LOCUS       sequence-219992-       12349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mifepristone-inducible lentiviral backbone for Gateway cloning of 
            Gene insert together with constitutive mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12349)
  AUTHORS   Bocchi R, Egervari K, Carol-Perdiguer L, Viale B, Quairiaux C, De 
            Roo M, Boitard M, Oskouie S, Salmon P, Kiss JZ
  TITLE     Perturbed Wnt signaling leads to neuronal migration delay, altered 
            interhemispheric connections and impaired social behavior.
  JOURNAL   Nat Commun. 2017 Oct 27;8(1):1158. doi: 10.1038/s41467-017-01046-w.
  PUBMED    29079819
REFERENCE   2  (bases 1 to 12349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-10-27"; volume: "8(1):1158. doi"; pages: " 
            10.1038/s41467-017-01046-w"
COMMENT     Sequence Label: pCLX-G4E1b-R1DESTR2-MIFE-PGK-mCherry
FEATURES             Location/Qualifiers
     source          1..12349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        28..538
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     457..475
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             556..1263
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(698..716)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     966..985
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(1287..1303)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1288..1304)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    1327..1915
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1380..1400)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1798..1809)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2057..2090)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             2110..2290
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2328..2347)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2393..2448
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2447..2466)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2809..2825)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2809..2825)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2822..2844)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2833..2849
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2857..2863,2864..2881,2882..2887))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2902..2923
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2982..3177
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3028..3163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3183..3317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3220..3239)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3274..3293
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3385..3402)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3556..4144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3636..3655)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4315..5106,5107..5175))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4938..4957
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5176..5280)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5311..5690
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             5692..5949
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5996..6121
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6613..6730
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7090..7323
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7508..7552
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     7909..7933
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    8186..8310
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(8335..8340,8341..8358,8359..8365)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             8419..9078
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8485..8504)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             9420..9725
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9604..9623
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(9766..9890)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             11975..12346
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
ORIGIN
        1 ggatcccccg ggctgcagga attccacggg gttggggttg cgccttttcc aaggcagccc
       61 tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga
      121 ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac
      181 ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg
      241 gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga
      301 cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg
      361 ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg
      421 tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc
      481 ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccaggg
      541 ggatggaccg gtgccgtgag caagggcgag gaggataaca tggccatcat caaggagttc
      601 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      661 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      721 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      781 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      841 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      901 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
      961 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1021 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1081 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1141 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1201 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1261 taataggaat tcgatatcaa gcttatcgat accgtcgacc tcgagggggg gcccggtacg
     1321 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     1381 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     1441 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     1501 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     1561 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     1621 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     1681 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     1741 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     1801 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     1861 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     1921 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     1981 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatggga
     2041 tcaattcacc atgggaataa cttcgtatag catacattat acgaagttat gctgcttttt
     2101 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     2161 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     2221 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     2281 atctctagca gcatctagtt gaatcgccag tgtgtcgagc tagcagatct ttttccctct
     2341 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     2401 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     2461 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     2521 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
     2581 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     2641 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     2701 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     2761 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
     2821 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     2881 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2941 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca
     3001 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3061 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3121 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     3181 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3241 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3301 cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3361 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3421 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3481 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3541 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3601 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3661 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3721 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3781 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3841 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3901 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3961 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4021 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4081 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4141 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4201 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4261 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4321 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4381 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4441 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4501 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4561 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4621 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4681 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4741 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4801 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4861 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4921 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4981 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5041 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5101 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5161 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5221 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     5281 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt
     5341 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     5401 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     5461 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     5521 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     5581 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     5641 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ggaggcgtgg
     5701 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     5761 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5821 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5881 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5941 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     6001 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     6061 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     6121 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     6181 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     6241 agttaatact ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     6301 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     6361 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     6421 ggaagagcaa aacaaaagta agaaaaaagc acagcaagca gcaggatctt cagacctgga
     6481 aattccctac aatccccaaa gtcaaggagt agtagaatct atgaataaag aattaaagaa
     6541 aattatagga caggtaagag atcaggctga acatcttaag acagcagtac aaatggcagt
     6601 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     6661 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     6721 tcaaaatttt cgggtttatt acagggacag cagaaatcca ctttggaaag gaccagcaaa
     6781 gctcctctgg aaaggtgaag gggcagtagt aatacaagat aatagtgaca taaaagtagt
     6841 gccaagaaga aaagcaaaga tcattaggga ttatggaaaa cagatggcag gtgatgattg
     6901 tgtggcaagt agacaggatg aggattagaa catggaaaag tttagtaaaa caccataagg
     6961 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     7021 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     7081 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     7141 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     7201 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     7261 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     7321 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     7381 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     7441 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     7501 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     7561 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     7621 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     7681 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccctta cgcgggtcga
     7741 agcggagtac tgtcctccga gtggagtact gtcctccgag cggagtactg tcctccgagt
     7801 cgagggtcga agcggagtac tgtcctccga gtggagtact gtcctccgag cggagtactg
     7861 tcctccgagt cgactctaga gggtatataa tggatctcga gatatcggag ctcgtttagt
     7921 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
     7981 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcgcattc cccgtgttaa
     8041 ttaacaggta agtgtcttcc tcctgtttcc ttcccctgct attctgctca accttcctat
     8101 cagaaactgc agtatctgta tttttgctag cagtaatact aacggttctt tttttctctt
     8161 cacaggccac cactagagtc gaatcacaag tttgtacaaa aaagctgaac gagaaacgta
     8221 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     8281 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca
     8341 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
     8401 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     8461 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     8521 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     8581 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
     8641 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     8701 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     8761 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     8821 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     8881 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     8941 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     9001 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     9061 tggcagggcg gggcgtaaac gcgtggatcc ggcttactaa aagccagata acagtatgcg
     9121 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     9181 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     9241 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     9301 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     9361 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
     9421 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     9481 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     9541 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     9601 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     9661 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     9721 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
     9781 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     9841 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt gattcgacct
     9901 gcaggtcgaa gcggagtact gtcctccgag tttaaaagcg gagtactgtc ctccgaggat
     9961 atcagcggag tactgtcctc cgagtcgcga agcggagtac tgtcctccga gatcgatgtc
    10021 gaccccgccc agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt cggggcggcg
    10081 cggtccgagg tccacttcgc atattaaggt gacgcgtgtg gcctcgaatc gcctggagac
    10141 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc
    10201 gggaacggtg cattggaacg cggattcccc gtgttaatta acaggtaagt gtcttcctcc
    10261 tgtttccttc ccctgctatt ctgctcaacc ttcctatcag aaactgcagt atctgtattt
    10321 ttgctagcag taatactaac ggttcttttt ttctcttcac aggccaccaa gctaccggtc
    10381 caccatggac tcccagcagc cagatctgaa gctactgtct tctatcgaac aagcatgcga
    10441 tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct
    10501 gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc tgactagggc
    10561 acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc tactgatttt
    10621 tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata taaaagcatt
    10681 gttagaattc ccgggtgtcg accagaaaaa gttcaataaa gtcagagttg tgagagcact
    10741 ggatgctgtt gctctcccac agccagtggg cgttccaaat gaaagccaag ccctaagcca
    10801 gagattcact ttttcaccag gtcaagacat acagttgatt ccaccactga tcaacctgtt
    10861 aatgagcatt gaaccagatg tgatctatgc aggacatgac aacacaaaac ctgacacctc
    10921 cagttctttg ctgacaagtc ttaatcaact aggcgagagg caacttcttt cagtagtcaa
    10981 gtggtctaaa tcattgccag gttttcgaaa cttacatatt gatgaccaga taactctcat
    11041 tcagtattct tggatgagct taatggtgtt tggtctagga tggagatcct acaaacacgt
    11101 cagtgggcag atgctgtatt ttgcacctga tctaatacta aatgaacagc ggatgaaaga
    11161 atcatcattc tattcattat gccttaccat gtggcagatc ccacaggagt ttgtcaagct
    11221 tcaagttagc caagaagagt tcctctgtat gaaagtattg ttacttctta atacaattcc
    11281 tttggaaggg ctacgaagtc aaacccagtt tgaggagatg aggtcaagct acattagaga
    11341 gctcatcaag gcaattggtt tgaggcaaaa aggagttgtg tcgagctcac agcgtttcta
    11401 tcaacttaca aaacttcttg ataacttgca tgatcttgtc aaacaacttc atctgtactg
    11461 cttgaataca tttatccagt cccgggcact gagtgttgaa tttccagaaa tgatgtctga
    11521 agttattgct gggtcgacgc ccatggaatt ccagtacctg ccagatacag acgatcgtca
    11581 ccggattgag gagaaacgta aaaggacata tgagaccttc aagagcatca tgaagaagag
    11641 tcctttcagc ggacccaccg acccccggcc tccacctcga cgcattgctg tgccttcccg
    11701 cagctcagct tctgtcccca agccagcacc ccagccctat ccctttacgt catccctgag
    11761 caccatcaac tatgatgagt ttcccaccat ggtgtttcct tctgggcaga tcagccaggc
    11821 ctcggccttg gccccggccc ctccccaagt cctgccccag gctccagccc ctgcccctgc
    11881 tccagccatg gtatcagctc tggcccaggc cccagcccct gtcccagtcc tagccccagg
    11941 ccctcctcag gctgtggccc cacctgcccc caagcccacc caggctgggg aaggaacgct
    12001 gtcagaggcc ctgctgcagc tgcagtttga tgatgaagac ctgggggcct tgcttggcaa
    12061 cagcacagac ccagctgtgt tcacagacct ggcatccgtc gacaactccg agtttcagca
    12121 gctgctgaac cagggcatac ctgtggcccc ccacacaact gagcccatgc tgatggagta
    12181 ccctgaggct ataactcgcc tagtgacagg ggcccagagg ccccccgacc cagctcctgc
    12241 tccactgggg gccccggggc tccccaatgg cctcctttca ggagatgaag acttctcctc
    12301 cattgcggac atggacttct cagccctgct gagtcagatc agctcctaa
//
