LOCUS       sequence-219972-       10049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to 2x the PH 
            domain from Human phospholipase C delta 1 (PLCD1, codon optimised). 
            Binds PtdIns(4,5)P2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 10049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-2PH-PLCd-mKate2
FEATURES             Location/Qualifiers
     source          1..10049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(948..972)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             974..1669
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
     polyA_signal    1791..1872
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1873..1893)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1901..1917)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1933..2067
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1957..1976)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(2506..2693,2694..2705)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2739..2755)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2739..2755)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2792..2812)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2792..2810)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2831..2847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2844..2866)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2855..2871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2879..2885,2886..2903,2904..2909))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2924..2945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3062..3079)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3233..3821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3313..3332)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3992..4783,4784..4852))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4615..4634
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4853..4957)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5120..5141)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5490..5501,5502..5664)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6037..6053
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6073..6093
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccgc caccatggac tcgggccggg acttcctgac
       61 cctgcacggc ctacaggatg atgaggatct acaggcgctg ctgaagggca gccagctcct
      121 gaaggtgaag tccagctcat ggaggagaga gcgcttctac aagttgcagg aggactgcaa
      181 gaccatctgg caggagtccc gcaaggtcat gcggaccccg gagtcccagc tgttctccat
      241 cgaggacatt caggaggtgc gaatggggca ccgcacggag ggtctggaga agttcgcccg
      301 tgatgtgccc gaggaccgct gcttctccat tgtcttcaag gaccagcgca atacactaga
      361 cctcatcgcc ccatcgccag ctgatgccca gcactgggtg ctggggctgc acaaaattat
      421 ccatcacagc ggatctatgg accagcgtca gaagctacag cactggattc actcctgctt
      481 gcgaaaagct gacaaaaaca aggacaacaa gataggcggc agcggcggca gcggcggcga
      541 tagcggtaga gattttctga ctctgcatgg actgcaggac gacgaggacc tgcaggccct
      601 gctgaagggc agccagctgc tgaaggtgaa gagcagcagc tggcgccgcg agcgcttcta
      661 caagctgcag gaggactgca agaccatctg gcaggagagc cgcaaggtga tgcgcacccc
      721 cgagagccag ctgttcagca tcgaggacat ccaggaggtg cgcatgggcc accgcaccga
      781 gggcctggag aagttcgccc gcgacgtgcc cgaggaccgc tgcttcagca tcgtgttcaa
      841 ggaccagcgc aacaccctgg acctgatcgc ccccagcccc gccgacgccc agcactgggt
      901 gctgggcctg cacaagatca ttcaccattc tggcagcatg gatgggtacc cagctttctt
      961 gtacaaagtg gctgtgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga
     1021 gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga
     1081 gggcacccag accatgagaa tcaaggcggt cgagggcggc cctctcccct tcgccttcga
     1141 catcctggct accagcttca tgtacggcag caaaaccttc atcaaccaca cccagggcat
     1201 ccccgacttc tttaagcagt ccttccccga gggcttcaca tgggagagag tcaccacata
     1261 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     1321 ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa
     1381 aacactcggc tgggaggcct ccaccgagac cctgtacccc gctgacggcg gcctggaagg
     1441 cagagccgac atggccctga agctcgtggg cgggggccac ctgatctgca acttgaagac
     1501 cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga
     1561 cagaagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt
     1621 ggctgtggcc agatactgcg acctccctag caaactgggg cacagatgag cggccgcgac
     1681 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     1741 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     1801 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     1861 ttttttcact gccaacttta ttatacatag ttgataattc actggccgtc gttttacggt
     1921 accatcgatg atgatccaga catgataaga tacattgatg agtttggaca aaccacaact
     1981 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     2041 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     2101 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     2161 gctgattatg atcctctaga tcagatctgc gaagatacgg ccacgggtgc tcttgatcct
     2221 gtggctgatt ttggactgtg ctgctcgcag ctgctgatga atcacatact tcctccattt
     2281 tcttccactg attgactgtt ataatttccc taatttccag gtcaaggtgc tgtgcattgt
     2341 ggtaatagat gtgacatgac gtcacttcca aaggaccaat gaacatgtct gaccaatttc
     2401 atataatgtg aaaacgattt tcataggcag aataaataac atttaaatta aactgggcat
     2461 cagcgcaatt caattggttt ggtaatagca agggaaaata gaatgaagtg atctccaaaa
     2521 aataagtact ttttgactgt aaataaaatt gtaaggagta aaaagtactt ttttttctaa
     2581 aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa
     2641 aatccccaaa aataatactt aagtacagta atcaagtaaa attactcaag tactttacac
     2701 ctctggttct tgacccccta ccttcagcaa gcccagcaga tccactagtt ctagagcggc
     2761 cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
     2821 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     2881 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     2941 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     3001 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     3061 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     3121 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3181 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3241 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3301 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3361 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3421 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3481 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3541 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3601 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3661 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3721 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3781 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3841 ctacggggtc tgacgctcag tggaacgaaa actcacgtta aggcattttg gtcatgagat
     3901 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3961 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4021 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4081 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     4141 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     4201 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     4261 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     4321 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     4381 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4441 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4501 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4561 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4621 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4681 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4741 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4801 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4861 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4921 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4981 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     5041 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     5101 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     5161 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccacgctggc taagaactca
     5221 tcagcctccc cggtccatct acccacgtac caatgcacca attggccaca atgacggcta
     5281 ctacatggtg ccattccttc ctctttatag gaatggagac tacctcctgt ccaacaatgc
     5341 tcttggatac gagtacgcct acctgttgga cccaggtcat tgcacaacac cagaaatgcc
     5401 ctctgatctg caaaagacgt gaatatctgt tcagacaccc atatccactc tgttccacac
     5461 aggtcagagg tttgtccagg agttcttgac agaggtgtaa aaagtactca aaaattttac
     5521 tcaagtgaaa gtacaagtac ttagggaaaa ttttactcaa ttaaaagtaa aagtatctgg
     5581 ctagaatctt acttgagtaa aagtaaaaaa gtactccatt aaaattgtac ttgagtatta
     5641 aggaagtaaa agtaaaagca agaaagaaaa ctagagattc ttgtttaagc ttttaatctc
     5701 aaaaaacatt aaatgaaatg catacaaggt tttatcctgc tttagaactg tttgtattta
     5761 attatcaaac tataagacag acaatctaat gccagtacac gctactcaaa gttgtaaaac
     5821 ctcagattta acttcagtag aagctgattc tcaaaattgt tagtgtcaag cctagctctt
     5881 ttggggctga aaagcaatcc tgcagtgctg aaaagcctct cacaggcagc cgatgcggga
     5941 agaggtgtat tagtcttgat agagaggctg caaatagcag gaaacgtgag cagagactcc
     6001 ctggtgtctg aaacacaggc cagatgggcc ctcgagcagg aaacagctat gaccatgatt
     6061 acgccaagct atcaactttg tatagaaaag ttggcacgcg cagattccgt gtgggcctgg
     6121 taatatctta ccattttaag ctattttaag attagggaaa aaaattaaat aaaactactt
     6181 taaaattact ttccttaaaa agcaacatat ttatttagtt tttgtttaac tggctcaaga
     6241 ttgtaatatt ttacttttac atgttttttt aagtacctaa atgtgttcaa aagtaaagta
     6301 tgttattgtt tttttttttt aatgttagct aagctttaaa ttgtaatata ttacttttaa
     6361 aagtgacttt ttaaaaatgt aaataaaaaa gtaactataa atgtgtaatg catgttattt
     6421 ctgtgggttt ttaattttca tctcaactgg gcttacaatt ttaacataat aaaaatcaaa
     6481 tcaaatcaaa tcaggccctt atttgtcaca tacactcttg tatactgaaa taaactaaat
     6541 gttgagaaaa atttaaatac accaaaataa cgcatacctt ttttgtaatt acctaagtaa
     6601 aacatttagt tcctttttta agtgactcaa gattgtaatc tattaattgt aaaagataaa
     6661 actagtgatc aatctgacgt gcttctatgc agcctgtgag attaattgtg atgtttaatt
     6721 attaaacaat ttaaatggat caaacatttc acatgtgctt attgtgtcca acaaaaccag
     6781 cagaagcact taaaaatatt gcatgacaac atgagttttt aatgtaaatt atatatactt
     6841 cctaaacgaa gttaatcata tttctaaaat aataatgtca ttgaaatatt taaataaatg
     6901 ataagcttta gttgattcaa cataaaattg aaagcaataa ggactttttt tttttacagc
     6961 ggtgaagcaa aagcatgtaa taatcatata atataataaa taaataaata aataatggct
     7021 cttggtgcaa atatgaacac tttaaataaa tatagaataa atatgatcaa taagtcataa
     7081 cgatgtgttt tatgttgatt ataatttaaa catgtttcta acgcaagttt taaaaactgt
     7141 tttaaagtat tattttaagt tataagatca atttgactca acattttgta gtgaaaatat
     7201 gttgtgtaaa attaatataa tatacactat aaaaatgcag ggttccacac aatacattaa
     7261 tgttgtccca acacaaatcg attaagttaa caaatatatg tggattgaac ataaaaaatt
     7321 aagttgtccc aatgaaatct caagaattgt gttgtttcag ctcattttaa ataagtagtt
     7381 ttaacaagta aaaaataaca tatttttgag tgtgtatata tatatattac aaaatataat
     7441 aatataaaat gtaaaagata taaaccatgc tacatatatt taagagctat tttgcacatt
     7501 acttttttgc aagccatttt agtgtttttt ttacaaatat atattagcta aatatacaat
     7561 atcttaaata tacaattaat cagacatgca attaatgcat ttaacctaca taaaaataca
     7621 tgatcaacat atgttttatg ctgattacaa ctaaatcaaa cgtaatgaaa ttaatttata
     7681 aaagcgattt aaatatgtca atattttaag ttaaaatcac ttgaaaccta ctataaatga
     7741 acttaaatta aaaaaatctc ctttaattcc aatgcaacag tgttgttttt tcagctgagg
     7801 acccccggtg cagatggttg gcccccggcc cgctattcta aggaagcctt tcttaatttt
     7861 agggcggcgc tgcatgcgct cagatcagat tcacgggtgg agagggttat tttctctttt
     7921 ttattttttc ttgcctgctc atatttgggc ctgtgtgaac cccaggctcc gcctcggccc
     7981 ccaggagccg ctcttattta aaggggatcc tcgccttcag tcttcgtccc agtggaagca
     8041 tcagtcttgt gataacaact tggtgagtcc tacagaccag cgctcgactc taggctttaa
     8101 atgcattgat ttctctggat tagctaccgg gagagtttta actttgctta ttagtcttgt
     8161 gctcgaactg ctaggtttaa agagtgtttt ctctaatgca tgcatgaagc gacagctgaa
     8221 aaccagaaat agagcgtttg tcctcctgtt aaactctagc ctttctttgg catttgtcag
     8281 tccgagtctt cagctgccgg caggagattg ggtttcagta acacttgctg agcacaaact
     8341 acacgtgctt tcttcagatt taattcatta ttaatcattc tagatttaaa tgatgttctt
     8401 actcattgct gattatttta gccttgcgga gcagattaaa tgagcacgtg tttcattaag
     8461 attgatttaa atataattaa tatttcacca ctgtagtttt aaaattgcct tctaagtcat
     8521 ggttgatttt taataggtat cggctctaat tagattacta ttttggtttt taaatgttat
     8581 ttctatttca tttgtaggct cgtttttaat gtgattttat tagattctag ttttaaaatc
     8641 atctcttagc cttaatgagc agactgaatg aacatgtttc attaagatta acttcattat
     8701 aatgaattat ttaatcattc tagtgggtta taaatcagct ctatttaaat taattgtcgt
     8761 gtttatttct atttaatttt tgattatttt ttcagggtcg tttttatgtg attttttccc
     8821 gtttctaatt ttaaactaat tgtttgattt ttatatgatt cgactctttt taaatgaatt
     8881 gttttgcttt ttttattgag catatatata ttatacaatt tcaatcaaca attgttgtgc
     8941 tttttaaatg ttgtttctat ttcatttcta gggtcgattt tattcgattc tagttttaaa
     9001 atcgtgattt ttaacttcta accttactga gcagactgaa tgaacatgtt tcatcaagat
     9061 taacttcatt atagttaatt attgaatcgt gctagtggtt tataaatcag ctctatttaa
     9121 attaatcatc atcattattt ctatttaatt tttattattt ctttaggatt atttttatgt
     9181 gatttttttc acgttctagt tttaaactca atgctcatta ctgctcaccc ttgctgagca
     9241 gtctttcgtt aagattaaat taattaataa ccattctagt tttaaaacag tgtcttaagg
     9301 catagctgat attttacaag tttactattt aatatatctc ctttcttttt ttagggtcga
     9361 tttcatctga tttgattaat ttttattatc ttcactaagc tttttgttct aggttttatt
     9421 ctcagtatta ttactgttat atttgaactg aatttctcct catagtttat tcattttatt
     9481 tatttatttt ttgtttattc gctattttgt attttagttt ttaattagaa attttattag
     9541 aatcgttttg aactagtttt tatgcactct aacattaata ttaaatcata actattattt
     9601 ttattataat ttatttgtgt ttatcttttt gtgtagcttt catgtgtttt tattttagtt
     9661 ttattatgtt tgcattgaat tttttggact agtttatata acgatataca gaaaagccat
     9721 gacaacaaat gtttttataa tacctattaa gtataagcta atcagatttc cactattctg
     9781 ttcaaagtta acattattag ccattctgat ggatcatttg acatttgtca tatgtcttta
     9841 tgaacactgc tctttcagat gccactttta aagctgtttt ttacagtaaa tgaacgttta
     9901 ttattatgac attatttcct aaggcctcta gagtacagca ggatgtggcg tgtgcattac
     9961 cgtgtaatta tgctgactgt aaatgtgcat ttatagactg tcagcatgtc tgactgtgcg
    10021 ctgttgtgat tggtcctgca cagaccaag
//
