LOCUS       sequence-219981-        9675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to 3xPHD domain
            from Mouse inhibitor of growth family member 2 (ING2), codon 
            optimised. Binds PtdIns5P.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9675)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-ING2
FEATURES             Location/Qualifiers
     source          1..9675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1499..1519)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1527..1543)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1559..1693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1583..1602)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1637..1656
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2132..2319,2320..2331)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2365..2381)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2365..2381)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2418..2438)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2418..2436)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2457..2473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2470..2492)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2481..2497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2505..2511,2512..2529,2530..2535))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2550..2571
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2688..2705)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2859..3447)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2939..2958)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3618..4409,4410..4478))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4241..4260
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4479..4583)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4746..4767)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5116..5127,5128..5290)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5663..5679
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5699..5719
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggcaagt ccagtagaat tcgcaattga
      781 ccccaatgag ccaacttatt gtctttgcaa tcaagttagt tatggcgaga tgataggatg
      841 cgacaacgag cagtgcccaa tagagtggtt ccatttcagt tgcgtatcat tgacatataa
      901 accaaagggc aaatggtatt gcccaaagtg caggggtgac aatgagaaga cgatggacaa
      961 gagtacagag aagacaaaaa aagagcgacg tgctcgtgag ttcgccagtc cggtggagtt
     1021 cgcgattgac ccgaacgagc ccacgtactg cttgtgtaat caggtatcct acggggaaat
     1081 gatcggatgc gacaatgaac agtgtcccat cgagtggttt cacttttcct gcgtgtcttt
     1141 gacatataaa cctaaaggaa aatggtactg cccaaagtgt agaggagata atgaaaagac
     1201 tatggataag tctaccgaga agactaaaaa ggaacgccga gcgagggagt tcgcaagtcc
     1261 cgttgaattt gcgattgacc ctaatgagcc gacttactgc ctttgcaacc aggtttcata
     1321 cggtgagatg atcggctgtg acaacgaaca atgcccaata gaatggttcc acttttcatg
     1381 tgtgagtctt acatataaac ccaaggggaa atggtattgc cccaagtgtc gaggcgacaa
     1441 tgaaaaaacg atggacaaga gcacagaaaa aaccaaaaaa gagagacgtg cgcggtaaca
     1501 actttattat acatagttga taattcactg gccgtcgttt tacggtacca tcgatgatga
     1561 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     1621 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     1681 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     1741 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     1801 tctagatcag atctgcgaag atacggccac gggtgctctt gatcctgtgg ctgattttgg
     1861 actgtgctgc tcgcagctgc tgatgaatca catacttcct ccattttctt ccactgattg
     1921 actgttataa tttccctaat ttccaggtca aggtgctgtg cattgtggta atagatgtga
     1981 catgacgtca cttccaaagg accaatgaac atgtctgacc aatttcatat aatgtgaaaa
     2041 cgattttcat aggcagaata aataacattt aaattaaact gggcatcagc gcaattcaat
     2101 tggtttggta atagcaaggg aaaatagaat gaagtgatct ccaaaaaata agtacttttt
     2161 gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa
     2221 gtaaaagtaa aagtattgat ttttaattgt actcaagtaa agtaaaaatc cccaaaaata
     2281 atacttaagt acagtaatca agtaaaatta ctcaagtact ttacacctct ggttcttgac
     2341 cccctacctt cagcaagccc agcagatcca ctagttctag agcggccgcc accgcggtgg
     2401 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     2461 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     2521 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     2581 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2641 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2701 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2761 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2821 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2881 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2941 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3001 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3061 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3121 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3181 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3241 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3301 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3361 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3421 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3481 gctcagtgga acgaaaactc acgttaaggc attttggtca tgagattatc aaaaaggatc
     3541 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3601 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3661 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3721 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3781 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3841 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3901 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3961 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4021 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4081 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4141 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4201 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4261 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4321 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4381 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4441 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4501 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4561 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     4621 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     4681 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     4741 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     4801 gaaaaaccgt ctatcagggc gatggcccac gctggctaag aactcatcag cctccccggt
     4861 ccatctaccc acgtaccaat gcaccaattg gccacaatga cggctactac atggtgccat
     4921 tccttcctct ttataggaat ggagactacc tcctgtccaa caatgctctt ggatacgagt
     4981 acgcctacct gttggaccca ggtcattgca caacaccaga aatgccctct gatctgcaaa
     5041 agacgtgaat atctgttcag acacccatat ccactctgtt ccacacaggt cagaggtttg
     5101 tccaggagtt cttgacagag gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac
     5161 aagtacttag ggaaaatttt actcaattaa aagtaaaagt atctggctag aatcttactt
     5221 gagtaaaagt aaaaaagtac tccattaaaa ttgtacttga gtattaagga agtaaaagta
     5281 aaagcaagaa agaaaactag agattcttgt ttaagctttt aatctcaaaa aacattaaat
     5341 gaaatgcata caaggtttta tcctgcttta gaactgtttg tatttaatta tcaaactata
     5401 agacagacaa tctaatgcca gtacacgcta ctcaaagttg taaaacctca gatttaactt
     5461 cagtagaagc tgattctcaa aattgttagt gtcaagccta gctcttttgg ggctgaaaag
     5521 caatcctgca gtgctgaaaa gcctctcaca ggcagccgat gcgggaagag gtgtattagt
     5581 cttgatagag aggctgcaaa tagcaggaaa cgtgagcaga gactccctgg tgtctgaaac
     5641 acaggccaga tgggccctcg agcaggaaac agctatgacc atgattacgc caagctatca
     5701 actttgtata gaaaagttgg cacgcgcaga ttccgtgtgg gcctggtaat atcttaccat
     5761 tttaagctat tttaagatta gggaaaaaaa ttaaataaaa ctactttaaa attactttcc
     5821 ttaaaaagca acatatttat ttagtttttg tttaactggc tcaagattgt aatattttac
     5881 ttttacatgt ttttttaagt acctaaatgt gttcaaaagt aaagtatgtt attgtttttt
     5941 ttttttaatg ttagctaagc tttaaattgt aatatattac ttttaaaagt gactttttaa
     6001 aaatgtaaat aaaaaagtaa ctataaatgt gtaatgcatg ttatttctgt gggtttttaa
     6061 ttttcatctc aactgggctt acaattttaa cataataaaa atcaaatcaa atcaaatcag
     6121 gcccttattt gtcacataca ctcttgtata ctgaaataaa ctaaatgttg agaaaaattt
     6181 aaatacacca aaataacgca tacctttttt gtaattacct aagtaaaaca tttagttcct
     6241 tttttaagtg actcaagatt gtaatctatt aattgtaaaa gataaaacta gtgatcaatc
     6301 tgacgtgctt ctatgcagcc tgtgagatta attgtgatgt ttaattatta aacaatttaa
     6361 atggatcaaa catttcacat gtgcttattg tgtccaacaa aaccagcaga agcacttaaa
     6421 aatattgcat gacaacatga gtttttaatg taaattatat atacttccta aacgaagtta
     6481 atcatatttc taaaataata atgtcattga aatatttaaa taaatgataa gctttagttg
     6541 attcaacata aaattgaaag caataaggac tttttttttt tacagcggtg aagcaaaagc
     6601 atgtaataat catataatat aataaataaa taaataaata atggctcttg gtgcaaatat
     6661 gaacacttta aataaatata gaataaatat gatcaataag tcataacgat gtgttttatg
     6721 ttgattataa tttaaacatg tttctaacgc aagttttaaa aactgtttta aagtattatt
     6781 ttaagttata agatcaattt gactcaacat tttgtagtga aaatatgttg tgtaaaatta
     6841 atataatata cactataaaa atgcagggtt ccacacaata cattaatgtt gtcccaacac
     6901 aaatcgatta agttaacaaa tatatgtgga ttgaacataa aaaattaagt tgtcccaatg
     6961 aaatctcaag aattgtgttg tttcagctca ttttaaataa gtagttttaa caagtaaaaa
     7021 ataacatatt tttgagtgtg tatatatata tattacaaaa tataataata taaaatgtaa
     7081 aagatataaa ccatgctaca tatatttaag agctattttg cacattactt ttttgcaagc
     7141 cattttagtg ttttttttac aaatatatat tagctaaata tacaatatct taaatataca
     7201 attaatcaga catgcaatta atgcatttaa cctacataaa aatacatgat caacatatgt
     7261 tttatgctga ttacaactaa atcaaacgta atgaaattaa tttataaaag cgatttaaat
     7321 atgtcaatat tttaagttaa aatcacttga aacctactat aaatgaactt aaattaaaaa
     7381 aatctccttt aattccaatg caacagtgtt gttttttcag ctgaggaccc ccggtgcaga
     7441 tggttggccc ccggcccgct attctaagga agcctttctt aattttaggg cggcgctgca
     7501 tgcgctcaga tcagattcac gggtggagag ggttattttc tcttttttat tttttcttgc
     7561 ctgctcatat ttgggcctgt gtgaacccca ggctccgcct cggcccccag gagccgctct
     7621 tatttaaagg ggatcctcgc cttcagtctt cgtcccagtg gaagcatcag tcttgtgata
     7681 acaacttggt gagtcctaca gaccagcgct cgactctagg ctttaaatgc attgatttct
     7741 ctggattagc taccgggaga gttttaactt tgcttattag tcttgtgctc gaactgctag
     7801 gtttaaagag tgttttctct aatgcatgca tgaagcgaca gctgaaaacc agaaatagag
     7861 cgtttgtcct cctgttaaac tctagccttt ctttggcatt tgtcagtccg agtcttcagc
     7921 tgccggcagg agattgggtt tcagtaacac ttgctgagca caaactacac gtgctttctt
     7981 cagatttaat tcattattaa tcattctaga tttaaatgat gttcttactc attgctgatt
     8041 attttagcct tgcggagcag attaaatgag cacgtgtttc attaagattg atttaaatat
     8101 aattaatatt tcaccactgt agttttaaaa ttgccttcta agtcatggtt gatttttaat
     8161 aggtatcggc tctaattaga ttactatttt ggtttttaaa tgttatttct atttcatttg
     8221 taggctcgtt tttaatgtga ttttattaga ttctagtttt aaaatcatct cttagcctta
     8281 atgagcagac tgaatgaaca tgtttcatta agattaactt cattataatg aattatttaa
     8341 tcattctagt gggttataaa tcagctctat ttaaattaat tgtcgtgttt atttctattt
     8401 aatttttgat tattttttca gggtcgtttt tatgtgattt tttcccgttt ctaattttaa
     8461 actaattgtt tgatttttat atgattcgac tctttttaaa tgaattgttt tgcttttttt
     8521 attgagcata tatatattat acaatttcaa tcaacaattg ttgtgctttt taaatgttgt
     8581 ttctatttca tttctagggt cgattttatt cgattctagt tttaaaatcg tgatttttaa
     8641 cttctaacct tactgagcag actgaatgaa catgtttcat caagattaac ttcattatag
     8701 ttaattattg aatcgtgcta gtggtttata aatcagctct atttaaatta atcatcatca
     8761 ttatttctat ttaattttta ttatttcttt aggattattt ttatgtgatt tttttcacgt
     8821 tctagtttta aactcaatgc tcattactgc tcacccttgc tgagcagtct ttcgttaaga
     8881 ttaaattaat taataaccat tctagtttta aaacagtgtc ttaaggcata gctgatattt
     8941 tacaagttta ctatttaata tatctccttt ctttttttag ggtcgatttc atctgatttg
     9001 attaattttt attatcttca ctaagctttt tgttctaggt tttattctca gtattattac
     9061 tgttatattt gaactgaatt tctcctcata gtttattcat tttatttatt tattttttgt
     9121 ttattcgcta ttttgtattt tagtttttaa ttagaaattt tattagaatc gttttgaact
     9181 agtttttatg cactctaaca ttaatattaa atcataacta ttatttttat tataatttat
     9241 ttgtgtttat ctttttgtgt agctttcatg tgtttttatt ttagttttat tatgtttgca
     9301 ttgaattttt tggactagtt tatataacga tatacagaaa agccatgaca acaaatgttt
     9361 ttataatacc tattaagtat aagctaatca gatttccact attctgttca aagttaacat
     9421 tattagccat tctgatggat catttgacat ttgtcatatg tctttatgaa cactgctctt
     9481 tcagatgcca cttttaaagc tgttttttac agtaaatgaa cgtttattat tatgacatta
     9541 tttcctaagg cctctagagt acagcaggat gtggcgtgtg cattaccgtg taattatgct
     9601 gactgtaaat gtgcatttat agactgtcag catgtctgac tgtgcgctgt tgtgattggt
     9661 cctgcacaga ccaag
//
