LOCUS       sequence-220017-        9896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to lamp1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-lamp1-mKate2
FEATURES             Location/Qualifiers
     source          1..9896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(792..816)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             818..1516
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    1638..1719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1720..1740)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1748..1764)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1780..1914
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1804..1823)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(2353..2540,2541..2552)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2586..2602)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2586..2602)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2639..2659)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2639..2657)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2678..2694)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2691..2713)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2702..2718
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2726..2732,2733..2750,2751..2756))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2771..2792
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2909..2926)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3080..3668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3160..3179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3839..4630,4631..4699))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4462..4481
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4700..4804)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4967..4988)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5337..5348,5349..5511)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5884..5900
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5920..5940
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catgatctct atcatcccca aacaaaacag
       61 acccgtcggg gcaattttta gcctgctgtt agctctaacc atacaccaga ctttgacctc
      121 caatgttccg accactgcgg ccacccctga aaccagctct cctccggcct ctcctgtcac
      181 ccccggaccc ccagagcggg gcaactacaa tgtcaccaat gccaatgata cagtctgtct
      241 cctggcacgc atggccctcc aactcaacat aagcttcaac tcaacctctc agggcaagct
      301 tgtccaggag gtcttgaact tgcatccaaa cctcgttaaa ttctctggat cctgtgagtt
      361 ggaccgtgca tctctgattc tcacagagga caacattact ctgactttca gcttctcttt
      421 aaactccaca tcaaacaagt atcatcttag tggtttggag ctgtcagctt cattgcctga
      481 tatggctaag ggtcttgtgg tcagaaacac cacactgaac tactttgtgg gaacgctggg
      541 ccactcttac atgtgtcaga aggaaaagac cttgagcgtc acccaaaatt tctctctcaa
      601 caccttccaa cttcaagtgc aacctttcgg tgtcaaggga gattttggag cagctgagga
      661 gtgcgagttg gatgaggacg acatgttgat ccccatcgtt gtgggcgctg cattggctgt
      721 tctagtgctc atagtgatcc tggcgtacct gatcggcaga aacaggagtc acgccggcta
      781 ccagaccatc cacccagctt tcttgtacaa agtggctatg gtgagcgagc tgattaagga
      841 gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac
      901 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggcggtcga
      961 gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcaa
     1021 aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct tccccgaggg
     1081 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
     1141 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc
     1201 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggcctcca ccgagaccct
     1261 gtaccccgct gacggcggcc tggaaggcag agccgacatg gccctgaagc tcgtgggcgg
     1321 gggccacctg atctgcaact tgaagaccac atacagatcc aagaaacccg ctaagaacct
     1381 caagatgccc ggcgtctact atgtggacag aagactggaa agaatcaagg aggccgacaa
     1441 agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa
     1501 actggggcac agatgagcgg ccgcgactct agatcataat cagccatacc acatttgtag
     1561 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     1621 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     1681 gcatcacaaa tttcacaaat aaagcatttt tttcactgcc aactttatta tacatagttg
     1741 ataattcact ggccgtcgtt ttacggtacc atcgatgatg atccagacat gataagatac
     1801 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     1861 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     1921 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     1981 aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagatca gatctgcgaa
     2041 gatacggcca cgggtgctct tgatcctgtg gctgattttg gactgtgctg ctcgcagctg
     2101 ctgatgaatc acatacttcc tccattttct tccactgatt gactgttata atttccctaa
     2161 tttccaggtc aaggtgctgt gcattgtggt aatagatgtg acatgacgtc acttccaaag
     2221 gaccaatgaa catgtctgac caatttcata taatgtgaaa acgattttca taggcagaat
     2281 aaataacatt taaattaaac tgggcatcag cgcaattcaa ttggtttggt aatagcaagg
     2341 gaaaatagaa tgaagtgatc tccaaaaaat aagtactttt tgactgtaaa taaaattgta
     2401 aggagtaaaa agtacttttt tttctaaaaa aatgtaatta agtaaaagta aaagtattga
     2461 tttttaattg tactcaagta aagtaaaaat ccccaaaaat aatacttaag tacagtaatc
     2521 aagtaaaatt actcaagtac tttacacctc tggttcttga ccccctacct tcagcaagcc
     2581 cagcagatcc actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc
     2641 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     2701 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     2761 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     2821 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     2881 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2941 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3001 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3061 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3121 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3181 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3241 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3301 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3361 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3421 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3481 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3541 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3601 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3661 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3721 cacgttaagg cattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3781 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3841 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3901 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3961 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4021 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4081 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4141 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4201 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4261 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4321 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4381 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4441 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4501 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4561 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4621 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4681 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4741 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4801 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
     4861 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     4921 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     4981 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     5041 cgatggccca cgctggctaa gaactcatca gcctccccgg tccatctacc cacgtaccaa
     5101 tgcaccaatt ggccacaatg acggctacta catggtgcca ttccttcctc tttataggaa
     5161 tggagactac ctcctgtcca acaatgctct tggatacgag tacgcctacc tgttggaccc
     5221 aggtcattgc acaacaccag aaatgccctc tgatctgcaa aagacgtgaa tatctgttca
     5281 gacacccata tccactctgt tccacacagg tcagaggttt gtccaggagt tcttgacaga
     5341 ggtgtaaaaa gtactcaaaa attttactca agtgaaagta caagtactta gggaaaattt
     5401 tactcaatta aaagtaaaag tatctggcta gaatcttact tgagtaaaag taaaaaagta
     5461 ctccattaaa attgtacttg agtattaagg aagtaaaagt aaaagcaaga aagaaaacta
     5521 gagattcttg tttaagcttt taatctcaaa aaacattaaa tgaaatgcat acaaggtttt
     5581 atcctgcttt agaactgttt gtatttaatt atcaaactat aagacagaca atctaatgcc
     5641 agtacacgct actcaaagtt gtaaaacctc agatttaact tcagtagaag ctgattctca
     5701 aaattgttag tgtcaagcct agctcttttg gggctgaaaa gcaatcctgc agtgctgaaa
     5761 agcctctcac aggcagccga tgcgggaaga ggtgtattag tcttgataga gaggctgcaa
     5821 atagcaggaa acgtgagcag agactccctg gtgtctgaaa cacaggccag atgggccctc
     5881 gagcaggaaa cagctatgac catgattacg ccaagctatc aactttgtat agaaaagttg
     5941 gcacgcgcag attccgtgtg ggcctggtaa tatcttacca ttttaagcta ttttaagatt
     6001 agggaaaaaa attaaataaa actactttaa aattactttc cttaaaaagc aacatattta
     6061 tttagttttt gtttaactgg ctcaagattg taatatttta cttttacatg tttttttaag
     6121 tacctaaatg tgttcaaaag taaagtatgt tattgttttt tttttttaat gttagctaag
     6181 ctttaaattg taatatatta cttttaaaag tgacttttta aaaatgtaaa taaaaaagta
     6241 actataaatg tgtaatgcat gttatttctg tgggttttta attttcatct caactgggct
     6301 tacaatttta acataataaa aatcaaatca aatcaaatca ggcccttatt tgtcacatac
     6361 actcttgtat actgaaataa actaaatgtt gagaaaaatt taaatacacc aaaataacgc
     6421 ataccttttt tgtaattacc taagtaaaac atttagttcc ttttttaagt gactcaagat
     6481 tgtaatctat taattgtaaa agataaaact agtgatcaat ctgacgtgct tctatgcagc
     6541 ctgtgagatt aattgtgatg tttaattatt aaacaattta aatggatcaa acatttcaca
     6601 tgtgcttatt gtgtccaaca aaaccagcag aagcacttaa aaatattgca tgacaacatg
     6661 agtttttaat gtaaattata tatacttcct aaacgaagtt aatcatattt ctaaaataat
     6721 aatgtcattg aaatatttaa ataaatgata agctttagtt gattcaacat aaaattgaaa
     6781 gcaataagga cttttttttt ttacagcggt gaagcaaaag catgtaataa tcatataata
     6841 taataaataa ataaataaat aatggctctt ggtgcaaata tgaacacttt aaataaatat
     6901 agaataaata tgatcaataa gtcataacga tgtgttttat gttgattata atttaaacat
     6961 gtttctaacg caagttttaa aaactgtttt aaagtattat tttaagttat aagatcaatt
     7021 tgactcaaca ttttgtagtg aaaatatgtt gtgtaaaatt aatataatat acactataaa
     7081 aatgcagggt tccacacaat acattaatgt tgtcccaaca caaatcgatt aagttaacaa
     7141 atatatgtgg attgaacata aaaaattaag ttgtcccaat gaaatctcaa gaattgtgtt
     7201 gtttcagctc attttaaata agtagtttta acaagtaaaa aataacatat ttttgagtgt
     7261 gtatatatat atattacaaa atataataat ataaaatgta aaagatataa accatgctac
     7321 atatatttaa gagctatttt gcacattact tttttgcaag ccattttagt gtttttttta
     7381 caaatatata ttagctaaat atacaatatc ttaaatatac aattaatcag acatgcaatt
     7441 aatgcattta acctacataa aaatacatga tcaacatatg ttttatgctg attacaacta
     7501 aatcaaacgt aatgaaatta atttataaaa gcgatttaaa tatgtcaata ttttaagtta
     7561 aaatcacttg aaacctacta taaatgaact taaattaaaa aaatctcctt taattccaat
     7621 gcaacagtgt tgttttttca gctgaggacc cccggtgcag atggttggcc cccggcccgc
     7681 tattctaagg aagcctttct taattttagg gcggcgctgc atgcgctcag atcagattca
     7741 cgggtggaga gggttatttt ctctttttta ttttttcttg cctgctcata tttgggcctg
     7801 tgtgaacccc aggctccgcc tcggccccca ggagccgctc ttatttaaag gggatcctcg
     7861 ccttcagtct tcgtcccagt ggaagcatca gtcttgtgat aacaacttgg tgagtcctac
     7921 agaccagcgc tcgactctag gctttaaatg cattgatttc tctggattag ctaccgggag
     7981 agttttaact ttgcttatta gtcttgtgct cgaactgcta ggtttaaaga gtgttttctc
     8041 taatgcatgc atgaagcgac agctgaaaac cagaaataga gcgtttgtcc tcctgttaaa
     8101 ctctagcctt tctttggcat ttgtcagtcc gagtcttcag ctgccggcag gagattgggt
     8161 ttcagtaaca cttgctgagc acaaactaca cgtgctttct tcagatttaa ttcattatta
     8221 atcattctag atttaaatga tgttcttact cattgctgat tattttagcc ttgcggagca
     8281 gattaaatga gcacgtgttt cattaagatt gatttaaata taattaatat ttcaccactg
     8341 tagttttaaa attgccttct aagtcatggt tgatttttaa taggtatcgg ctctaattag
     8401 attactattt tggtttttaa atgttatttc tatttcattt gtaggctcgt ttttaatgtg
     8461 attttattag attctagttt taaaatcatc tcttagcctt aatgagcaga ctgaatgaac
     8521 atgtttcatt aagattaact tcattataat gaattattta atcattctag tgggttataa
     8581 atcagctcta tttaaattaa ttgtcgtgtt tatttctatt taatttttga ttattttttc
     8641 agggtcgttt ttatgtgatt ttttcccgtt tctaatttta aactaattgt ttgattttta
     8701 tatgattcga ctctttttaa atgaattgtt ttgctttttt tattgagcat atatatatta
     8761 tacaatttca atcaacaatt gttgtgcttt ttaaatgttg tttctatttc atttctaggg
     8821 tcgattttat tcgattctag ttttaaaatc gtgattttta acttctaacc ttactgagca
     8881 gactgaatga acatgtttca tcaagattaa cttcattata gttaattatt gaatcgtgct
     8941 agtggtttat aaatcagctc tatttaaatt aatcatcatc attatttcta tttaattttt
     9001 attatttctt taggattatt tttatgtgat ttttttcacg ttctagtttt aaactcaatg
     9061 ctcattactg ctcacccttg ctgagcagtc tttcgttaag attaaattaa ttaataacca
     9121 ttctagtttt aaaacagtgt cttaaggcat agctgatatt ttacaagttt actatttaat
     9181 atatctcctt tcttttttta gggtcgattt catctgattt gattaatttt tattatcttc
     9241 actaagcttt ttgttctagg ttttattctc agtattatta ctgttatatt tgaactgaat
     9301 ttctcctcat agtttattca ttttatttat ttattttttg tttattcgct attttgtatt
     9361 ttagttttta attagaaatt ttattagaat cgttttgaac tagtttttat gcactctaac
     9421 attaatatta aatcataact attattttta ttataattta tttgtgttta tctttttgtg
     9481 tagctttcat gtgtttttat tttagtttta ttatgtttgc attgaatttt ttggactagt
     9541 ttatataacg atatacagaa aagccatgac aacaaatgtt tttataatac ctattaagta
     9601 taagctaatc agatttccac tattctgttc aaagttaaca ttattagcca ttctgatgga
     9661 tcatttgaca tttgtcatat gtctttatga acactgctct ttcagatgcc acttttaaag
     9721 ctgtttttta cagtaaatga acgtttatta ttatgacatt atttcctaag gcctctagag
     9781 tacagcagga tgtggcgtgt gcattaccgt gtaattatgc tgactgtaaa tgtgcattta
     9841 tagactgtca gcatgtctga ctgtgcgctg ttgtgattgg tcctgcacag accaag
//
