LOCUS       sequence-219975-        9665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to the PH 
            domain from Human Bruton tyrosine kinase (BTK). Binds 
            PtdIns(3,4,5)P3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-BTK-mKate2
FEATURES             Location/Qualifiers
     source          1..9665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(564..588)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             590..1285
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
     polyA_signal    1407..1488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1489..1509)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1517..1533)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1549..1683
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1573..1592)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(2122..2309,2310..2321)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2355..2371)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2355..2371)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2408..2428)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2408..2426)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2447..2463)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2460..2482)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2471..2487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2495..2501,2502..2519,2520..2525))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2540..2561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2678..2695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2849..3437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2929..2948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3608..4399,4400..4468))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4231..4250
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4469..4573)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4736..4757)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5106..5117,5118..5280)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5653..5669
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5689..5709
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggccgca gtgattctgg agagcatctt
       61 tctgaagcga tcccaacaga aaaagaaaac atcacctcta aacttcaaga agcgcctgtt
      121 tctcttgacc gtgcacaaac tctcctacta tgagtatgac tttgaacgtg ggagaagagg
      181 cagtaagaag ggttcaatag atgttgagaa gatcacttgt gttgaaacag tggttcctga
      241 aaaaaatcct cctccagaaa gacagattcc gagaagaggt gaagagtcca gtgaaatgga
      301 gcaaatttca atcattgaaa ggttccctta tcccttccag gttgtatatg atgaagggcc
      361 tctctacgtc ttctccccaa ctgaagaact aaggaagcgg tggattcacc agctcaaaaa
      421 cgtaatccgg tacaacagtg atctggttca gaaatatcac ccttgcttct ggatcgatgg
      481 gcagtatctc tgctgctctc agacagccaa aaatgctatg ggctgccaaa ttttggagaa
      541 caggaatgga agcttaaaac cggacccagc tttcttgtac aaagtggctg tgagcgagct
      601 gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt
      661 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
      721 ggcggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
      781 cggcagcaaa accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt
      841 ccccgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
      901 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
      961 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac
     1021 cgagaccctg taccccgctg acggcggcct ggaaggcaga gccgacatgg ccctgaagct
     1081 cgtgggcggg ggccacctga tctgcaactt gaagaccaca tacagatcca agaaacccgc
     1141 taagaacctc aagatgcccg gcgtctacta tgtggacaga agactggaaa gaatcaagga
     1201 ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct
     1261 ccctagcaaa ctggggcaca gatgagcggc cgcgactcta gatcataatc agccatacca
     1321 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     1381 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     1441 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcca actttattat
     1501 acatagttga taattcactg gccgtcgttt tacggtacca tcgatgatga tccagacatg
     1561 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     1621 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     1681 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     1741 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagatcag
     1801 atctgcgaag atacggccac gggtgctctt gatcctgtgg ctgattttgg actgtgctgc
     1861 tcgcagctgc tgatgaatca catacttcct ccattttctt ccactgattg actgttataa
     1921 tttccctaat ttccaggtca aggtgctgtg cattgtggta atagatgtga catgacgtca
     1981 cttccaaagg accaatgaac atgtctgacc aatttcatat aatgtgaaaa cgattttcat
     2041 aggcagaata aataacattt aaattaaact gggcatcagc gcaattcaat tggtttggta
     2101 atagcaaggg aaaatagaat gaagtgatct ccaaaaaata agtacttttt gactgtaaat
     2161 aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa
     2221 aagtattgat ttttaattgt actcaagtaa agtaaaaatc cccaaaaata atacttaagt
     2281 acagtaatca agtaaaatta ctcaagtact ttacacctct ggttcttgac cccctacctt
     2341 cagcaagccc agcagatcca ctagttctag agcggccgcc accgcggtgg agctccagct
     2401 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     2461 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     2521 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     2581 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2641 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2701 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2761 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2821 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2881 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2941 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3001 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3061 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3121 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3181 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3241 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3301 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3361 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3421 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3481 acgaaaactc acgttaaggc attttggtca tgagattatc aaaaaggatc ttcacctaga
     3541 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3601 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3661 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3721 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3781 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3841 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3901 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3961 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4021 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4081 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4141 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4201 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4261 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4321 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4381 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4441 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4501 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4561 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     4621 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     4681 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     4741 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     4801 ctatcagggc gatggcccac gctggctaag aactcatcag cctccccggt ccatctaccc
     4861 acgtaccaat gcaccaattg gccacaatga cggctactac atggtgccat tccttcctct
     4921 ttataggaat ggagactacc tcctgtccaa caatgctctt ggatacgagt acgcctacct
     4981 gttggaccca ggtcattgca caacaccaga aatgccctct gatctgcaaa agacgtgaat
     5041 atctgttcag acacccatat ccactctgtt ccacacaggt cagaggtttg tccaggagtt
     5101 cttgacagag gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac aagtacttag
     5161 ggaaaatttt actcaattaa aagtaaaagt atctggctag aatcttactt gagtaaaagt
     5221 aaaaaagtac tccattaaaa ttgtacttga gtattaagga agtaaaagta aaagcaagaa
     5281 agaaaactag agattcttgt ttaagctttt aatctcaaaa aacattaaat gaaatgcata
     5341 caaggtttta tcctgcttta gaactgtttg tatttaatta tcaaactata agacagacaa
     5401 tctaatgcca gtacacgcta ctcaaagttg taaaacctca gatttaactt cagtagaagc
     5461 tgattctcaa aattgttagt gtcaagccta gctcttttgg ggctgaaaag caatcctgca
     5521 gtgctgaaaa gcctctcaca ggcagccgat gcgggaagag gtgtattagt cttgatagag
     5581 aggctgcaaa tagcaggaaa cgtgagcaga gactccctgg tgtctgaaac acaggccaga
     5641 tgggccctcg agcaggaaac agctatgacc atgattacgc caagctatca actttgtata
     5701 gaaaagttgg cacgcgcaga ttccgtgtgg gcctggtaat atcttaccat tttaagctat
     5761 tttaagatta gggaaaaaaa ttaaataaaa ctactttaaa attactttcc ttaaaaagca
     5821 acatatttat ttagtttttg tttaactggc tcaagattgt aatattttac ttttacatgt
     5881 ttttttaagt acctaaatgt gttcaaaagt aaagtatgtt attgtttttt ttttttaatg
     5941 ttagctaagc tttaaattgt aatatattac ttttaaaagt gactttttaa aaatgtaaat
     6001 aaaaaagtaa ctataaatgt gtaatgcatg ttatttctgt gggtttttaa ttttcatctc
     6061 aactgggctt acaattttaa cataataaaa atcaaatcaa atcaaatcag gcccttattt
     6121 gtcacataca ctcttgtata ctgaaataaa ctaaatgttg agaaaaattt aaatacacca
     6181 aaataacgca tacctttttt gtaattacct aagtaaaaca tttagttcct tttttaagtg
     6241 actcaagatt gtaatctatt aattgtaaaa gataaaacta gtgatcaatc tgacgtgctt
     6301 ctatgcagcc tgtgagatta attgtgatgt ttaattatta aacaatttaa atggatcaaa
     6361 catttcacat gtgcttattg tgtccaacaa aaccagcaga agcacttaaa aatattgcat
     6421 gacaacatga gtttttaatg taaattatat atacttccta aacgaagtta atcatatttc
     6481 taaaataata atgtcattga aatatttaaa taaatgataa gctttagttg attcaacata
     6541 aaattgaaag caataaggac tttttttttt tacagcggtg aagcaaaagc atgtaataat
     6601 catataatat aataaataaa taaataaata atggctcttg gtgcaaatat gaacacttta
     6661 aataaatata gaataaatat gatcaataag tcataacgat gtgttttatg ttgattataa
     6721 tttaaacatg tttctaacgc aagttttaaa aactgtttta aagtattatt ttaagttata
     6781 agatcaattt gactcaacat tttgtagtga aaatatgttg tgtaaaatta atataatata
     6841 cactataaaa atgcagggtt ccacacaata cattaatgtt gtcccaacac aaatcgatta
     6901 agttaacaaa tatatgtgga ttgaacataa aaaattaagt tgtcccaatg aaatctcaag
     6961 aattgtgttg tttcagctca ttttaaataa gtagttttaa caagtaaaaa ataacatatt
     7021 tttgagtgtg tatatatata tattacaaaa tataataata taaaatgtaa aagatataaa
     7081 ccatgctaca tatatttaag agctattttg cacattactt ttttgcaagc cattttagtg
     7141 ttttttttac aaatatatat tagctaaata tacaatatct taaatataca attaatcaga
     7201 catgcaatta atgcatttaa cctacataaa aatacatgat caacatatgt tttatgctga
     7261 ttacaactaa atcaaacgta atgaaattaa tttataaaag cgatttaaat atgtcaatat
     7321 tttaagttaa aatcacttga aacctactat aaatgaactt aaattaaaaa aatctccttt
     7381 aattccaatg caacagtgtt gttttttcag ctgaggaccc ccggtgcaga tggttggccc
     7441 ccggcccgct attctaagga agcctttctt aattttaggg cggcgctgca tgcgctcaga
     7501 tcagattcac gggtggagag ggttattttc tcttttttat tttttcttgc ctgctcatat
     7561 ttgggcctgt gtgaacccca ggctccgcct cggcccccag gagccgctct tatttaaagg
     7621 ggatcctcgc cttcagtctt cgtcccagtg gaagcatcag tcttgtgata acaacttggt
     7681 gagtcctaca gaccagcgct cgactctagg ctttaaatgc attgatttct ctggattagc
     7741 taccgggaga gttttaactt tgcttattag tcttgtgctc gaactgctag gtttaaagag
     7801 tgttttctct aatgcatgca tgaagcgaca gctgaaaacc agaaatagag cgtttgtcct
     7861 cctgttaaac tctagccttt ctttggcatt tgtcagtccg agtcttcagc tgccggcagg
     7921 agattgggtt tcagtaacac ttgctgagca caaactacac gtgctttctt cagatttaat
     7981 tcattattaa tcattctaga tttaaatgat gttcttactc attgctgatt attttagcct
     8041 tgcggagcag attaaatgag cacgtgtttc attaagattg atttaaatat aattaatatt
     8101 tcaccactgt agttttaaaa ttgccttcta agtcatggtt gatttttaat aggtatcggc
     8161 tctaattaga ttactatttt ggtttttaaa tgttatttct atttcatttg taggctcgtt
     8221 tttaatgtga ttttattaga ttctagtttt aaaatcatct cttagcctta atgagcagac
     8281 tgaatgaaca tgtttcatta agattaactt cattataatg aattatttaa tcattctagt
     8341 gggttataaa tcagctctat ttaaattaat tgtcgtgttt atttctattt aatttttgat
     8401 tattttttca gggtcgtttt tatgtgattt tttcccgttt ctaattttaa actaattgtt
     8461 tgatttttat atgattcgac tctttttaaa tgaattgttt tgcttttttt attgagcata
     8521 tatatattat acaatttcaa tcaacaattg ttgtgctttt taaatgttgt ttctatttca
     8581 tttctagggt cgattttatt cgattctagt tttaaaatcg tgatttttaa cttctaacct
     8641 tactgagcag actgaatgaa catgtttcat caagattaac ttcattatag ttaattattg
     8701 aatcgtgcta gtggtttata aatcagctct atttaaatta atcatcatca ttatttctat
     8761 ttaattttta ttatttcttt aggattattt ttatgtgatt tttttcacgt tctagtttta
     8821 aactcaatgc tcattactgc tcacccttgc tgagcagtct ttcgttaaga ttaaattaat
     8881 taataaccat tctagtttta aaacagtgtc ttaaggcata gctgatattt tacaagttta
     8941 ctatttaata tatctccttt ctttttttag ggtcgatttc atctgatttg attaattttt
     9001 attatcttca ctaagctttt tgttctaggt tttattctca gtattattac tgttatattt
     9061 gaactgaatt tctcctcata gtttattcat tttatttatt tattttttgt ttattcgcta
     9121 ttttgtattt tagtttttaa ttagaaattt tattagaatc gttttgaact agtttttatg
     9181 cactctaaca ttaatattaa atcataacta ttatttttat tataatttat ttgtgtttat
     9241 ctttttgtgt agctttcatg tgtttttatt ttagttttat tatgtttgca ttgaattttt
     9301 tggactagtt tatataacga tatacagaaa agccatgaca acaaatgttt ttataatacc
     9361 tattaagtat aagctaatca gatttccact attctgttca aagttaacat tattagccat
     9421 tctgatggat catttgacat ttgtcatatg tctttatgaa cactgctctt tcagatgcca
     9481 cttttaaagc tgttttttac agtaaatgaa cgtttattat tatgacatta tttcctaagg
     9541 cctctagagt acagcaggat gtggcgtgtg cattaccgtg taattatgct gactgtaaat
     9601 gtgcatttat agactgtcag catgtctgac tgtgcgctgt tgtgattggt cctgcacaga
     9661 ccaag
//
