LOCUS       sequence-219946-       10980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to junctophilin
            1a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-jph1a
FEATURES             Location/Qualifiers
     source          1..10980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2804..2824)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2832..2848)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2864..2998
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2888..2907)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2942..2961
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(3437..3624,3625..3636)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3670..3686)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3670..3686)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3723..3743)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3723..3741)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3762..3778)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3775..3797)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3786..3802
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3810..3816,3817..3834,3835..3840))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3855..3876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3993..4010)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4164..4752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4244..4263)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4923..5714,5715..5783))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5546..5565
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5784..5888)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6051..6072)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(6421..6432,6433..6595)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6968..6984
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7004..7024
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtgacgggc ggacggttcg acttcgacga
      781 tggaggcact tactgtggcg gatgggagga cgggaaagcc cacggacacg gcatctgcac
      841 gggacccaag ggtcagggcg agtactccgg gtcctggtcc cacgggtttg aggtagtcgg
      901 ggtctacacc tggcccagcg ggaacacgta ccagggctac tggtcgcagg ggaagcgcca
      961 cgggctgggt gttgagacca aggggaaatg gctttatcgc ggggagtgga gtcacgggtt
     1021 caagggtcgc tatggagttc ggcagagcac caacacacct gctagatatg atgggacgtg
     1081 gagtaatgga ctgcaggatg gatatggagt cgaaacctat ggggatagtg gcacttatca
     1141 aggccagtgg atggggggca tgaggcatgg ctatggcgtc cgtcagagcg tgccttatgg
     1201 aatggccacc atcatccgtt ctccgctccg cacctccctg gcctctctaa gaagtgtgca
     1261 aagcaacggc gccgttctcc aggacacagt accagacact ccagttggaa gccgtggtgg
     1321 tttcgtgctg aattttcaca gcgacaccga agttgtcacc ggtaagaaga agggcctgtt
     1381 ccgtcgcggg tccctcttcg ggagcctccg ccagctgcgt aaatctgact ccaaaacgtc
     1441 catctctagc aagcgaagct cagcacgtag cgatgccacc atgagccgca tcagctccag
     1501 cgacgccaac tccaccatca gctatggaga aggagaggtg cctgatgatt acacgccaat
     1561 ggaggaccac gtggatgcca ccaccactga gtcctacatg ggcgaatgga agaacgataa
     1621 gcgcaatggg tttggggtca gtgagagatc caacgggctg aagtatgaag gggaatggat
     1681 gaacaacaag aggcatggat acgggtgcac catattccct gatggaacca aagaggaggg
     1741 gaagtataaa aataacgtgt tggtgcgcgg gataaggaag cagctcattc ctcttaaaaa
     1801 ccataaaacg aaggaaaaag tggaccgggc tgtggagggg gctcgaaggt cggcagctat
     1861 cgccaggacg aaagtggaaa tagcagtttc aaggacgtcc cacgcacgga ctaagagtga
     1921 agcagcagac caggccgctc tcgcagcatg tcaggagtca gatttagccc gagctgtggc
     1981 ttgtgaatta tctccaagct tccatcaacc aggactggaa tacatcaagc agaagttcag
     2041 tgagccggtg gaggagatta aagaagaagc acccaaagaa gaggagaaga agtcttcagg
     2101 tagccctttc tataggcgcg ggacgactcc gagccactct ccagcccaaa gcccgacccc
     2161 atcccctccc cgcacctctc agaaaagcca agcgcagaac cccagcatta gccgcaagat
     2221 cagcaaagac aacagcgtaa cggcccgcaa gatcagcaga gacagcgctt ctgtagcccg
     2281 caagatcagc aaagaagaaa gagcctccat tgtggccgaa atcaccaaag tatccgtacc
     2341 tcctcctcct cttcctcctc ctcctcctcc tgcagttcag actatcaagc tgcaggatgt
     2401 cccacctcca aagcctccta aagcacttga acccgtggcc aaacctgcgg gcacaggtaa
     2461 cgggcagctt catactgcct atcacagtta ttatgtgaag ccggtgaagc agttgccacc
     2521 gccagaggag cccgaagatg aggagcctga aatcacacag tccagtctgg ccaggatgcc
     2581 tccacctccg aaaacgctca acacacccac tcccaaacca gaggtcaaga tcaggaaaca
     2641 agagtctatt aaaccaaaga gcttagctga aaccaagaag gccagcttgg acctggttga
     2701 agaagcaacg gtggaagaat ctggtcctaa ctcaattctg gttgccatgg tgatgctttt
     2761 gaatattgga ttggcaatca tttttgtcca tttcctgaca tgacaacttt attatacata
     2821 gttgataatt cactggccgt cgttttacgg taccatcgat gatgatccag acatgataag
     2881 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     2941 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     3001 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     3061 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag atcagatctg
     3121 cgaagatacg gccacgggtg ctcttgatcc tgtggctgat tttggactgt gctgctcgca
     3181 gctgctgatg aatcacatac ttcctccatt ttcttccact gattgactgt tataatttcc
     3241 ctaatttcca ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga cgtcacttcc
     3301 aaaggaccaa tgaacatgtc tgaccaattt catataatgt gaaaacgatt ttcataggca
     3361 gaataaataa catttaaatt aaactgggca tcagcgcaat tcaattggtt tggtaatagc
     3421 aagggaaaat agaatgaagt gatctccaaa aaataagtac tttttgactg taaataaaat
     3481 tgtaaggagt aaaaagtact tttttttcta aaaaaatgta attaagtaaa agtaaaagta
     3541 ttgattttta attgtactca agtaaagtaa aaatccccaa aaataatact taagtacagt
     3601 aatcaagtaa aattactcaa gtactttaca cctctggttc ttgaccccct accttcagca
     3661 agcccagcag atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
     3721 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     3781 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     3841 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     3901 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     3961 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4021 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4081 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4141 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4201 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4261 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4321 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4381 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4441 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4501 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4561 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4621 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4681 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4741 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4801 aactcacgtt aaggcatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4861 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4921 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4981 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5041 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5101 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5161 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5221 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5281 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5341 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5401 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5461 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5521 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5581 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5641 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5701 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5761 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5821 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5881 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     5941 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     6001 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     6061 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     6121 agggcgatgg cccacgctgg ctaagaactc atcagcctcc ccggtccatc tacccacgta
     6181 ccaatgcacc aattggccac aatgacggct actacatggt gccattcctt cctctttata
     6241 ggaatggaga ctacctcctg tccaacaatg ctcttggata cgagtacgcc tacctgttgg
     6301 acccaggtca ttgcacaaca ccagaaatgc cctctgatct gcaaaagacg tgaatatctg
     6361 ttcagacacc catatccact ctgttccaca caggtcagag gtttgtccag gagttcttga
     6421 cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa agtacaagta cttagggaaa
     6481 attttactca attaaaagta aaagtatctg gctagaatct tacttgagta aaagtaaaaa
     6541 agtactccat taaaattgta cttgagtatt aaggaagtaa aagtaaaagc aagaaagaaa
     6601 actagagatt cttgtttaag cttttaatct caaaaaacat taaatgaaat gcatacaagg
     6661 ttttatcctg ctttagaact gtttgtattt aattatcaaa ctataagaca gacaatctaa
     6721 tgccagtaca cgctactcaa agttgtaaaa cctcagattt aacttcagta gaagctgatt
     6781 ctcaaaattg ttagtgtcaa gcctagctct tttggggctg aaaagcaatc ctgcagtgct
     6841 gaaaagcctc tcacaggcag ccgatgcggg aagaggtgta ttagtcttga tagagaggct
     6901 gcaaatagca ggaaacgtga gcagagactc cctggtgtct gaaacacagg ccagatgggc
     6961 cctcgagcag gaaacagcta tgaccatgat tacgccaagc tatcaacttt gtatagaaaa
     7021 gttggcacgc gcagattccg tgtgggcctg gtaatatctt accattttaa gctattttaa
     7081 gattagggaa aaaaattaaa taaaactact ttaaaattac tttccttaaa aagcaacata
     7141 tttatttagt ttttgtttaa ctggctcaag attgtaatat tttactttta catgtttttt
     7201 taagtaccta aatgtgttca aaagtaaagt atgttattgt tttttttttt taatgttagc
     7261 taagctttaa attgtaatat attactttta aaagtgactt tttaaaaatg taaataaaaa
     7321 agtaactata aatgtgtaat gcatgttatt tctgtgggtt tttaattttc atctcaactg
     7381 ggcttacaat tttaacataa taaaaatcaa atcaaatcaa atcaggccct tatttgtcac
     7441 atacactctt gtatactgaa ataaactaaa tgttgagaaa aatttaaata caccaaaata
     7501 acgcatacct tttttgtaat tacctaagta aaacatttag ttcctttttt aagtgactca
     7561 agattgtaat ctattaattg taaaagataa aactagtgat caatctgacg tgcttctatg
     7621 cagcctgtga gattaattgt gatgtttaat tattaaacaa tttaaatgga tcaaacattt
     7681 cacatgtgct tattgtgtcc aacaaaacca gcagaagcac ttaaaaatat tgcatgacaa
     7741 catgagtttt taatgtaaat tatatatact tcctaaacga agttaatcat atttctaaaa
     7801 taataatgtc attgaaatat ttaaataaat gataagcttt agttgattca acataaaatt
     7861 gaaagcaata aggacttttt ttttttacag cggtgaagca aaagcatgta ataatcatat
     7921 aatataataa ataaataaat aaataatggc tcttggtgca aatatgaaca ctttaaataa
     7981 atatagaata aatatgatca ataagtcata acgatgtgtt ttatgttgat tataatttaa
     8041 acatgtttct aacgcaagtt ttaaaaactg ttttaaagta ttattttaag ttataagatc
     8101 aatttgactc aacattttgt agtgaaaata tgttgtgtaa aattaatata atatacacta
     8161 taaaaatgca gggttccaca caatacatta atgttgtccc aacacaaatc gattaagtta
     8221 acaaatatat gtggattgaa cataaaaaat taagttgtcc caatgaaatc tcaagaattg
     8281 tgttgtttca gctcatttta aataagtagt tttaacaagt aaaaaataac atatttttga
     8341 gtgtgtatat atatatatta caaaatataa taatataaaa tgtaaaagat ataaaccatg
     8401 ctacatatat ttaagagcta ttttgcacat tacttttttg caagccattt tagtgttttt
     8461 tttacaaata tatattagct aaatatacaa tatcttaaat atacaattaa tcagacatgc
     8521 aattaatgca tttaacctac ataaaaatac atgatcaaca tatgttttat gctgattaca
     8581 actaaatcaa acgtaatgaa attaatttat aaaagcgatt taaatatgtc aatattttaa
     8641 gttaaaatca cttgaaacct actataaatg aacttaaatt aaaaaaatct cctttaattc
     8701 caatgcaaca gtgttgtttt ttcagctgag gacccccggt gcagatggtt ggcccccggc
     8761 ccgctattct aaggaagcct ttcttaattt tagggcggcg ctgcatgcgc tcagatcaga
     8821 ttcacgggtg gagagggtta ttttctcttt tttatttttt cttgcctgct catatttggg
     8881 cctgtgtgaa ccccaggctc cgcctcggcc cccaggagcc gctcttattt aaaggggatc
     8941 ctcgccttca gtcttcgtcc cagtggaagc atcagtcttg tgataacaac ttggtgagtc
     9001 ctacagacca gcgctcgact ctaggcttta aatgcattga tttctctgga ttagctaccg
     9061 ggagagtttt aactttgctt attagtcttg tgctcgaact gctaggttta aagagtgttt
     9121 tctctaatgc atgcatgaag cgacagctga aaaccagaaa tagagcgttt gtcctcctgt
     9181 taaactctag cctttctttg gcatttgtca gtccgagtct tcagctgccg gcaggagatt
     9241 gggtttcagt aacacttgct gagcacaaac tacacgtgct ttcttcagat ttaattcatt
     9301 attaatcatt ctagatttaa atgatgttct tactcattgc tgattatttt agccttgcgg
     9361 agcagattaa atgagcacgt gtttcattaa gattgattta aatataatta atatttcacc
     9421 actgtagttt taaaattgcc ttctaagtca tggttgattt ttaataggta tcggctctaa
     9481 ttagattact attttggttt ttaaatgtta tttctatttc atttgtaggc tcgtttttaa
     9541 tgtgatttta ttagattcta gttttaaaat catctcttag ccttaatgag cagactgaat
     9601 gaacatgttt cattaagatt aacttcatta taatgaatta tttaatcatt ctagtgggtt
     9661 ataaatcagc tctatttaaa ttaattgtcg tgtttatttc tatttaattt ttgattattt
     9721 tttcagggtc gtttttatgt gattttttcc cgtttctaat tttaaactaa ttgtttgatt
     9781 tttatatgat tcgactcttt ttaaatgaat tgttttgctt tttttattga gcatatatat
     9841 attatacaat ttcaatcaac aattgttgtg ctttttaaat gttgtttcta tttcatttct
     9901 agggtcgatt ttattcgatt ctagttttaa aatcgtgatt tttaacttct aaccttactg
     9961 agcagactga atgaacatgt ttcatcaaga ttaacttcat tatagttaat tattgaatcg
    10021 tgctagtggt ttataaatca gctctattta aattaatcat catcattatt tctatttaat
    10081 ttttattatt tctttaggat tatttttatg tgattttttt cacgttctag ttttaaactc
    10141 aatgctcatt actgctcacc cttgctgagc agtctttcgt taagattaaa ttaattaata
    10201 accattctag ttttaaaaca gtgtcttaag gcatagctga tattttacaa gtttactatt
    10261 taatatatct cctttctttt tttagggtcg atttcatctg atttgattaa tttttattat
    10321 cttcactaag ctttttgttc taggttttat tctcagtatt attactgtta tatttgaact
    10381 gaatttctcc tcatagttta ttcattttat ttatttattt tttgtttatt cgctattttg
    10441 tattttagtt tttaattaga aattttatta gaatcgtttt gaactagttt ttatgcactc
    10501 taacattaat attaaatcat aactattatt tttattataa tttatttgtg tttatctttt
    10561 tgtgtagctt tcatgtgttt ttattttagt tttattatgt ttgcattgaa ttttttggac
    10621 tagtttatat aacgatatac agaaaagcca tgacaacaaa tgtttttata atacctatta
    10681 agtataagct aatcagattt ccactattct gttcaaagtt aacattatta gccattctga
    10741 tggatcattt gacatttgtc atatgtcttt atgaacactg ctctttcaga tgccactttt
    10801 aaagctgttt tttacagtaa atgaacgttt attattatga cattatttcc taaggcctct
    10861 agagtacagc aggatgtggc gtgtgcatta ccgtgtaatt atgctgactg taaatgtgca
    10921 tttatagact gtcagcatgt ctgactgtgc gctgttgtga ttggtcctgc acagaccaag
//
