LOCUS       sequence-219976-        9721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral delivery of tagRFP-MAD2 expressed from the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Etemad B, Kuijt TE, Kops GJ
  TITLE     Kinetochore-microtubule attachment is sufficient to satisfy the 
            human spindle assembly checkpoint.
  JOURNAL   Nat Commun. 2015 Dec 1;6:8987. doi: 10.1038/ncomms9987.
  PUBMED    26621779
REFERENCE   2  (bases 1 to 9721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-12-1"; volume: "6:8987. doi"; pages: " 
            10.1038/ncomms9987"
COMMENT     Sequence Label: pLV-CMV-tagRFP-Mad2
FEATURES             Location/Qualifiers
     source          1..9721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    251..368
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        391..694
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        695..898
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     849..869
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     895..919
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1060..1770
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    2624..3176
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2768..2785)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2995..3014
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3203..3802
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3203..3222)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3699..3719
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4694..4927
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4999..5120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5036..5055)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5090..5109
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5160..5295
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5315..5334)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5316..5334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5344..5361)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5344..5360)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5353..5375)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5502..5957
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5589..5608)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5799..5820
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5983..6087
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6088..6156,6157..6948)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6306..6325)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7119..7707
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7608..7627
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7861..7878
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7995..8016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8031..8036,8037..8054,8055..8061)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8069..8085
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8074..8096
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8093..8109
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8093..8109
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8128..8148
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8130..8148
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8176..8402
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8403..8583
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8630..8755
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9248..9481
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9666..9710
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
       61 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
      121 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
      181 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
      241 acggtatcgg ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
      301 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
      361 aaaattttat cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc
      421 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      481 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      541 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      601 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      661 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      721 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      781 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      841 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
      901 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
      961 acaccgactc tagctagagg atcccccggg ctgcaggaat tcggcgcgcc acgcgtaccg
     1021 atatccagca cagtggcggc cgctcgagcc tctagagcca tggtgtctaa gggcgaagag
     1081 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac
     1141 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
     1201 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg
     1261 tacggcagca gaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc
     1321 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
     1381 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
     1441 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccaac
     1501 accgagatgc tgtaccccgc tgacggcggc ctggaaggca gaagcgacat ggccctgaag
     1561 ctcgtgggcg ggggccacct gatctgcaac ttcaagacca catacagatc caagaaaccc
     1621 gctaagaacc tcaagatgcc cggcgtctac tatgtggacc acagactgga aagaatcaag
     1681 gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac
     1741 ctccctagca aactggggca caaacttaat gaattctgca gatatccatc acactggcgg
     1801 ccgctcgagc atgcatctag gatggcgctg cagctctccc gggagcaggg aatcaccctg
     1861 cgcgggagcg ccgaaatcgt ggccgagttc ttctcattcg gcatcaacag cattttatat
     1921 cagcgtggca tatatccatc tgaaaccttt actcgagtgc agaaatacgg actcaccttg
     1981 cttgtaacta ctgatcttga gctcataaaa tacctaaata atgtggtgga acaactgaaa
     2041 gattggttat acaagtgttc agttcagaaa ctggttgtag ttatctcaaa tattgaaagt
     2101 ggtgaggtcc tggaaagatg gcagtttgat attgagtgtg acaagactgc aaaagatgac
     2161 agtgcaccca gagaaaagtc tcagaaagct atccaggatg aaatccgttc agtgatcaga
     2221 cagatcacag ctacggtgac atttctgcca ctgttggaag tttcttgttc atttgatctg
     2281 ctgatttata cagacaaaga tttggttgta cctgaaaaat gggaagaatc aggaccacag
     2341 tttattacca attctgagga agtccgcctt cgttcattta ctactacaat ccacaaagta
     2401 aatagcatgg tggcctacaa aattcctgtc aatgactgag ctagcctagt gccatttgtt
     2461 cagtggttcg tagggctttc ccccactgtt tggctttcag ttatatggat gatgtggtat
     2521 tgggggccaa gtctgtacag catcttgagt ccctttttac cgctgttacc aattttcttt
     2581 tgtctttggg taaacccggg aattccgccc ctctcccccc ctaacgttac tggccgaagc
     2641 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     2701 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     2761 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     2821 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     2881 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     2941 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     3001 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     3061 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     3121 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatgga
     3181 tatggccaca acggtcgcca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     3241 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     3301 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     3361 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     3421 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     3481 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     3541 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     3601 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     3661 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     3721 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     3781 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
     3841 cgcacgaccc catgcatgta ttcaatctaa gcaggctttc actttctcgc caacttacaa
     3901 ggcctttctg tgtaaacaat acctgaacct ttaccccgtt gcccggcaac ggccaggtct
     3961 gtgccaagtg tttgctgacg caacccccac tggctggggc ttggtcatgg gccatcagcg
     4021 catgcgtgga accttttcgg ctcctctgcc gatccatact gcggaactcc tagccgcttg
     4081 ttttgctcgc agcaggtctg gagcaaacat tatcgggact gataactctg ttgtcctatc
     4141 ccgcaaatat acatcgtttc catggctgct aggctgtgct gccaactgga tcctgcgcgg
     4201 gacgtccttt gtttacgtcc cgtcggcgct gaatcctgcg gacgaccctt ctcggggtcg
     4261 cttgggactc tctcgtcccc ttctccgtct gccgttccga ccgaccacgg ggcgcacctc
     4321 tctttacgcg gactccccgt ctgtgccttc tcatctgccg gaccgtgtgc acttcgcttc
     4381 acctctgcac gtcgcatgga gaccaccgtg aacgcccacc aaatattgcc caaggtctta
     4441 cataagagga ctcttggact ctcagcaatg tcaacgaccg accttgaggc atacttcaaa
     4501 gactgtttgt ttaaagactg ggaggagttg ggggaggaga ttaggttaaa ggtctttgta
     4561 ctaggaggct gtaggcataa attggtctgc gcaccagcac catggcgcaa tcactagagc
     4621 ggggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     4681 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     4741 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4801 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4861 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4921 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     4981 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5041 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5101 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     5161 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5221 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     5281 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     5341 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5401 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5461 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     5521 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5581 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5641 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5701 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5761 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5821 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     5881 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     5941 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6001 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6061 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6121 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6181 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6241 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6301 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6361 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6421 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6481 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6541 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6601 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6661 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6721 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6781 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6841 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6901 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6961 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7021 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7081 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7141 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7201 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7261 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7321 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7381 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7441 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7501 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7561 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7621 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7681 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7741 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7801 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7861 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7921 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7981 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     8041 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     8101 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     8161 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     8221 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     8281 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     8341 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     8401 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8461 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8521 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8581 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     8641 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     8701 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     8761 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     8821 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     8881 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     8941 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     9001 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     9061 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     9121 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     9181 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     9241 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     9301 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     9361 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     9421 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     9481 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     9541 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     9601 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     9661 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     9721 t
//
