-TCRab-CMVp-Puro
FEATURES             Location/Qualifiers
     source          1..10035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             117..750
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    797..922
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1415..1648
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1833..1877
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2164..2281
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2428..2639
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        3887..4190
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4191..4394
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4345..4365
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(5596..5615)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5661..5716
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5715..5734)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    join(6151..6725,6726..6728,6729..6737)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6318..6335)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6545..6564
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6757..7356
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(6757..6776)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7253..7273
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7559..7792
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7943..7960)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7949..8173
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      8216..8225
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        8269..8373
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8374..8442,8443..9234)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8592..8611)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9405..9993
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9894..9913
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggaccgcca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
       61 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
      121 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
      181 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
      241 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
      301 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
      361 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
      421 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
      481 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
      541 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      721 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      781 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
      841 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
      901 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
      961 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1021 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1081 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1141 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1201 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1261 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1321 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1381 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1441 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1501 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1561 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1621 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1681 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1741 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     1801 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     1861 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     1921 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     1981 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2041 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2101 gatccattcg attagtgaac ggatctcgac ggtatcgcca caaatggcag tattcatcca
     2161 caattttaaa agaaaggggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2281 tcgggtttat tacagggaca gcagagatcc actttggtcg ataagctttg caaagatgga
     2341 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
     2401 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     2461 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     2521 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     2581 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     2641 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     2701 cttgaattac ttccacctgg ctgcagtaat ttaaaactag atcagctgga ccacagtctc
     2761 agggtcatca gcaggttgaa gccggccacc ttcagcagca ggattctgaa gccgatcacg
     2821 ctcaggttct ggaagttcag gttggtgtct gtctcgaagc tcttttccac cagcttcacg
     2881 tcgcagctgc tctcggggct tgggaagaat gtgtcctcgg ggataatgct gttgttgaag
     2941 gcgttggcgc aggcgaagtc gctcttgttg gaccaggcca cggcgctgtt agacttgaag
     3001 tccatggatc tcatgtccag cacggtcttg tcggtgatgt acacgtcgct gtccttgctc
     3061 tgggacacgt tggtctggct gtcgaagtcg gtgaacaggc acacgctctt gtcgctggac
     3121 ttggagtctc tcagctggta cacggcaggg tcagggttct ggatgtcggg ccacacggtc
     3181 agctgggttc ctctgccgaa gtacagtctg cccagggtgc tgcctctgtc ggacaccacg
     3241 cagatgtagc tggcgctgtc gctcagctgg ctggcggtga tgtgcaggct gctctgcttt
     3301 gtgtcggcgt ccagggtggc ggtgtatctg ccgttgctct tggtgttctc gctgaaggtc
     3361 atgatggtca gggacacggg gcctctgccg gtgtcctgct tgtaccatct caggttgctg
     3421 aatggggaca cggtgtagtt gcactgcagg gtgcagttct tgccttccag gatgatcagg
     3481 ctctgggggc tctgttccac ctggttcttg ccgttgcctc tgtagaagta cagccacagg
     3541 atcacgagaa aggtggtcag gtgcttcttc atggttgttc tggagaagcc tgcctcaaat
     3601 ctgctgcctt gtttcatcag ctgtttcttg taacattaaa cagggactct caggaagtac
     3661 agtcagccaa tcagagaaca gatgtgcaca agcacacaca cacacacaca ggatctgggc
     3721 ccccgcggca tatgaccggt gaattctcca ggcgatctga cggttcacta aacgagctct
     3781 gcttatatag gcctcccacc gtacacgcca cctcgacata ctcgagtagt tattaatagt
     3841 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     3901 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     3961 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     4021 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     4081 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     4141 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     4201 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     4261 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     4321 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     4381 atataagcag agctggttta gtgaaccgtc agatccgcta gggatcctct agtcagctga
     4441 cgcgtgctag cgcggccgca tcgataagct tgtcgagcag agcctattag gagactacag
     4501 atccaaacct ccaaatagtt cccccagaag tcctggaccc tgaacaaggg gatgctgtca
     4561 tgaccatcag actgctgtgc tacgtgggct tctacttcct gggcgctggc ctgatggaag
     4621 ccgacatcta ccagaccccc agatacctcg tgatcggcac cggcaagaaa atcaccctgg
     4681 aatgcagcca gaccatgggc cacgacaaga tgtactggta tcagcaggac cccggcatgg
     4741 aactgcacct gatccactac agctacggcg tgaacagcac cgagaagggc gacctgagca
     4801 gcgagagcac cgtgtccaga atcagaaccg agcacttccc actgaccctg gaaagcgcta
     4861 gacccagcca caccagccag tacctgtgtg ccagcagctc tcctctgggc ggaacacctg
     4921 gcgccaacgt gctgacattt ggcgctggca gcagactgac cgtgctggaa gatctgaaga
     4981 acgtgttccc acccgaggtg gccgtgttcg agccttctga ggccgagatc tcccacaccc
     5041 agaaagccac cctcgtgtgt ctggccaccg gcttctaccc cgaccacgtg gaactgtctt
     5101 ggtgggtcaa cggcaaagag gtgcacagcg gcgtgtccac cgatccccag cctctgaaag
     5161 agcagcccgc cctgaacgac agcagatact gcctgtccag caggctgaga gtgtccgcca
     5221 ccttctggca gaaccccaga aaccacttca gatgccaggt gcagttttac ggcctgagcg
     5281 agaacgacga gtggacccag gacagagcca agcccgtgac ccagatcgtg tctgctgagg
     5341 cctggggcag agccgactgt ggcttcacaa gcgagagcta ccagcagggc gtgctgagcg
     5401 ccaccatcct gtacgagatc ctgctgggca aggccaccct gtacgccgtg ctggtgtctg
     5461 ccctggtgct gatggccatg gtcaagagaa aggacagcag aggctgagaa ttcaattcac
     5521 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     5581 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     5641 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     5701 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     5761 tgagtatttg gtttagagtt tggcaacata tgccatatgc tggctgccat gaacaaaggt
     5821 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     5881 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     5941 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     6001 tactcccagt catagctgtc cctcttctct tactagtaat ttcctaggta gtttaaacaa
     6061 gaaacttttg aattcgaagc ttgagctcga gatctgagtc cggtagcgct agttcgaagg
     6121 atccgcatgc atctagggcg gccaattccg cccctctccc tccccccccc ctaacgttac
     6181 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgtgat tttccaccat
     6241 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     6301 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     6361 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     6421 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     6481 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     6541 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     6601 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     6661 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     6721 ataagcttgc cacaacccac aaggagacga ccttccatga ccgagtacaa gcccacggtg
     6781 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc
     6841 gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag
     6901 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     6961 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     7021 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     7081 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     7141 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     7201 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     7261 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgagtgccc gaaggaccgc
     7321 gcgacctggt gcatgacccg caagcccggt gcctgataat aggcggccgc tcgagaccta
     7381 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     7441 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     7501 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7561 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     7621 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7681 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7741 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     7801 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     7861 aggccttgac attataatag atttagcagg aattgaacta ggagtggagc acacaggcaa
     7921 agttctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     7981 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     8041 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     8101 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     8161 gtgggctcta tggcttctga ggcggaaaga accagctggg ggcgcgcccc tcgaggccgc
     8221 catggtcata gctgtttgac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     8281 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8341 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8401 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     8461 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     8521 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     8581 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     8641 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8701 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8761 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8821 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8881 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     8941 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9001 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9061 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9121 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9181 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9241 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9301 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9361 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9421 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9481 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9541 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9601 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9661 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9721 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9781 tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9841 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9901 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9961 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10021 ttcctggcct tttgc
//
