LOCUS       sequence-21996-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  TITLE     Buchler Lab plasmids
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p404GALL
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     365..388
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     409..425
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     409..425
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(459..475)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(460..476)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(494..512)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 tgcgttcctc gttctttcac cggtcgcgtt tcctgaaacg cagaagttgc ctcgcgccgc
       61 actgctccga acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat
      121 tcggcagtaa cctggcccca caaaccttca aatgaacgaa tcaaattaac aaccatagga
      181 tgataatgcg attagttttt tagccttatt tctggggtaa ttaatcagcg aagcgatgat
      241 ttttgatcta ttaacggata tataaatgca aaaactgcat aaccacttta actaatactt
      301 tcaacatttt cggtttgtat tacttcttat tcaaatgtaa taaaagtatc aacaaaaaat
      361 tgttaatata cctctatact ttaacgtcaa ggagaaaaaa ccccggattc tagaactagt
      421 ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagtca
      481 tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa
      541 aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag
      601 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg
      661 catgtaacat tatactgaaa accttgcttg ag
//
