LOCUS       sequence-219950-       11913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to 
            sarcoplasmic/endoplasmic calcium ATPase 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11913)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 11913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-SERCA1
FEATURES             Location/Qualifiers
     source          1..11913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1600..1609
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3737..3757)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3765..3781)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3797..3931
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3821..3840)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3875..3894
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(4370..4557,4558..4569)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(4603..4619)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4603..4619)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4656..4676)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4656..4674)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4695..4711)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4708..4730)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4719..4735
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4743..4749,4750..4767,4768..4773))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4788..4809
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4926..4943)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5097..5685)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5177..5196)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5856..6647,6648..6716))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6479..6498
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6717..6821)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6984..7005)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(7354..7365,7366..7528)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     7901..7917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7937..7957
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggctgagaac gcacacacca aggagacacc
      781 agaatgcctg gcctacttcg gcgtgagcga gtccacaggc ctcacaccag aacaggtcaa
      841 gaagaaccaa gccaagtacg gattcaacga gctgccagct gaagaaggta agagcatctg
      901 ggagctggtg gtggagcagt ttgaagatct gttggtcagg attctgcttt tggccgcctg
      961 catctctttt gtactggcct ggtttgagga aggtgaggaa acagtcactg ccttcgtcga
     1021 gcccttcgtt attctcctca tccttatcgc caatgcagtg gttggagtat ggcaggaacg
     1081 caatgcggag agtgccattg aggctctgaa ggaatatgag ccagagatgg gcaaagtcta
     1141 ccgctctgac agaaagagtg tgcagaggat caaggccaga gagattgtcc ctggagacat
     1201 tgtggaagtt tcagttggtg acaaagtccc tgctgatatc agaatcaccg ccatccgctc
     1261 cacaaccctg cgcgtcgacc agtccattct tactggtgag tctgttagtg tgattaaaca
     1321 cacagagtct gttcctgacc ccagggctgt caaccaggac aagaagaaca tgcttttctc
     1381 tggcactaac atcgctgctg gcaaggctat tggagttgtg gttgccactg gtgtgtccac
     1441 tgaaatcggt aaaattcgtg accagatggc tgccactgag caggagaaga cccctctgca
     1501 gcagaagctg gatgagtttg gtgagcaact ctccaaagtc atctctctca tctgtgtggc
     1561 tgtctggctc atcaacatcg gtcacttcaa cgaccccgtc catggtggct cctggatccg
     1621 tggtgccgtc tactacttca agatcgctgt ggctcttgcc gtagctgcca tccccgaagg
     1681 tttgcctgcc gtcatcacaa cctgcttggc tctgggaacc cgccgcatgg ccaagaagaa
     1741 cgccattgtc cgctctcttc cctctgtaga gactctgggc tgcacatctg tcatttgctc
     1801 agacaagact ggcaccctta ccaccaacca gatgtgtgtc acaaagatgt ttgtcattga
     1861 gaaagtggag ggtgaatctg tgactcttga ccagtacgac atttctggat ccaagtacac
     1921 accagaggga gaagttacta agaacggact tcctgtcaag tgcggccaat ttgacggctt
     1981 tgttgagctg gcaaccatct gcgctctgtg caatgactcc tctctggact acaatgagtc
     2041 taagggaatc tatgagaaag ttggcgaggc tacagagact gccctttgct gcctggttga
     2101 aaagatgaat gtgttcaaca ctgatgttcg tggtctgtcc aaggtggaga gagccaacac
     2161 atgttgcgct gtgatcaagc agcttatgaa gaaagacttt actctggagt tctcccgtga
     2221 caggaagtcc atgtctgtct actgctcccc tgcaaaggcc tccaaggctc ctgttggaaa
     2281 caagatgttt gtcaagggtg ctcctgaggg cgttattgac agatgtgcct atgtgcgtgt
     2341 tggcaccact cgtgtgcccc tgactggtcc agttaaagat aagatcatgg ctgtgatcaa
     2401 ggaatgggga actggccgtg atactctgcg ttgcctggcc ctggcaaccc gtgacaaccc
     2461 tctgagacca gaggaaatga acttggaaga ctccaccaag tttgctgaat atgagaccga
     2521 tctcaccttc gtgggttgtg ttggtatgct ggatcctccc cgtaaagagg ttgttggatc
     2581 cattgagctg tgcagggctg ctggcattcg tgtgatcatg atcacaggtg ataacaaggg
     2641 cactgctgtg gctatctgcc gccgtattgg catcttcagt gatgatgagg atgtaacagg
     2701 ccgtgctttc actggccgtg agtttgatga cctgccactg ccccagcaga gagaagcggt
     2761 gcgtaaggcc tgctgttacg ctcgtgttga gccctcccac aagagcaaga tcgttgagtt
     2821 ccttcagggc tttgatgaga ttactgccat gactggtgat ggtgtgaacg atgctcctgc
     2881 cctgaagaag gcagagattg gcattgccat gggctctggc actgctgttg ccaagtcagc
     2941 ctctgagatg gtcctggccg acgacaactt ctctagcatt gtggccgctg ttgaggaggg
     3001 cagagccatt tacaacaaca tgaagcagtt catccgttac ctcatctcct ctaatgttgg
     3061 agaggttgtc tgtattttcc tgactgcagc tcttggtctt cctgaggctc tgatcccagt
     3121 tcagctgctg tgggtgaacc tggtcactga tggtctccct gccactgctc tgggcttcaa
     3181 cccccctgat ttggacatca tgggcaaggc tccccgctcc cccaaagagc ctcttatctc
     3241 cggctggctc ttcttcagat acctggccat tggaggttat gttggtgctg caactgttgc
     3301 tgctgctgga tggtggttcc tctactgtga tgaaggcccc atggtctcct tctaccagct
     3361 gtcccacttc atgcagtgca ctgctgacaa tgaggacttt gccggtattg aatgtgaggt
     3421 gttcgaggcc gctccaccaa tgacaatggc tctgtctgtg ttggtcacca ttgagatgtg
     3481 caatgctctg aacagcttgt cagagaatca gtctcttttg cgtatgcctc catggagcaa
     3541 cttgtggttg gcgggagcca tgactctctc catgtctctg cacttcatga tcatctatgt
     3601 ggaccctctg cctatgatct tcaaactaac ccatctcaac tttgaccagt ggatcgttgt
     3661 actgaagctt tccttcccag tcatcctcat tgacgaagct ctgaaatttg ttgcccgcaa
     3721 ctacctggag gcctagcaac tttattatac atagttgata attcactggc cgtcgtttta
     3781 cggtaccatc gatgatgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     3841 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     3901 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     3961 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     4021 tatggctgat tatgatcctc tagatcagat ctgcgaagat acggccacgg gtgctcttga
     4081 tcctgtggct gattttggac tgtgctgctc gcagctgctg atgaatcaca tacttcctcc
     4141 attttcttcc actgattgac tgttataatt tccctaattt ccaggtcaag gtgctgtgca
     4201 ttgtggtaat agatgtgaca tgacgtcact tccaaaggac caatgaacat gtctgaccaa
     4261 tttcatataa tgtgaaaacg attttcatag gcagaataaa taacatttaa attaaactgg
     4321 gcatcagcgc aattcaattg gtttggtaat agcaagggaa aatagaatga agtgatctcc
     4381 aaaaaataag tactttttga ctgtaaataa aattgtaagg agtaaaaagt actttttttt
     4441 ctaaaaaaat gtaattaagt aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag
     4501 taaaaatccc caaaaataat acttaagtac agtaatcaag taaaattact caagtacttt
     4561 acacctctgg ttcttgaccc cctaccttca gcaagcccag cagatccact agttctagag
     4621 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
     4681 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     4741 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     4801 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     4861 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4921 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4981 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     5041 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5101 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5161 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5221 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5281 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5341 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5401 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5461 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5521 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     5581 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5641 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5701 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaaggcat tttggtcatg
     5761 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5821 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5881 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5941 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6001 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6061 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6121 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6181 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6241 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6301 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6361 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6421 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6481 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6541 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6601 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6661 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6721 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6781 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6841 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     6901 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     6961 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     7021 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
     7081 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
     7141 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
     7201 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
     7261 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
     7321 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
     7381 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
     7441 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
     7501 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
     7561 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
     7621 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
     7681 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
     7741 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
     7801 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     7861 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     7921 gattacgcca agctatcaac tttgtataga aaagttggca cgcgcagatt ccgtgtgggc
     7981 ctggtaatat cttaccattt taagctattt taagattagg gaaaaaaatt aaataaaact
     8041 actttaaaat tactttcctt aaaaagcaac atatttattt agtttttgtt taactggctc
     8101 aagattgtaa tattttactt ttacatgttt ttttaagtac ctaaatgtgt tcaaaagtaa
     8161 agtatgttat tgtttttttt ttttaatgtt agctaagctt taaattgtaa tatattactt
     8221 ttaaaagtga ctttttaaaa atgtaaataa aaaagtaact ataaatgtgt aatgcatgtt
     8281 atttctgtgg gtttttaatt ttcatctcaa ctgggcttac aattttaaca taataaaaat
     8341 caaatcaaat caaatcaggc ccttatttgt cacatacact cttgtatact gaaataaact
     8401 aaatgttgag aaaaatttaa atacaccaaa ataacgcata ccttttttgt aattacctaa
     8461 gtaaaacatt tagttccttt tttaagtgac tcaagattgt aatctattaa ttgtaaaaga
     8521 taaaactagt gatcaatctg acgtgcttct atgcagcctg tgagattaat tgtgatgttt
     8581 aattattaaa caatttaaat ggatcaaaca tttcacatgt gcttattgtg tccaacaaaa
     8641 ccagcagaag cacttaaaaa tattgcatga caacatgagt ttttaatgta aattatatat
     8701 acttcctaaa cgaagttaat catatttcta aaataataat gtcattgaaa tatttaaata
     8761 aatgataagc tttagttgat tcaacataaa attgaaagca ataaggactt ttttttttta
     8821 cagcggtgaa gcaaaagcat gtaataatca tataatataa taaataaata aataaataat
     8881 ggctcttggt gcaaatatga acactttaaa taaatataga ataaatatga tcaataagtc
     8941 ataacgatgt gttttatgtt gattataatt taaacatgtt tctaacgcaa gttttaaaaa
     9001 ctgttttaaa gtattatttt aagttataag atcaatttga ctcaacattt tgtagtgaaa
     9061 atatgttgtg taaaattaat ataatataca ctataaaaat gcagggttcc acacaataca
     9121 ttaatgttgt cccaacacaa atcgattaag ttaacaaata tatgtggatt gaacataaaa
     9181 aattaagttg tcccaatgaa atctcaagaa ttgtgttgtt tcagctcatt ttaaataagt
     9241 agttttaaca agtaaaaaat aacatatttt tgagtgtgta tatatatata ttacaaaata
     9301 taataatata aaatgtaaaa gatataaacc atgctacata tatttaagag ctattttgca
     9361 cattactttt ttgcaagcca ttttagtgtt ttttttacaa atatatatta gctaaatata
     9421 caatatctta aatatacaat taatcagaca tgcaattaat gcatttaacc tacataaaaa
     9481 tacatgatca acatatgttt tatgctgatt acaactaaat caaacgtaat gaaattaatt
     9541 tataaaagcg atttaaatat gtcaatattt taagttaaaa tcacttgaaa cctactataa
     9601 atgaacttaa attaaaaaaa tctcctttaa ttccaatgca acagtgttgt tttttcagct
     9661 gaggaccccc ggtgcagatg gttggccccc ggcccgctat tctaaggaag cctttcttaa
     9721 ttttagggcg gcgctgcatg cgctcagatc agattcacgg gtggagaggg ttattttctc
     9781 ttttttattt tttcttgcct gctcatattt gggcctgtgt gaaccccagg ctccgcctcg
     9841 gcccccagga gccgctctta tttaaagggg atcctcgcct tcagtcttcg tcccagtgga
     9901 agcatcagtc ttgtgataac aacttggtga gtcctacaga ccagcgctcg actctaggct
     9961 ttaaatgcat tgatttctct ggattagcta ccgggagagt tttaactttg cttattagtc
    10021 ttgtgctcga actgctaggt ttaaagagtg ttttctctaa tgcatgcatg aagcgacagc
    10081 tgaaaaccag aaatagagcg tttgtcctcc tgttaaactc tagcctttct ttggcatttg
    10141 tcagtccgag tcttcagctg ccggcaggag attgggtttc agtaacactt gctgagcaca
    10201 aactacacgt gctttcttca gatttaattc attattaatc attctagatt taaatgatgt
    10261 tcttactcat tgctgattat tttagccttg cggagcagat taaatgagca cgtgtttcat
    10321 taagattgat ttaaatataa ttaatatttc accactgtag ttttaaaatt gccttctaag
    10381 tcatggttga tttttaatag gtatcggctc taattagatt actattttgg tttttaaatg
    10441 ttatttctat ttcatttgta ggctcgtttt taatgtgatt ttattagatt ctagttttaa
    10501 aatcatctct tagccttaat gagcagactg aatgaacatg tttcattaag attaacttca
    10561 ttataatgaa ttatttaatc attctagtgg gttataaatc agctctattt aaattaattg
    10621 tcgtgtttat ttctatttaa tttttgatta ttttttcagg gtcgttttta tgtgattttt
    10681 tcccgtttct aattttaaac taattgtttg atttttatat gattcgactc tttttaaatg
    10741 aattgttttg ctttttttat tgagcatata tatattatac aatttcaatc aacaattgtt
    10801 gtgcttttta aatgttgttt ctatttcatt tctagggtcg attttattcg attctagttt
    10861 taaaatcgtg atttttaact tctaacctta ctgagcagac tgaatgaaca tgtttcatca
    10921 agattaactt cattatagtt aattattgaa tcgtgctagt ggtttataaa tcagctctat
    10981 ttaaattaat catcatcatt atttctattt aatttttatt atttctttag gattattttt
    11041 atgtgatttt tttcacgttc tagttttaaa ctcaatgctc attactgctc acccttgctg
    11101 agcagtcttt cgttaagatt aaattaatta ataaccattc tagttttaaa acagtgtctt
    11161 aaggcatagc tgatatttta caagtttact atttaatata tctcctttct ttttttaggg
    11221 tcgatttcat ctgatttgat taatttttat tatcttcact aagctttttg ttctaggttt
    11281 tattctcagt attattactg ttatatttga actgaatttc tcctcatagt ttattcattt
    11341 tatttattta ttttttgttt attcgctatt ttgtatttta gtttttaatt agaaatttta
    11401 ttagaatcgt tttgaactag tttttatgca ctctaacatt aatattaaat cataactatt
    11461 atttttatta taatttattt gtgtttatct ttttgtgtag ctttcatgtg tttttatttt
    11521 agttttatta tgtttgcatt gaattttttg gactagttta tataacgata tacagaaaag
    11581 ccatgacaac aaatgttttt ataataccta ttaagtataa gctaatcaga tttccactat
    11641 tctgttcaaa gttaacatta ttagccattc tgatggatca tttgacattt gtcatatgtc
    11701 tttatgaaca ctgctctttc agatgccact tttaaagctg ttttttacag taaatgaacg
    11761 tttattatta tgacattatt tcctaaggcc tctagagtac agcaggatgt ggcgtgtgca
    11821 ttaccgtgta attatgctga ctgtaaatgt gcatttatag actgtcagca tgtctgactg
    11881 tgcgctgttg tgattggtcc tgcacagacc aag
//
