LOCUS       sequence-21995-e         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Zuger S, Iwai H
  TITLE     Intein-based biosynthetic incorporation of unlabeled protein tags 
            into isotopically labeled proteins for NMR studies.
  JOURNAL   Nat Biotechnol. 2005 Jun . 23(6):736-40.
  PUBMED    15908942
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2005 Jun . 23(6):736-40."
COMMENT     Sequence Label: pSZBAD1PG
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(375..392)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      545..631
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gggctagaaa taattttgtt taactttaag aaggagatat acatatggtt aaagttatcg
       61 gtcgtcgatc cctgggtgtg caacgcatct ttgatatcgg tctgccgcaa gaccataatt
      121 ttctgctggc taatggtgct atcgccgcca actgttttaa caaaggtacc cagtacaaac
      181 ttatcctgaa cggtaaaacc ctgaaaggtg aaaccaccac cgaagctgtt gacgctgcta
      241 ccgcggaaaa agttttcaaa cagtacgcta acgacaacgg tgttgacggt gaatggacct
      301 acgacgacgc taccaaaacc tacaccgtaa cggaataagc ttggctgttt tggcggatga
      361 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
      421 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
      481 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
      541 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      601 gtcggtgaac gctctcctga gtaagacaaa tccgccggga gcggatttga acgttgcga
//
