LOCUS       sequence-219995-       10431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to yeast ATG18 
            (PLEKHA3). Binds PtdIns(3,5P)2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 10431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-ATG18
FEATURES             Location/Qualifiers
     source          1..10431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2255..2275)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2283..2299)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2315..2449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2339..2358)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2393..2412
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2888..3075,3076..3087)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3121..3137)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3121..3137)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3174..3194)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3174..3192)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3213..3229)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3226..3248)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3237..3253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3261..3267,3268..3285,3286..3291))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3306..3327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3444..3461)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3615..4203)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3695..3714)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4374..5165,5166..5234))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4997..5016
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5235..5339)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5502..5523)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5872..5883,5884..6046)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6419..6435
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6455..6475
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtgtctgat tcatcaccta ctatcaactt
      781 tattaatttc aatcaaaccg gaacgtgtat ttcccttgga acgtcgaaag gtttcaaaat
      841 attcaattgt gagcccttcg gaaaatttta ttcagaggac agtgggggct atgctatcgt
      901 cgagatgttg ttctccacct cgttactagc cctcgttggg ataggcgatc aacctgcgct
      961 ttcaccaagg agattgcgta taatcaacac aaaaaaacat tctattatct gtgaggtgac
     1021 tttccctact tctattctga gtgtgaaaat gaataagtct cgattggtgg tacttttaca
     1081 agagcagatt tatatttatg atatcaacac catgagacta ttgcatacta tagaaacaaa
     1141 ccctaaccca cgtggcctta tggctatgtc tccttcggta gccaacagct atttagtgta
     1201 tccatcacca ccaaaagtta ttaactccga aattaaagct catgccacca caaacaatat
     1261 cacattgtca gttggtggca acacagagac cagtttcaag agagatcagc aagatgctgg
     1321 ccatagtgac attagcgact tggatcagta ttcgagcttt actaagaggg atgatgcgga
     1381 tccaacaagc agtaacggcg gtaacagcag tataataaag aatggtgatg tgatcgtatt
     1441 caacttggaa acattacagc caaccatggt catcgaagct cataagggcg agattgctgc
     1501 aatggcaatt agttttgatg ggacactaat ggctaccgcc tctgataaag gtactatcat
     1561 cagggtcttt gacattgaaa cgggtgataa gatctaccaa ttcaggagag ggacgtacgc
     1621 gacaagaatt tactccatat cattcagtga agatagccag tacttggcgg ttaccggctc
     1681 ttccaaaacc gtgcatatct tcaaattggg gcattcaatg agcaacaata aactagacag
     1741 cgatgatagc aacatggaag aagctgcagc cgatgattca tcgctcgata ccaccagtat
     1801 cgatgcgctg agtgacgagg aaaacccgac aagactcgca agagaaccat atgtggatgc
     1861 atcaagaaag acaatgggta ggatgatacg ttactcttct caaaagctat cccgaagagc
     1921 tgccagaaca ttgggtcaga ttttccccat caaagttaca tcgttgttgg aatcctcgcg
     1981 ccattttgcg tctttgaaac ttcccgttga aaccaattcc catgtaatga ccatatcaag
     2041 tataggctct ccaatagata tagacacatc cgagtatccg gaactcttcg aaactggcaa
     2101 ttccgcaagt acagagtcct accatgagcc tgttatgaag atggtcccca tcagggtcgt
     2161 ttcctcggat ggatacctat acaactttgt tatggacccg gagagaggcg gcgattgctt
     2221 aatattgtca cagtattcca tcttgatgga ttgacaactt tattatacat agttgataat
     2281 tcactggccg tcgttttacg gtaccatcga tgatgatcca gacatgataa gatacattga
     2341 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     2401 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     2461 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     2521 aaacctctac aaatgtggta tggctgatta tgatcctcta gatcagatct gcgaagatac
     2581 ggccacgggt gctcttgatc ctgtggctga ttttggactg tgctgctcgc agctgctgat
     2641 gaatcacata cttcctccat tttcttccac tgattgactg ttataatttc cctaatttcc
     2701 aggtcaaggt gctgtgcatt gtggtaatag atgtgacatg acgtcacttc caaaggacca
     2761 atgaacatgt ctgaccaatt tcatataatg tgaaaacgat tttcataggc agaataaata
     2821 acatttaaat taaactgggc atcagcgcaa ttcaattggt ttggtaatag caagggaaaa
     2881 tagaatgaag tgatctccaa aaaataagta ctttttgact gtaaataaaa ttgtaaggag
     2941 taaaaagtac ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt attgattttt
     3001 aattgtactc aagtaaagta aaaatcccca aaaataatac ttaagtacag taatcaagta
     3061 aaattactca agtactttac acctctggtt cttgaccccc taccttcagc aagcccagca
     3121 gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag
     3181 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3241 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3301 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3361 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3421 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3481 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3541 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3601 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3661 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3721 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3781 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3841 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3901 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3961 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4021 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4081 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4141 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4201 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4261 taaggcattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4321 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4381 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4441 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4501 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4561 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4621 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4681 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4741 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     4801 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4861 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4921 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4981 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5041 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5101 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5161 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5221 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5281 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5341 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     5401 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     5461 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     5521 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     5581 gcccacgctg gctaagaact catcagcctc cccggtccat ctacccacgt accaatgcac
     5641 caattggcca caatgacggc tactacatgg tgccattcct tcctctttat aggaatggag
     5701 actacctcct gtccaacaat gctcttggat acgagtacgc ctacctgttg gacccaggtc
     5761 attgcacaac accagaaatg ccctctgatc tgcaaaagac gtgaatatct gttcagacac
     5821 ccatatccac tctgttccac acaggtcaga ggtttgtcca ggagttcttg acagaggtgt
     5881 aaaaagtact caaaaatttt actcaagtga aagtacaagt acttagggaa aattttactc
     5941 aattaaaagt aaaagtatct ggctagaatc ttacttgagt aaaagtaaaa aagtactcca
     6001 ttaaaattgt acttgagtat taaggaagta aaagtaaaag caagaaagaa aactagagat
     6061 tcttgtttaa gcttttaatc tcaaaaaaca ttaaatgaaa tgcatacaag gttttatcct
     6121 gctttagaac tgtttgtatt taattatcaa actataagac agacaatcta atgccagtac
     6181 acgctactca aagttgtaaa acctcagatt taacttcagt agaagctgat tctcaaaatt
     6241 gttagtgtca agcctagctc ttttggggct gaaaagcaat cctgcagtgc tgaaaagcct
     6301 ctcacaggca gccgatgcgg gaagaggtgt attagtcttg atagagaggc tgcaaatagc
     6361 aggaaacgtg agcagagact ccctggtgtc tgaaacacag gccagatggg ccctcgagca
     6421 ggaaacagct atgaccatga ttacgccaag ctatcaactt tgtatagaaa agttggcacg
     6481 cgcagattcc gtgtgggcct ggtaatatct taccatttta agctatttta agattaggga
     6541 aaaaaattaa ataaaactac tttaaaatta ctttccttaa aaagcaacat atttatttag
     6601 tttttgttta actggctcaa gattgtaata ttttactttt acatgttttt ttaagtacct
     6661 aaatgtgttc aaaagtaaag tatgttattg tttttttttt ttaatgttag ctaagcttta
     6721 aattgtaata tattactttt aaaagtgact ttttaaaaat gtaaataaaa aagtaactat
     6781 aaatgtgtaa tgcatgttat ttctgtgggt ttttaatttt catctcaact gggcttacaa
     6841 ttttaacata ataaaaatca aatcaaatca aatcaggccc ttatttgtca catacactct
     6901 tgtatactga aataaactaa atgttgagaa aaatttaaat acaccaaaat aacgcatacc
     6961 ttttttgtaa ttacctaagt aaaacattta gttccttttt taagtgactc aagattgtaa
     7021 tctattaatt gtaaaagata aaactagtga tcaatctgac gtgcttctat gcagcctgtg
     7081 agattaattg tgatgtttaa ttattaaaca atttaaatgg atcaaacatt tcacatgtgc
     7141 ttattgtgtc caacaaaacc agcagaagca cttaaaaata ttgcatgaca acatgagttt
     7201 ttaatgtaaa ttatatatac ttcctaaacg aagttaatca tatttctaaa ataataatgt
     7261 cattgaaata tttaaataaa tgataagctt tagttgattc aacataaaat tgaaagcaat
     7321 aaggactttt tttttttaca gcggtgaagc aaaagcatgt aataatcata taatataata
     7381 aataaataaa taaataatgg ctcttggtgc aaatatgaac actttaaata aatatagaat
     7441 aaatatgatc aataagtcat aacgatgtgt tttatgttga ttataattta aacatgtttc
     7501 taacgcaagt tttaaaaact gttttaaagt attattttaa gttataagat caatttgact
     7561 caacattttg tagtgaaaat atgttgtgta aaattaatat aatatacact ataaaaatgc
     7621 agggttccac acaatacatt aatgttgtcc caacacaaat cgattaagtt aacaaatata
     7681 tgtggattga acataaaaaa ttaagttgtc ccaatgaaat ctcaagaatt gtgttgtttc
     7741 agctcatttt aaataagtag ttttaacaag taaaaaataa catatttttg agtgtgtata
     7801 tatatatatt acaaaatata ataatataaa atgtaaaaga tataaaccat gctacatata
     7861 tttaagagct attttgcaca ttactttttt gcaagccatt ttagtgtttt ttttacaaat
     7921 atatattagc taaatataca atatcttaaa tatacaatta atcagacatg caattaatgc
     7981 atttaaccta cataaaaata catgatcaac atatgtttta tgctgattac aactaaatca
     8041 aacgtaatga aattaattta taaaagcgat ttaaatatgt caatatttta agttaaaatc
     8101 acttgaaacc tactataaat gaacttaaat taaaaaaatc tcctttaatt ccaatgcaac
     8161 agtgttgttt tttcagctga ggacccccgg tgcagatggt tggcccccgg cccgctattc
     8221 taaggaagcc tttcttaatt ttagggcggc gctgcatgcg ctcagatcag attcacgggt
     8281 ggagagggtt attttctctt ttttattttt tcttgcctgc tcatatttgg gcctgtgtga
     8341 accccaggct ccgcctcggc ccccaggagc cgctcttatt taaaggggat cctcgccttc
     8401 agtcttcgtc ccagtggaag catcagtctt gtgataacaa cttggtgagt cctacagacc
     8461 agcgctcgac tctaggcttt aaatgcattg atttctctgg attagctacc gggagagttt
     8521 taactttgct tattagtctt gtgctcgaac tgctaggttt aaagagtgtt ttctctaatg
     8581 catgcatgaa gcgacagctg aaaaccagaa atagagcgtt tgtcctcctg ttaaactcta
     8641 gcctttcttt ggcatttgtc agtccgagtc ttcagctgcc ggcaggagat tgggtttcag
     8701 taacacttgc tgagcacaaa ctacacgtgc tttcttcaga tttaattcat tattaatcat
     8761 tctagattta aatgatgttc ttactcattg ctgattattt tagccttgcg gagcagatta
     8821 aatgagcacg tgtttcatta agattgattt aaatataatt aatatttcac cactgtagtt
     8881 ttaaaattgc cttctaagtc atggttgatt tttaataggt atcggctcta attagattac
     8941 tattttggtt tttaaatgtt atttctattt catttgtagg ctcgttttta atgtgatttt
     9001 attagattct agttttaaaa tcatctctta gccttaatga gcagactgaa tgaacatgtt
     9061 tcattaagat taacttcatt ataatgaatt atttaatcat tctagtgggt tataaatcag
     9121 ctctatttaa attaattgtc gtgtttattt ctatttaatt tttgattatt ttttcagggt
     9181 cgtttttatg tgattttttc ccgtttctaa ttttaaacta attgtttgat ttttatatga
     9241 ttcgactctt tttaaatgaa ttgttttgct ttttttattg agcatatata tattatacaa
     9301 tttcaatcaa caattgttgt gctttttaaa tgttgtttct atttcatttc tagggtcgat
     9361 tttattcgat tctagtttta aaatcgtgat ttttaacttc taaccttact gagcagactg
     9421 aatgaacatg tttcatcaag attaacttca ttatagttaa ttattgaatc gtgctagtgg
     9481 tttataaatc agctctattt aaattaatca tcatcattat ttctatttaa tttttattat
     9541 ttctttagga ttatttttat gtgatttttt tcacgttcta gttttaaact caatgctcat
     9601 tactgctcac ccttgctgag cagtctttcg ttaagattaa attaattaat aaccattcta
     9661 gttttaaaac agtgtcttaa ggcatagctg atattttaca agtttactat ttaatatatc
     9721 tcctttcttt ttttagggtc gatttcatct gatttgatta atttttatta tcttcactaa
     9781 gctttttgtt ctaggtttta ttctcagtat tattactgtt atatttgaac tgaatttctc
     9841 ctcatagttt attcatttta tttatttatt ttttgtttat tcgctatttt gtattttagt
     9901 ttttaattag aaattttatt agaatcgttt tgaactagtt tttatgcact ctaacattaa
     9961 tattaaatca taactattat ttttattata atttatttgt gtttatcttt ttgtgtagct
    10021 ttcatgtgtt tttattttag ttttattatg tttgcattga attttttgga ctagtttata
    10081 taacgatata cagaaaagcc atgacaacaa atgtttttat aatacctatt aagtataagc
    10141 taatcagatt tccactattc tgttcaaagt taacattatt agccattctg atggatcatt
    10201 tgacatttgt catatgtctt tatgaacact gctctttcag atgccacttt taaagctgtt
    10261 ttttacagta aatgaacgtt tattattatg acattatttc ctaaggcctc tagagtacag
    10321 caggatgtgg cgtgtgcatt accgtgtaat tatgctgact gtaaatgtgc atttatagac
    10381 tgtcagcatg tctgactgtg cgctgttgtg attggtcctg cacagaccaa g
//
